The effects of moderate and low levels of acoustic overstimulation on stereocilia and their tip links in the guinea pig

Clark, Joseph A.; Pickles, James O.

doi:10.1016/s0378-5955(96)00092-5

Cited by 42 publications

(21 citation statements)

References 25 publications

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“…Moderate noise exposure causes tip link damage in birds and mammals (Clark and Pickles, 1996;Husbands et al, 1999). As tip links cannot be visualized using the fluorescent labeling method employed here, future studies may use scanning electron microscopy (SEM) to examine tip link structure under this noise exposure paradigm.…”

Section: Discussionmentioning

confidence: 99%

Anatomical and functional recovery of the goldfish (Carassius auratus) ear following noise exposure

Smith

Coffin

Miller

et al. 2006

Journal of Experimental Biology

129

128

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Many vertebrates, including fishes and marine mammals, rely on acoustic signals for communication. In aquatic environments, where sound propagates five times faster than in air, acoustic signals are particularly important for communication over long distances or in low-light environments. Research on a variety of fishes, however, demonstrates sensitive hearing capabilities even in nonvocalizing fishes, leading to the hypothesis that hearing in fishes evolved for analysis of the complete auditory scene, including both biotic (e.g.

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Section: Discussionmentioning

confidence: 99%

Anatomical and functional recovery of the goldfish (Carassius auratus) ear following noise exposure

Smith

Coffin

Miller

et al. 2006

Journal of Experimental Biology

129

128

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“…The hearing acuity of the guinea pigs was measured with clickevoked ABR before and at various intervals (1,4,7,14,21, and 63 days) after noise exposure. An evoked potential system (Spirit; Nicolet, Madison, Wisc., USA) was used to measure the threshold of the ABR.…”

Section: Auditory Brainstem Responsesmentioning

confidence: 99%

Changes of Hair Cell Stereocilia and Threshold Shift after Acoustic Trauma in Guinea Pigs: Comparison between Inner and Outer Hair Cells

Chen¹,

Liu²,

Cheng

et al. 2003

ORL

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The vulnerability of inner hair cells (IHCs) and outer hair cells (OHCs) to acoustic overstimulation is still controversially discussed. The present study was undertaken to investigate the vulnerability of IHCs and OHCs and the relation between chronological changes of auditory threshold shifts and stereocilia damages on IHCs and OHCs in guinea pigs after moderate acoustic trauma, caused by a single continuous exposure to pink noise (20 Hz to 20 kHz) of around 106 ± 2 dB SPL for 44 h. Stereocilia changes and threshold shifts of auditory brainstem responses (ABR) were assessed at regular intervals after noise exposure for 9 weeks. Scanning electron microscopy demonstrated the morphological changes of steriocilia as early as 1 day after noise exposure. The morphological changes included fused, bent, collapsed, and even missing stereocilia. These damages were more prominent on IHCs than on OHCs. The shift of ABR threshold was not parallel to the chronological change of the stereocilia on IHCs as well as OHCs. The elevation of the ABR threshold (40–60 dB SPL) was greatest on the 1st day after noise exposure, whereas the stereocilia showed the most damage 7 days after noise exposure. Combined with the results from previous studies, we conclude that moderate-level (around 105–110 dB) noise tends to induce more damage to the stereocilia of IHCs than of OHCs. Other damage (e.g., metabolic disturbance) than morphological damage of hair cell stereocilia may contribute partially to the hearing threshold shift induced by moderate acoustic overstimulation.

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“…Clark and Pickles (29) reported that many IHC stereocilia withstood a 30-min exposure of 115 dB SPL at 10 kHz; however, the stereocilia of the tallest row separated from the shorter stereocilia, although the shorter stereocilia remained interconnected by tip links. In the present analysis, under the condition in which the lateral links were included and a stimulus of 60 dB SPL at 500 Hz was applied to the model, the first tip link was always under a 1.19 times greater stress than the second tip link as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Dynamic Behavior of the Inner Hair Cell Stereocilia by the Finite Element Method

Matsui

Nakajima

Yamamoto

et al. 2006

JSME Int. J., Ser. C

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The motion of the inner hair cell (IHC) stereocilia, which results in tension in the tip links connected to mechanically gated ion channels, mediates the auditory transduction process. However, it is difficult to directly observe the motion of the stereocilia because of their minute dimensions and complex structure. In this study, to investigate such motion, a finite element method model of the tall, middle and short IHC stereocilia, including the tip and lateral links extending between the stereocilia, was constructed. By applying an analytically estimated fluid force caused by a stimulus of 60 dB SPL at 500 Hz to the model, the dynamic behavior of the stereocilia was analyzed. Numerical results showed that the stereocilia moved in phase and that the maximum tensions of 2.5 fN and 2.1 fN occurred in the tip link connecting the tall and middle stereocilia and in the tip link connecting the middle and short stereocilia, respectively. By contrast, under the condition in which the lateral links were removed, maximum tension in the former increased to 11.6 fN, while that in the latter only increased to 2.3 fN. It was therefore suggested that the lateral links protect the MET channels located at taller stereocilia against large stimuli and subject the channels located in the same IHC to forces of similar size.

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The effects of moderate and low levels of acoustic overstimulation on stereocilia and their tip links in the guinea pig

Cited by 42 publications

References 25 publications

Anatomical and functional recovery of the goldfish (Carassius auratus) ear following noise exposure

Anatomical and functional recovery of the goldfish (Carassius auratus) ear following noise exposure

Changes of Hair Cell Stereocilia and Threshold Shift after Acoustic Trauma in Guinea Pigs: Comparison between Inner and Outer Hair Cells

Analysis of the Dynamic Behavior of the Inner Hair Cell Stereocilia by the Finite Element Method

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