The effects of lidocaine and procaine on microRNA expression of adipocyte-derived adult stem cells

Abstract

BackgroundThe microRNA (miRNA) pathway has emerged as one of the biologic pathways implicated in stem cell regulation. miRNA is a noncoding, single-stranded RNA consisting of 20-25 nucleotides that inhibits the protein production at the step of translation. The molecular effects of lidocaine and procaine on adipose stem cells were investigated by examining RNA expression array.MethodsAdipose stem cells were isolated from a prior abdominal liposuction procedure. The human adipose stem cells were cultured and then added to a mixture of 1 ml of culture medium plus 1 ml of 2% lidocaine or 2% procaine for the duration of 30 minutes. The expression levels of miRNAs were estimated by using peptide nucleic acid (PNA)-miRNA array analysis throughout the denaturation and hybridization processes after the isolation of miRNA. The miRNAs detected by microarray that either decreased by half fold or increased by 1.5 fold from the control level were interpreted as significant.ResultsAccording to microarray analysis there were 61 miRNAs in total, and no miRNA had decreased expression levels. The stem cells treatment with lidocaine showed 4 alteration of expression with miR-9a* (1.53 fold), miR-29a (1.64 fold), miR-296-5p (1.64 fold) and miR-373 (1.94 fold). The stem cells treated with procaine showed 32 miRNAs that were significantly up-regulated with a range of 1.5 to 2.06 fold. They were stem cell differentiation-related miRNAs, apoptosis and cell cycle-associated miRNAs, immunity-associated miRNAs and hormonal response-related miRNAs.ConclusionsLidocaine and procaine affect the miRNA expression on adipose stem cells and the effect of procaine is more marked than that of lidocaine.

Effect of Lidocaine on Viability and Gene Expression of Human Adipose–derived Mesenchymal Stem Cells: An in vitro Study

“…Sung and coworkers investigated the relationship between MSCs and expression levels of microRNAs (miRNAs) using peptide nucleic acid (PNA)‐miRNA array analysis. This study demonstrated that lidocaine treatment of MSCs affects miRNA expression and that the effect of procaine is more significant than the one of lidocaine . The effects of lidocaine on human MSC functions have been addressed previously, but there were no studies examining their recovery following exposure .…”

“…The cytotoxicity of amide‐type local anesthetics including bupivacaine, ropivacaine, mepivacaine, and lidocaine to MSCs in both human and animal models has been reported in previous studies . However, only a few investigators have reported deleterious cytotoxic effects of varying concentrations of local anesthetics on MSCs after different exposure and assessment times . Sung and coworkers investigated the relationship between MSCs and expression levels of microRNAs (miRNAs) using peptide nucleic acid (PNA)‐miRNA array analysis.…”

“…43,44,46,47,53 However, only a few investigators have reported deleterious cytotoxic effects of varying concentrations of local anesthetics on MSCs after different exposure and assessment times. 47,54 Sung and coworkers investigated the relationship between MSCs and expression levels of microRNAs (miRNAs) using peptide nucleic acid (PNA)-miRNA array analysis. This study demonstrated that lidocaine treatment of MSCs affects miRNA expression and that the effect of procaine is more significant than the one of lidocaine.…”

“…This study demonstrated that lidocaine treatment of MSCs affects miRNA expression and that the effect of procaine is more significant than the one of lidocaine. 54 The effects of lidocaine on human MSC functions have been addressed previously, but there were no studies examining their recovery following exposure. 30 In addition, published data on changes in mRNA expression in MSCs treated with lidocaine are limited.…”

Anestesia Local Odontológica y su Influencia en Anomalías del Desarrollo Dental: Revisión Sistemática de la Literatura

“…Por otra parte Harato et al (2012) establecen que bupivacaína induce apoptosis en la línea celular Neuro2a por medio de síntesis de sustancias reactivas del oxígeno (ROS) y activación de p38 MAPK. A su vez ha sido observada la citotoxicidad de lidocaína (Jacobs et al, 2011) y procaína (Park et al, 2011) en células cartilaginosas; y de bupivacaína en células madres adiposas (Sung et al, 2012).…”