The Effects of Korean Pine and Manchurian Walnut Monocultures and Mixed Plantations on Soil Fungal and Bacterial Communities

Abstract

(1) Background: Korean pine (Pinus koraiensis) and Manchurian walnut (Juglans mandshurica) are the main tree species for plantation regeneration in Northeast China, and the mixed plantation of them is one of the typical measures adopted to address the decline in stand productivity in long-term monocultures. However, little is known about the effects of Korean pine and Manchurian walnut monocultures and mixed plantations on soil microbial diversity, composition, and functional groups. (2) Methods: We used ITS and 16S rRNA gene sequencing to detect fungal and bacterial communities and used the FUNGuild, FAPROTAX, and Bugbase databases to predict their functional groups. (3) Results: Fungal and bacterial alpha diversity were always higher in Manchurian walnut monocultures than in Korean pine monocultures. The plantation type had a greater impact on the fungal composition than the bacterial composition. The fungal functional groups were significantly affected by the plantation type (p < 0.05), while the bacterial functional groups were barely changed among all plantation types. The soil available nutrient content was the most important soil factor in shaping the microbial community structures and functional groups. (4) Conclusions: Shifts in fungal community compositions and functional groups might play a dominant role in soil nutrient cycling across the different plantation types in Northeast China.