The effects of low-dose radiation in the treatment of sciatic nerve injury in rats

Göçmen, Selçuk; Şirin, Sait; Oysul, Kaan; Ulaş, Ümit Hıdır; Öztaş, Emin

doi:10.5137/1019-5149.jtn.5039-11.1

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…1). 17,18 The site was rinsed with sterile saline solution, the muscle and skin layers were sutured with absorbable Vicryl 4-0 (Ethicon), and the wound was disinfected with Betadine (Mundipharma Medical, Hamilton, Bermuda). After 2 weeks, therapy was initiated as described earlier.…”

Section: Methodsmentioning

confidence: 99%

Regeneration of nerve crush injury using adipose‐derived stem cells: A multimodal comparison

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Regeneration of nerve crush injury using adipose‐derived stem cells: A multimodal comparison

et al. 2018

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“…In the treatment of peripheral nerve injuries, suturing with vinyl and silk was done first; however in light of the number of sutures and the negative effects of the sutures on recovery, fibrin glue and wrapping the injured nerve were also tried. Electrical stimulation, therapeutic ultrasound, low-dose radiation, and low-intensity laser were also tested in experimental models to produce nerve regeneration and functional recovery (8,13,15,16,17).…”

Section: I-light Microscopic Evaluationmentioning

confidence: 99%

Neuroprotective effects of agmatine in experimental peripheral nerve injury in rats: a prospective randomized and placebo-controlled trial

Sezer

Güçlü²,

Kazanci³

et al. 2013

Turkish Neurosurgery

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AIm:The purpose of this study was to demonstrate the activity of agmatine, an inducible nitric oxide synthase (iNOS) inhibitor and selective N-methyl-D-aspartate receptor (NMDAR) antagonist, on reducing tissue damage in distal part of traumatic nerve in an experimental rat peripheral nerve injury model. mATeRIAL and meTHods: Sciatic nerves of 30 Sprague Dawley male rats were used. Rats were divided into 5 groups; group 1 (n=6), control group; group 2 (n=6), axonotmesis + placebo group; group 3 (n=6), axonotmesis + 50 mg/kg agmatine treatment group; group 4 (n=6), neurotmesis + placebo group; group 5 (n=6), neurotmesis + 50 mg/kg agmatine treatment group. Axonolysis, axon degeneration, edema, hemorrhage, and inflammation were evaluated in histopathologic examinations of all the groups.ResuLTs: When group 2 was compared with group 3 in histopathologic sections, axonolysis was less in group 3 (p=0.007), as was axon degeneration (p=0.022) and edema (p=0.018). When group 4 was compared with group 5, axonolysis was less in group 5 (p=0.009), as was axon degeneration (p=0.006) and edema (p=0.021). CoNCLusIoN:This study demonstrated agmatine to have antioxidant and antineurotoxic effects in an experimental rat peripheral nerve injury model. KeywoRds: Agmatine, Axonotmesis, Neurotmesis, Peripheral nerve injury, Rat ÖZ AmAÇ: Bu çalışmanın amacı, indüklenebilir nitrik oksit sentetaz inhibitörü (iNOS) ve selektif N-metil-D-aspartat reseptörü (NMDAR) antagonisti olan agmatinin sıçanlarda yapılan deneysel periferik sinir yaralanma modelinde hasarlı sinirin distal bölumündeki doku hasarını azaltmadaki etkisini göstermekti. yÖNTem ve GeReÇLeR: Otuz tane Sprague Dawley sıçanının siyatik sinirleri kullanıldı. Sıçanlar beş gruba bölündü: birinci grup (n=6), kontrol grubu; ikinci grup (n=6), aksonotmezis + plasebo grubu; üçüncü grup (n=6), aksonotmezis + 50 mg/kg agmatin tedavi grubu; dördüncü Grup (n=6), neurotmesis + plasebo grubu; beşinci grup (n=6), neurotmesis + 50 mg/kg agmatin tedavi grubu. Aksonolizis, akson dejenerasyonu, ödem, hemoraji ve inflammasyon bütün gruplarda histopatolojik olarak değerlendirildi.BuLGuLAR: İkinci grup ile üçüncü grup histopatolojik kesitlerde kıyasladığında, aksonolizis üçüncü grupta daha azdı (p=0,007), aynı şekilde akson dejenerasyonu (p=0,022) ve ödem (p=0,018) üçüncü grupta daha azdı. Dördüncü grup ile beşinci grup histopatolojik kesitlerde kıyasladığında, aksonolizis beşinci grupta daha azdı (p=0,009), aynı şekilde akson dejenerasyonu (p=0,006) ve ödem (p=0,021) beşinci grupta daha azdı. soNuÇ: Bu çalışma, agmatinin sıçanlarda yapılan deneysel periferik sinir yaralanmalarında antioksidan ve antinörotoksik etkileri olduğunu göstermiştir.

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“…Traffic accidents, firearm injuries, chemical injuries, cutting tool injuries, and crush traumas are some of the traumas that can be counted as the main causes ( 1 ). Although the most effective treatment after peripheral nerve trauma seems to be surgical repair (nerve anastomoses, flap, free-fat graft, and silicone cuffing), several healing-stimulating agents have been tested both in vitro and in clinical trials, although functional nerve regeneration with them has often been found insufficient ( 10 – 12 ). After inducing a sciatic nerve injury, functional response to medication can be evaluated by performing extremity changes and walking analysis in rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of tamoxifen therapy on sciatic nerve crush injury: An experimental study in rats

Akar

Emon

Efendioğlu

et al. 2021

AOTT

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Objective This study aimed to determine the effects of tamoxifen on sciatic nerve crush injury in a rat model using histopathological, histomorphometric, and immunohistochemical approaches. Methods In this study, 24 male Sprague-Dawley rats aged of 5 to 7 weeks and weighing between 300 g and 400 g were used. The rats were randomly divided into 3 groups: control (group C), sciatic nerve injury (group SNI), and sciatic nerve injury with tamoxifen (group SNT). The sciatic nerve crush injury model was performed using the De Koning’s crush force method. In group C, only a skin incision was made and then the skin was sutured. In group SNI, the injury model was performed but no treatment was applied. In group SNT, the injury model was executed, and then 40 mg/kg/day tamoxifen was given for 4 weeks by intraperitoneal methods. At the end of 4 weeks, all animals were killed using high doses of an anesthetic. Approximately, 2-cm sciatic nerve samples were obtained for histopathological, histomorphometric, and immunohistochemical analyses using the old skin incision. Results In the histopathological examination, vascular congestion and density of vacuolization were significantly lower in group SNT than in group SNI (p<0.05). In the histomorphometric examination, the mean sciatic nerve diameter was 306±62 μm in group C, 510±42 μm in group SNI, and 204±23 μm in group SNT. A significant difference was observed in the sciatic nerve diameter measurements among the 3 groups (p<0.05). In pairwise comparisons, the mean sciatic nerve diameter was significantly lower in group SNT than in group SNI (p=0.00002). Sciatic nerve diameter measurements of both groups were found to be significantly higher than group C (p<0.05). The mean epineurium thickness was 17±0.8 μm in group C, 32±2.5 μm in group SNI, and 17±0.8 μm in group SNT. A significant difference was observed in the epineurium thickness measurements among the 3 groups (p<0.05). In pairwise comparisons, the epineurium thickness was significantly higher in group SNI than in groups SNT and C (p<0.05). In the immunohistochemical analysis, S100 immunoreactivity was found significantly higher in group SNI than in the other 2 groups (p<0.05). Conclusion The histomorphometric, histopathological, and immunohistochemical data obtained from this study have shown that tamoxifen has a beneficial effect on sciatic nerve crush injury in the experimental rat model.

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The effects of low-dose radiation in the treatment of sciatic nerve injury in rats

Cited by 6 publications

References 27 publications

Regeneration of nerve crush injury using adipose‐derived stem cells: A multimodal comparison

Regeneration of nerve crush injury using adipose‐derived stem cells: A multimodal comparison

Neuroprotective effects of agmatine in experimental peripheral nerve injury in rats: a prospective randomized and placebo-controlled trial

Effects of tamoxifen therapy on sciatic nerve crush injury: An experimental study in rats

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