The effects of miR-1207-5p expression in peripheral blood on cisplatin-based chemosensitivity of primary gallbladder carcinoma

Abstract

ObjectiveThe aim of this study was to investigate the association between miR-1207-5p expression in peripheral blood and the chemosensitivity of primary gallbladder carcinoma (PGBC).MethodsA total of 85 patients with PGBC undergoing preoperative chemotherapy were divided into effective (n=18) and ineffective (n=67) groups. Another 70 healthy individuals were selected as the control group. An miR-1207-5p mimic (mimic group), an inhibitor (inhibitor group), and a negative control (NC group) sequence were transfected into human gallbladder carcinoma GBC-SD cells. Real-time quantitative polymerase chain reaction was used to determine miR-1207-5p expression. After 48 hours of cisplatin treatment, CCK-8 method was used to detect cell proliferation and flow cytometry were performed to examine cell apoptosis.ResultsmiR-1207-5p expression in peripheral blood was significantly associated with tumor node metastasis staging of PGBC (P<0.05). Before chemotherapy, miR-1207-5p expression in patients was higher than in healthy individuals (P<0.05). After chemotherapy, the effective group had lower miR-1207-5p expression than the ineffective group (P<0.05). The rates of positive expression of Ki67 protein in the effective group were significantly lower than those in the ineffective group (P<0.05). Receiver operating characteristic curves showed that the area under curve, sensitivity, and specificity of miR-1207-5p used to diagnose PGBC were 0.898, 77.6%, and 97.1% at a cutoff of 1.470, respectively. After 48 hours of cisplatin treatment, compared with the NC group and nontransfected (non-T) group, the mimic group had decreased rates of cell inhibition and apoptosis, but the inhibitor group had increased rates (all P<0.05). The expression levels of caspase3 protein were increased in the mimic group and decreased in the inhibitor group. Cell survival rates in the mimic group at different time points after cisplatin treatment were significantly higher than the corresponding rates in the NC and non-T groups, whereas the cell survival rates in the inhibitor group were significantly lower than the rates in the NC and non-T groups (all P<0.05). The concentration and action time of cisplatin were negatively associated with the cell survival rate in each group (all P<0.05).ConclusionCisplatin-based chemosensitivity of PGBC increased as expression of miR-1207-5p in peripheral blood declined. Thus, miR-1207-5p appears to be a promising and novel chemosensitizer for the treatment of PGBC.

Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis

“…MicroRNAs (miRNAs/miRs) are a class of non-coding small RNAs that bind to the 3'-untranslated region (3'-UTR) of mRNA to regulate mRNA expression (17)(18)(19). miRNAs have been proven to be involved in various cellular processes, including cell growth, differentiation and metabolic progression (20)(21)(22)(23)(24). The dysregulation of miRNAs has also been confirmed in AR, suggesting the potential function of miRNAs in AR (25,26).…”

miR‑1207‑5p regulates the sensitivity of triple‑negative breast cancer cells to Taxol treatment via the suppression of LZTS1 expression

“…These reports suggest a potential oncogenic role of miR-1207 in cancer. Moreover, a previous report about the effects of miR-1207-5p expression in peripheral blood on cisplatin-based chemosensitivity in primary gallbladder carcinoma patients, indicating the lower miR-1207-5p expression in effective group than in the ineffective group after chemotherapy (26), suggesting its role in chemosensitivity. Therefore, the present study first detected the expression level of miR-1207-5p in normal MCF-10A cells and TNBC MDA-MB-436, MDA-MB-453 and MDA-MB-231 cell lines, and found that, compared with normal MCF-10A cells, there was a higher miR-1207-5p expression level in the TNBC cell lines, with the highest level being observed in the MDA-MB-231 cells.…”

Update on Chemoresistance Mechanisms to First-Line Chemotherapy for Gallbladder Cancer and Potential Reversal Strategies

“…In addition, lnc-RNA-MYLK-AS1 promotes GEM resistance by upregulating EZH expression 19. Other studies report that MiR-218-5p promotes GBC sensitivity to GEM by attenuating PRKCE expression 24,75. However, MiR-125b-5p enhances GBC sensitivity to cisplatin by downregulating Bcl-2 25,41.…”

“… 19 Other studies report that MiR-218-5p promotes GBC sensitivity to GEM by attenuating PRKCE expression. 24 , 75 However, MiR-125b-5p enhances GBC sensitivity to cisplatin by downregulating Bcl-2. 25 , 41 GBC sensitivity to cisplatin was shown to increase when MiR-1207-5p expression decreased in the peripheral blood.…”