Purpose:The aim of this study is to investigate the relationship between pain level and general comfort in palliative care patients. Methods: The study was conducted with 358 patients aged 18 years and older, conscious, open to verbal communication, with a Palliative Performance Score of 40% and above, who were hospitalized in the palliative care clinics of a public hospital between March 2019 and February 2020, and agreed to participate in the study. Data were collected with Personal Information Form, Palliative Performance Scale (PPS), Short Form -McGill Melzack Pain Questionnaire (SF-MPQ), and General Comfort Scale (GCS). Independent sample t-test, one-way analysis of variance (ANOVA), Pearson Correlation and Linear Regression analysis were used to analyze the data. Results: Of the participants, the mean age was 38.76±8.90 years, 53.4% were male, 29.6% were cancer patients, It was determined that 39.7% of them had a 4-6 day hospital stay history, and 70.7% of them had their daily life affected due to pain. It was determined that 21.2% of the patients had head extremity, 24.3% had throbbing, 36.6% had terrible pain, 45.3% had a rhythmic-periodic-intermittent pain. As a result of the research, it was determined that the pain intensity (cm) of the patients was 8.01±0.52, and the mean total score of the general comfort scale was 1.87±0.11. A high negative correlation was found between SF-MPQ and GCS (p<0.05). Conclusion: In this study, it was determined that an increase in the level of pain decreased the general comfort level in palliative care patients.