The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro

Dong, Dake; Jiang, Min; Xu, Xiaowei; Guan, Ming; Wu, Jiaqiang; Chen, Qinyi; Xiang, Leihong

doi:10.1016/j.jdermsci.2012.01.012

Cited by 31 publications

(22 citation statements)

References 28 publications

(28 reference statements)

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“…Another vitiligo treatment option, narrow band UVB (NB-UVB (311-nm)), is a relatively effective therapy and has been substituted for conventional PUVA therapy [87] . NB-UVB treatment induces Sox10 , Kit , and MC1R and enhances differentiation of melanocytes, possibly from the MelSCs [88] . The mechanism of repigmentation after UV has been also studied.…”

Section: Melanocyte Stem Cells In Human Skin Disordersmentioning

confidence: 99%

“…Although this method is not yet used in the clinical setting, the source of the melanocytes could be the MelSCs in ORS and this represents a promising method for biologic therapeutics in the near future. Moreover, with narrow band-UVB (NB-UVB) treatment, hair follicle NCSCs were directly affected and differentiated into a melanocyte lineage that produced pigmentation in vitro [88] . Such studies suggest that these MelSC reservoirs are important for repigmentation of the skin.…”

Section: Expert Opinion: Potential Clinical Applications Of Melanomentioning

confidence: 99%

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Melanocyte stem cells as potential therapeutics in skin disorders

Lee

Fisher

2014

Expert Opinion on Biological Therapy

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Introduction Melanocytes produce pigment granules that color both skin and hair. In the hair follicles melanocytes are derived from stem cells (MelSC) that are present in hair bulges or sub-bulge regions and function as melanocyte reservoirs. Quiescence, maintenance, activation, and proliferation of MelSC are controlled by specific activities in the microenvironment that can influence the differentiation and regeneration of melanocytes. Therefore, understanding MelSC and their niche may lead to use of MelSC in new treatments for various pigmentation disorders. Areas covered We describe here pathophysiological mechanisms by which melanocyte defects lead to skin pigmentation disorders such as vitiligo and hair graying. The development, migration, and proliferation of melanocytes and factors involved in the survival, maintenance, and regeneration of MelSC are reviewed with regard to the biological roles and potential therapeutic applications in skin pigmentation diseases. Expert Opinion MelSC biology and niche factors have been studied mainly in murine experimental models. Human MelSC markers or methods to isolate them are much less well understood. Identification, isolation and culturing of human MelSC would represent a major step toward new biological therapeutic options for patients with recalcitrant pigmentary disorders or hair graying. By modulating the niche factors for MelSC it may one day be possible to control skin pigmentary disorders and prevent or reverse hair graying.

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Section: Melanocyte Stem Cells In Human Skin Disordersmentioning

confidence: 99%

Section: Expert Opinion: Potential Clinical Applications Of Melanomentioning

confidence: 99%

Melanocyte stem cells as potential therapeutics in skin disorders

Lee

Fisher

2014

Expert Opinion on Biological Therapy

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“…NB-UVB was also shown to directly stimulate hair follicle-derived neural crest stem cells to differentiate into melanocyte lineage. NB-UVB enhanced the mobility of NCCmelan5 cells via upregulation of pp125FAK (a protein known to be involved in cell mobility after phosphorylation) as well as increased melanin formation and tyrosinase expression [61,63]. Remarkably, irradiation with higher NB-UVB dose (50 mJ/cm 2 ) decreased the viability of NCCmelan5 cells by about 30% [61].…”

Section: Narrow Band Uvb (311 Nm) Action On Other Cell Typesmentioning

confidence: 99%

Effects of Narrow Band UVB (311 nm) Irradiation on Epidermal Cells

Reich

Mędrek

2013

IJMS

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Ultraviolet radiation (UVR) is known to be one of the most important environmental hazards acting on the skin. It was revealed that chronic exposure to UVR accelerates skin aging, induces immunosuppression and may lead to the development of skin cancers. On the other hand, UVR has been shown to be effective in the treatment of numerous skin diseases and thus, various phototherapy modalities have been developed to date. Narrow-band ultraviolet B (NB-UVB) emitting a light with a peak around 311 nm has been demonstrated to be effective in the treatment of various skin disorders; currently it is one of the most commonly used phototherapy devices. Despite NB-UVB has been developed more than 30 years ago, the exact mechanism of its therapeutic action remains poorly understood. To date, most of NB-UVB effects were attributed to its influence on immune cells; however, nearly 90% of NB-UVB irradiation is absorbed by epidermis and keratinocytes seem to be important players in mediating NB-UVB biological activity. Here, we have reviewed the current data about the influence of NB-UVB on epidermal cells, with a special emphasis on cell proliferation and death.

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“…For UV irradiation, the narrow-band ultraviolet B (NB-UVB) radiation was performed as previously described [13]. For H2O2 treatment, HEKs were treated with were treated with LPS (concentration 0 μg/ml, 1 μg/ml, 5 μg/ml, 10 μg/ml, 50 μg/ml, and 100 μg/ml) for 24 h and 48 h, respectively.…”

Section: Cell Treatmentmentioning

confidence: 99%

“…Cell invasion was tested using nuclepore filters (8 mm pore size, Corning Inc., Corning, NY, USA) as described earlier [13].…”

Section: Cell Migration Assaymentioning

confidence: 99%