The effects of ionophore A23187 on erythrocytes relationship of ATP and 2,3-diphosphoglycerate to calcium-binding capacity

Edmondson, James W.; Li, Ting‐Kai

doi:10.1016/0005-2736(76)90494-6

Cited by 18 publications

(3 citation statements)

References 19 publications

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“…Potassium permeability has been shown to be related to intracellular calcium levels in a number of ceils (15,16), and we have previously presented evidence supporting calcium-sensitive potassium permeability changes in macrophages (9). It is of interest that A23187, which produces potential changes similar to those produced by C5a in macrophages, also produces a large potassium efflux in erythrocytes (8) and probably in leukocytes (9).…”

Section: Discussionmentioning

confidence: 79%

Interaction of chemotactic factors with human macrophages: induction of transmembrane potential changes

Gallin¹,

Gallin²

1977

The Journal of Cell Biology

166

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The electrophysiology of chemotactic factor interaction with cultured human macrophages was investigated with standard intracellular recording techniques. In initial studies, E. coli endotoxin-activated serum, added to cell cultures during intracellular recordings, caused membrane hyperpolarizations which were greater than 30 s in duration, 10-50 mV in amplitude, and associated with decreased membrane resistance. Control serum produced smaller hyperpolarizations lasting 10-20 s and 5-30 mV in amplitude. Endotoxin-activated human serum deficient in the third complement component (C3) did not produce hyperpolarizations unless the serum was reconstituted with C3 before activation. Fractionation of normal activated serum by molecular seive chromatography (G-75 Sephadex) indicated that only fractions that eluted with an estimated molecular weight of 12,500 produced membrane potential changes. The active material that was chemotactic for the macrophages was identified as the small molecular weight cleavage product of C5, C5a, by heat stability (30 min at 56~ and inactivation by goat antisera to human C5 but not C3. 17% of macrophages stimulated with C5a exhibited a biphasic response characterized by a small (2-6 mV), brief (1-10 s) depolarization associated with a decreased membrane resistance preceding the larger and prolonged hyperpolarizations. Magnesium-ethylene glycol bis [fl-aminoethyl ether]N, N'-tetraacetic acid (Mg [2.5 mM]-EGTA [5.0 raM]) blocked the CSaevoked potential changes, whereas colchine (10 -6 M) and cytochalasin B (3.0 p,g/ ml) did not. Hydrocortisone sodium succinate (0.5 mg/ml) decreased the percentage of cells responding to C5a. In related studies, synthetic N-formyl methionyl peptide (f-met-leu-phe), which had chemotactic activity for cultured macrophages, produced similar membrane potential changes. Repeated exposure of macrophages to C5a or f-met-leu-phe resulted in desensitization to the same stimulus. Simultaneous photomicroscope and intracellular recording studies during macrophage stimulation with chemotactic factor demonstrated that the membrane potential changes preceded membrane spreading, ruffling, and pseudopod formation. These observations demonstrate that ion fluxes associated with membrane potential changes are early events in macrophage activation by chemotactic factors.

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Section: Discussionmentioning

confidence: 79%

Interaction of chemotactic factors with human macrophages: induction of transmembrane potential changes

Gallin¹,

Gallin²

1977

The Journal of Cell Biology

166

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“…Red blood cells (RBCs) of healthy human adults contain ϳ0.2 M Ca 2ϩ , which is ϳ10 4 -fold smaller than the plasma Ca 2ϩ concentration of 1.8 mM. Minor shifts in intracellular calcium ion concentration, [Ca 2ϩ ] i , produce significant changes in the biological properties of RBCs (Edmonson and Li, 1976;Allan and Thomas, 1981a,b;Allan et al, 1989;Friederichs et al, 1989;Hagelberg and Allan, 1990), and excessive elevations of [Ca 2ϩ ] i are deleterious to cell function and survival. The [Ca 2ϩ ] i of intact RBCs is controlled by the inward diffusion of Ca 2ϩ across the plasma membrane and the outward extrusion of Ca 2ϩ by a highly efficient Ca 2ϩ pump controlled by calmodulin (Lake et al, 1977;Cheung, 1980;Schrier et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

Temperature Transition of Human Hemoglobin at Body Temperature: Effects of Calcium

Kelemen

Chien

Artmann

2001

Biophysical Journal

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We studied the effects of calcium ion concentration on the temperature dependence of rheological behavior of human red blood cells (RBCs) and concentrated hemoglobin solutions. Our previous study (G. M. Artmann, C. Kelemen, D. Porst, G. Büldt, and S. Chien, 1998, Biophys. J., 75:3179-3183) showed a critical temperature (Tc) of 36.4 +/- 0.3 degrees C at which the RBCs underwent a transition from non-passage to passage through 1.3 microm micropipettes in response to an aspiration pressure of -2.3 kPa. An increase in intracellular Ca2+ concentration by using the ionophore A23187 reduced the passability of intact RBCs through small micropipettes above T(c); the micropipette diameter needed for >90% passage increased to 1.7 microm. Viscometry of concentrated hemoglobin solutions (45 and 50 g/dl) showed a sudden viscosity transition at 36 +/- 1 degrees C (Tc(eta)) at all calcium concentrations investigated. Below Tc(eta), the viscosity value of the concentrated hemoglobin solution at 1.8 mM Ca(2+) was higher than that at other concentrations (0.2 microM, 9 mM, and 18 mM). Above Tc(eta), the viscosity was almost Ca2+ independent. At 1.8 mM Ca2+ and 36 +/- 1 degrees C, the activation energy calculated from the viscometry data showed a strong dependence on the hemoglobin concentration. We propose that the transition of rheological behavior is attributable to a high-to-low viscosity transition mediated by a partial release of the hemoglobin-bound water.

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“…Transformation of erythrocyte morphology from discocyte to echinocyte can be initiated by increasing cytoplasmic Ca2+ concentration with the ionophore A23187 (1,4). This transformation is closely linked to intracellular ATP levels, which greatly decrease when external Ca2+ is translocated across erythrocyte membranes by the ionophore (1).…”

mentioning

confidence: 99%

Transformation of Human Erythrocyte Shape by Endotoxic Lipopolysaccharide

1983

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Human erythrocytes were observed to undergo a discocyte to echinocyte to spheroechinocyte shape transformation during brief incubation with endotoxic lipopolysaccharide. It was concluded that lipopolysaccharide-membrane interactions alter the curvature of erythrocyte membranes. Proc. Soc. Exp. Biol. Med. 161:255-259. 17. Warren,J. R. 1982. Polymyxin B suppresses the endotoxin inhibition of concanavallin A-mediated erythrocyte agglutination. Infect. Immun. 35:594-599. 18. Warren, J. R., and M. M. Kowalski. 1977. Inhibition of Con A erythroagglutination by alkali-treated lipopolysaccharide. Exp. Cell Res. 107:462-466. INFECT. IMMUN. on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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The effects of ionophore A23187 on erythrocytes relationship of ATP and 2,3-diphosphoglycerate to calcium-binding capacity

Cited by 18 publications

References 19 publications

Interaction of chemotactic factors with human macrophages: induction of transmembrane potential changes

Interaction of chemotactic factors with human macrophages: induction of transmembrane potential changes

Temperature Transition of Human Hemoglobin at Body Temperature: Effects of Calcium

Transformation of Human Erythrocyte Shape by Endotoxic Lipopolysaccharide

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