The effects of Lactobacillus plantarum with feruloyl esterase-producing ability or high antioxidant activity on the fermentation, chemical composition, and antioxidant status of alfalfa silage

Li, Fuhou; Ding, Zitong; Chen, X.Z.; Zhang, Y.X.; Ke, Wencan; Zhang, X.; Li, Z.Q.; Usman, Samaila; Guo, Xusheng

doi:10.1016/j.anifeedsci.2021.114835

Cited by 19 publications

(25 citation statements)

References 43 publications

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“…To the best of our knowledge, no native (hemi)cellulolytic LAB from Actinobacteria phylum has been previously reported. Interestingly, we also found that Bin002_S contained two carbohydrate esterase (CE1) enzymes that may improve the digestibility of forage within the rumen ( Li et al, 2021 ). However, the presence of FAE was not detected within the reconstructed MAGs.…”

Section: Discussionmentioning

confidence: 90%

Top-Down Enrichment Strategy to Co-cultivate Lactic Acid and Lignocellulolytic Bacteria From the Megathyrsus maximus Phyllosphere

et al. 2021

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Traditionally, starting inoculants have been applied to improve ensiling of forage used for livestock feed. Here, we aimed to build up a bioinoculant composed of lactic acid-producing and lignocellulolytic bacteria (LB) derived from the Megathyrsus maximus (guinea grass) phyllosphere. For this, the dilution-to-stimulation approach was used, including a sequential modification of the starting culture medium [Man, Rogosa, and Sharpe (MRS) broth] by addition of plant biomass (PB) and elimination of labile carbon sources. Along 10 growth-dilution steps (T1–T10), slight differences were observed in terms of bacterial diversity and composition. After the sixth subculture, the consortium started to degrade PB, decreasing its growth rate. The co-existence of Enterobacteriales (fast growers and highly abundance), Actinomycetales, Bacillales, and Lactobacillales species was observed at the end of the selection process. However, a significant structural change was noticed when the mixed consortium was cultivated in higher volume (500ml) for 8days, mainly increasing the proportion of Paenibacillaceae populations. Interestingly, Actinomycetales, Bacillales, and Lactobacillales respond positively to a pH decrease (4–5), suggesting a relevant role within a further silage process. Moreover, gene-centric metagenomic analysis showed an increase of (hemi)cellulose-degrading enzymes (HDEs) during the enrichment strategy. Reconstruction of metagenome-assembled genomes (MAGs) revealed that Paenibacillus, Cellulosimicrobium, and Sphingomonas appear as key (hemi)cellulolytic members (harboring endo-glucanases/xylanases, arabinofuranosidases, and esterases), whereas Enterococcus and Cellulosimicrobium have the potential to degrade oligosaccharides, metabolize xylose and might produce lactic acid through the phosphoketolase (PK) pathway. Based on this evidence, we conclude that our innovative top-down strategy enriched a unique bacterial consortium that could be useful in biotechnological applications, including the development/design of a synthetic bioinoculant to improve silage processes.

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Section: Discussionmentioning

confidence: 90%

Top-Down Enrichment Strategy to Co-cultivate Lactic Acid and Lignocellulolytic Bacteria From the Megathyrsus maximus Phyllosphere

et al. 2021

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“…In this study, linear effect of soybean inclusion × inoculation significantly improved both glucose yield and cellulose conversion of the silages (p < 0.05; Figure 5a,b). The higher glucose yield and cellulose conversion in these treatments were obviously due to enhanced fermentation and fiber degradation activity of L. plantarum A1 during ensilage [12,14,22,32]. The higher degradability of soybean fiber is the reason for the linear increase in GY and CC with increasing soybean inclusion during enzymatic saccharification.…”

Section: Enzymatic Saccharification Of Soybean Incorporated Sorghum S...mentioning

confidence: 90%

“…Biological inoculants have recently gained much attention. Several studies have reported the efficacy of lactic acid bacteria (LAB) for improved silage fermentation [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…The efficiency of L. plantarum A1 in improving the fermentation quality and lignocellulose degradation, as well as ferulic acid production of different biomass have previously been reported [12,14,22,32]. However, despite the higher cellulosic content of sorghum, and its ensiling potential as well as its potential for biofuel production, there is no report from any study on inoculating whole crop sorghum biomass with any feruloyl-esterase producing lactobacillus during ensiling.…”

Section: Introductionmentioning

confidence: 99%

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Lignocellulose Degradation and Enzymatic Hydrolysis of Soybean Incorporated Sorghum Silage Inoculated with Feruloyl-Esterase Producing Lactobacillus plantarum

Usman

Shou

et al. 2022

Fermentation

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Availability of feedstock is one of the uncertainties impeding cellulosic biofuel production, and conservation of whole crop biomass as silage is a promising method to ensure year-round feedstock availability for biofuel production. This study investigated lignocellulose degradation and subsequent enzymatic hydrolysis of a 90-d sorghum silage incorporated with soybean and inoculated with Lactobacillus plantarum A1 in a three (0, 25, and 50%; soybean inclusions) by two (uninoculated and inoculated) factorial experiment. The results revealed that L. plantarum A1 inoculated silages had improved fermentation characteristics. The silages’ total N and non-fiber carbohydrate increased with L. plantarum A1 and soybean inclusion (p < 0.05). Inoculation also increased the residual water-soluble carbohydrate by 33.9% (p < 0.05). Inoculation and soybean inclusion significantly hydrolyzed the biomass’ lignocellulose, altered its morphology and microstructural matrix, increased production of ferulic acid and reduced the biomass crystallinity by 15.60% (p < 0.05). L. plantarum A1 inoculation × soybean inclusion improved glucose yield and cellulose conversion during enzymatic saccharification compared to uninoculated treatments. Therefore, incorporating soybean into sorghum silage with L. plantarum A1 inoculation enhanced fermentation quality, lignocellulose degradation and enzymatic saccharification which could serve as a sure way for sustainable year-round feedstock supply for enhanced biofuel production.

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“…Total mixed ration (TMR) because of the high water content and the large proportion of silage raw materials, aerobic microorganisms are more active, and the quality stability is usually poor, so it is not suitable for storage ( Li et al, 2021 ). In the process of making and taking silage, there is often the problem of secondary fermentation, which will cause problems and losses in its economic benefits and popularization and application.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative properties analysis of gastrointestinal lactic acid bacteria in Hainan black goat and its effect on the aerobic stability of total mixed ration

Yang

Tang

et al. 2022

Front. Microbiol.

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In this study, lactic acid bacteria strains (HCS-01, HCS-05, HCS-07, HCW-08, and HCW-09) derived from the gastrointestinal tract of Hainan black goat were evaluated for their antioxidant capacity in vitro, and the lactic acid bacteria with strong antioxidant capacity were screened for application to improve the aerobic stability of total mixed ration (TMR). The results showed that all the tested lactic acid bacteria had a certain tolerance to hydrogen peroxide. By comprehensively comparing the scavenging abilities of fermentation supernatants, whole cell bacterial suspensions and cell contents of five lactic acid bacteria strains to 2,2-diphenyl-1-picrylhydrazine (DPPH), hydroxyl radicals and superoxide anions, and their antioxidant enzyme activity, it was found that Lactobacillus fermentum HCS-05 and Lactobacillus plantarum HCW-08 have the strongest comprehensive antioxidant capacity, and their scavenging capacity for various free radicals has reached more than 60%. Using strains HCS-05, HCW-08 and laboratory-preserved Lactobacillus plantarum HDX1 fermented TMR, the fermentation quality and aerobic stability of the feed after 60 days of fermentation were significantly higher than those of the blank treatment group. The effect of mixed strains HCS-05 and HCS-08 for TMR fermentation was the best (P < 0.05). At the same time, the fermentation effect of Lactobacillus plantarum HDX1 on TMR was significantly lower than that of the selected lactic acid bacteria from the gastrointestinal tract of Hainan black goats (P < 0.05). The results show that the test strain can significantly improve the aerobic stability of the fermented feeds.

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The effects of Lactobacillus plantarum with feruloyl esterase-producing ability or high antioxidant activity on the fermentation, chemical composition, and antioxidant status of alfalfa silage

Cited by 19 publications

References 43 publications

Top-Down Enrichment Strategy to Co-cultivate Lactic Acid and Lignocellulolytic Bacteria From the Megathyrsus maximus Phyllosphere

Top-Down Enrichment Strategy to Co-cultivate Lactic Acid and Lignocellulolytic Bacteria From the Megathyrsus maximus Phyllosphere

Lignocellulose Degradation and Enzymatic Hydrolysis of Soybean Incorporated Sorghum Silage Inoculated with Feruloyl-Esterase Producing Lactobacillus plantarum

Antioxidative properties analysis of gastrointestinal lactic acid bacteria in Hainan black goat and its effect on the aerobic stability of total mixed ration

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