The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells

Evaluation of In-vitro Anti-Inflammatory Activity of Silver Nanoparticles Synthesised using Piper Nigrum Extract

“…Piperine inhibited the expression of IL6 and Matrix Metalloproteinase (MMP) 13 and reduced the production of PGE2 in a dose dependant manner at concentrations of 10 to 100 μg/ml [15]. In a study by Chuchawankul et al, Piperine also inhibited IL-2 and Interferon (IFN)-γ production by activated PBMCs in a dosedependent manner [16].…”

Silver Nanoparticles Interactions with the Immune System: Implications for Health and Disease

“…This opens up new avenues in the world of adjuvants and illustrates the basic requirements for the design of NP conjugates that efficiently reach their target. It has recently been studied whether AgNPs with a narrow size distribution and protected with a monolayer of adsorbed tiopronin (Ag@tiopronin) had any functional impact on specific TLR stimulation of Interleukin-6 (IL-6) secretion in Raw 264.7 macrophages, a murine monocyte/macrophage cell line (Castillo et al, 2008), and the effects of silver nanoparticles on cytokine expression by peripheral blood mononuclear cells (PBMC) (Shin et al, 2007), human mesenchymal stem cells (hMSCs) (Greulich et al, 2009), and J774 A1 macrophages (Yen et al, 2009 The results demonstrated that Ag@tiopronin do not show proinflammatory effects on macrophages and that interestingly, Ag@tiopronins differentially inhibits the IL-6 secretion mediated by specific TLRs located in the cell surface or in the endocytic compartments (Castillo et al, 2008).…”

“…In the case of J774 A1 macrophages the expression levels of IL-1 and IL-6 were similar to controls. The biological effects of nanoparticles were analyzed by enzyme-linked immunosorbent assay (ELISA) in the case of PBMCs stimulated by phytohaemagglutinin (PHA) in the presence of NPs (Shin et al, 2007) and hMSCs stimulated previously by lipopolysaccaride (LPS) (Greulich et al, 2009). In the case of non-stimulated J774 A1 macrophages, the cytokine mRNA expression levels were analyzed in cells treated with NPs and were compared to the resting state, measured by RT-PCR (Yen et al, 2009).…”

Biosynthesis of silver nanoparticles from mangrove plant (Avicennia marina) extract and their potential mosquito larvicidal property

“…Among the mangrove plants Rhizophora mucronata is previously proved to have antibacterial, antiplasmodial and antiviral activities (Premanathan et al 1999;Ravikumar et al 2009;Ravikumar et al 2011). Nanosized silver can also be used to treat immunologic and inflammatory diseases (Shin et al 2007). Currently, silver sulfadiazine is listed as an essential anti-infective topical medicine by the World Health Organization (WHO 2010).…”