The Effects of Low-Level Laser Therapy on Palatal Mucoperiosteal Wound Healing and Oxidative Stress Status in Experimental Diabetic Rats

Firat, Ela Tules; Dağ, Ahmet; Günay, Ahmet; Kaya, Beyza; Karadede, Mehmet İrfan; Kanay, Berna Ersöz; Ketani, Aydın; Evliyaoğlu, Osman; Uysal, Ersin

doi:10.1089/pho.2012.3406

Cited by 29 publications

(22 citation statements)

References 44 publications

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“…Therefore, the initial inflammatory response after injury is important for rapid wound healing. In a pathological condition, such as that of diabetics, slow removal of necrotic tissue delays the onset of healing and results in chronic inflammation [8,9]. Angiogenesis, the formation of new vessels from preexisting ones, is essential to successful wound healing.…”

Section: Introductionmentioning

confidence: 99%

Effect ofHominis Placentaon cutaneous wound healing in normal and diabetic mice

Park

Lee

Jeong³

et al. 2014

Nutr Res Pract

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BACKGROUND/OBJECTIVESThe number of diabetic patients has recently shown a rapid increase, and delayed wound healing is a major clinical complication in diabetes. In this study, the wound healing effect of Hominis placenta (HP) treatment was investigated in normal and streptozotocin-induced diabetic mice.MATERIALS/METHODSFour full thickness wounds were created using a 4 mm biopsy punch on the dorsum. HP was injected subcutaneously at the middle region of the upper and lower wounds. Wounds were digitally photographed and wound size was measured every other day until the 14th day. Wound closure rate was analyzed using CANVAS 7SE software. Wound tissues were collected on days 2, 6, and 14 after wounding for H/E, immunohistochemistry for FGF2, and Masson's trichrome staining for collagen study.RESULTSSignificantly faster wound closure rates were observed in the HP treated group than in normal and diabetes control mice on days 6 and 8. Treatment with HP resulted in reduced localization of inflammatory cells in wounded skin at day 6 in normal mice and at day 14 in diabetic mice (P < 0.01). Expression of fibroblast growth factor (FGF) 2 showed a significant increase in the HP treated group on day 14 in both normal (P < 0.01) and diabetic mice (P < 0.05). In addition, HP treated groups showed a thicker collagen layer than no treatment groups, which was remarkable on the last day, day 14, in both normal and diabetic mice.CONCLUSIONSTaken together, HP treatment has a beneficial effect on acceleration of cutaneous wound healing via regulation of the entire wound healing process, including inflammation, proliferation, and remodeling.

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Section: Introductionmentioning

confidence: 99%

Effect ofHominis Placentaon cutaneous wound healing in normal and diabetic mice

Park

Lee

Jeong³

et al. 2014

Nutr Res Pract

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“…It is a well-known fact that the sensitivity of cells depends on the rate of proliferation and degree of differentiation of cells. 25 Laser irradiating at a wavelength of 940 nm increases proliferation and differentiation of cells, [21][22][23]26 the same as other…”

Section: Discussionmentioning

confidence: 87%

“…19,20 Total oxidative status is likewise decreased after low-level laser treatment. 21 This apparenty provides a suitable basis for radioprotective feature of laser light. Accordingly, irradiation of therapeutic laser is possible regulative method of cellular radiosensitization which make cells more protective against changes in the extracellular environment.…”

mentioning

confidence: 95%

“…23 The number of inflammatory cells on day 1 and the mitotic activity of fibroblasts on day 7 and 14 are significantly increased after the end of laser therapy. 21,23 On the other hand, several studies indicate that a 940 nm laser has no impact on collagen synthesis or vascularization 21,23 However, after 940 nm laser treatment, insulin-like growth factor, vascularendothelial growth factor, and transforming growth factorbeta mRNA are increased. 24 The possible radioprotective effect of laser may be widely used in oncology and radiology when an irradiated organism must be protected from ionizing radiation.…”

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confidence: 99%

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Protective Effect of 940 nm Laser on Gamma-Irradiated Mice

Efremova

Šinkorová

Navrátil

2015

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to investigate the radioprotective features of 940 nm laser on the life span of mice, and absolute counts of blood cells and their proportions in gamma-irradiated mice. Background data: An important feature of laser light is activation of mitotic division and differentiation of cells, which may be useful in activation of hematopoiesis in gamma-irradiated organisms. Materials and methods: Mice were randomly assigned to 11 groups according to the type(s) of influence. Generally, mice were irradiated in three different ways: with laser at different fluences, with gamma irradiation, or by combination of laser at different fluences and gamma irradiation in a different order. Mice were treated with 940 nm laser at 3, 12, or 18 J/cm 2 and/or a lethal dose of gamma irradiation (8.7 Gy). Each group was randomly subdivided into two subgroups, in which the life span of the mice and blood cell counts (on 12th and 45th day after gamma irradiation) were analyzed. Results: Laser (940 nm) at a fluence of 3 J/cm 2 significantly prolonged the life span of gammairradiated mice ( p < 0.05). In the same group, counts of white blood cells, lymphocytes, and neutrophils were higher on day 12 than in the gamma group. On day 45 after gamma irradiation, some signs of hematopoiesis repair were found in blood. There were no significant differences in counts of erythrocytes, monocytes, neutrophils, or the proportion of neutrophils between this group and the control group. Conclusions: In summary, 940 nm laser at a fluence of 3 J/cm 2 demonstrates radioprotective features in an experiment with lethally irradiated mice. Mechanisms responsible for this effect will be investigated in further studies.

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“…It may be postulated that low-level laser modulation of the reduced glutathione levels could affect the generation of reactive oxygen species. There are a few reports confirming the antioxidant effect of LLLT based on the changes in the redox status [9, 10]. …”

Section: Discussionmentioning

confidence: 99%

Effect of the transdermal low-level laser therapy on endothelial function

et al. 2016

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The effect of low-level laser therapy (LLLT) on the cardiovascular system is not fully established. Since the endothelium is an important endocrine element, establishing the mechanisms of LLLT action is an important issue.The aim of the study was to evaluate the effect of transdermal LLLT on endothelial function.In this study, healthy volunteers (n = 40, age = 20–40 years) were enrolled. N = 30 (14 female, 16 male, mean age 30 ± 5 years) constituted the laser-irradiated group (LG). The remaining 10 subjects (6 women, 4 men, mean age 28 ± 5 years) constituted the control group (CG). Participants were subjected to LLLT once a day for three consecutive days. Blood for biochemical assessments was drawn before the first irradiation and 24 h after the last session. In the LG, transdermal illumination of radial artery was conducted (a semiconductor laser λ = 808 nm, irradiation 50 mW, energy density 1.6 W/cm2 and a dose 20 J/day, a total dose of 60 J). Biochemical parameters (reflecting angiogenesis: vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), angiostatin; antioxidative status: glutathione (GSH) and the nitric oxide metabolic pathway: symmetric dimethylarginine (SDMA), asymmetric dimethylarginine (ADMA) and l-arginine) were assessed. In the LG, a significant increase in GSH levels and considerable decrease in angiostatin concentration following the LLLT were observed. No significant differences in levels of the VEGF, FGF, SDMA, ADMA were observed.LLLT modifies vascular endothelial function by increasing its antioxidant and angiogenic potential. We found no significant differences in levels of the nitric oxide pathway metabolites within 24 h following the LLLT irradiation.

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The Effects of Low-Level Laser Therapy on Palatal Mucoperiosteal Wound Healing and Oxidative Stress Status in Experimental Diabetic Rats

Abstract: LLLT elicits a positive healing effect on palatal mucoperiostal wounds, and modulates the oxidative status in experimental diabetic rats.

Cited by 29 publications

References 44 publications

Effect ofHominis Placentaon cutaneous wound healing in normal and diabetic mice

Effect ofHominis Placentaon cutaneous wound healing in normal and diabetic mice

Protective Effect of 940 nm Laser on Gamma-Irradiated Mice

Effect of the transdermal low-level laser therapy on endothelial function

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