The effects of intrahippocampal raphe and/or septal grafts in rats with fimbria-fornix lesions depend on the origin of the grafted tissue and the behavioural task used

Jeltsch, Hélène; Cassel, J.C.; Neufang, B.; Kelche, Christian; Hertting, G.; Jackisch, Rolf; Will, Bruno

doi:10.1016/0306-4522(94)90004-3

Cited by 39 publications

(52 citation statements)

References 60 publications

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“…The latter observation (confirmed in other studies; e.g. Cassel et al, 1993b;Jeltsch et al, 1994b), demonstrates that, when grafts are implanted in the dorsal half of the hippocampus, there always exists a decreasing septo-temporal gradient of the graft-induced effects on the cholinergic markers. That this graft-induced increase in ChAT activity actually reflected increased cholinergic function was confirmed in the same study by the determination of [14C]acetylcholine synthesis from ['4C]glucose (recovery to 94°/0 of normal; lesion effect: 60°A decrease).…”

Section: Intraparenchymal Injections Of Cell Suspensionssupporting

confidence: 75%

“…Interestingly, many of the behavioral deficits exhibited by rats that sustained intraventricular or intraparenchymal injections of AF64A actually resemble those reported after more or less extensive, nut non-specific lesions of the septal region or the fimbria, the dorsal fornix and the cingular bundle (e.g. Collerton, 1986;Dunnett, 1990;Jeltsch et al, 1994aJeltsch et al, , 1994b, and references in Section 3.3.2.1). These rats show increased levels of activity in both a familiar and an unfamiliar environment, impaired performance in passive and active avoidance tests, as well as severely perturbated capabilities of T-maze, radial maze and Morris water maze learning (e.g.…”

Section: Cholinergic Denervation Paradigmsmentioning

confidence: 81%

“…Daszuta et al, 1988; see also Auerbach et al, 1985). Aspiration lesions of the supracallosal and infracallosal pathways were found to increase this ratio in the dorsal hippocampus to about 400~0 of normal (Cassel et al, 1993b) and electrolytic lesions restricted to the infracallosal pathways to about 2500/0 (Jeltsch et al, 1994b). As regards the status of the 5-HT receptors in the denervated hippocampus, previous studies have reported the density of the hippocampal 5-HT, receptors (with no distinction of the subtype) to be reduced by approximately 500/0after fimbria-fornix lesions (Quirion and Richard, 1987) and the binding to non-5 -HT,~sites to be reduced by 20% after septal lesions (Kiedrowski et al, 1990).…”

Section: Serotonergic Systemmentioning

confidence: 92%

“…an open field) environments (e.g. Capobianco and Hamilton, 1976;Cassel et al, 1991b;Dunnett et al, 1982;Jeltsch et al, 1994aJeltsch et al, , 1994b. Hippocampal lesions also attenuate the cyclic activity changes (Jarrard, 1968).…”

Section: Main~enance %Ehavioursmentioning

confidence: 99%

See 3 more Smart Citations

The Fimbria-Fornix/Cingular Bundle Pathways: A Review of Neurochemical and Behavioural Approaches Using Lesions and Transplantation Techniques

Cassel

Duconseille

Jeltsch

et al. 1997

Progress in Neurobiology

103

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Section: Intraparenchymal Injections Of Cell Suspensionssupporting

confidence: 75%

Section: Cholinergic Denervation Paradigmsmentioning

confidence: 81%

Section: Serotonergic Systemmentioning

confidence: 92%

Section: Main~enance %Ehavioursmentioning

confidence: 99%

See 2 more Smart Citations

The Fimbria-Fornix/Cingular Bundle Pathways: A Review of Neurochemical and Behavioural Approaches Using Lesions and Transplantation Techniques

Cassel

Duconseille

Jeltsch

et al. 1997

Progress in Neurobiology

103

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“…in rats with fimbria-fornix lesions and intrahippocampal fetal septal cell suspension grafts. The electrolytic fimbria-fornix lesion paradigm was chosen because in previous studies we found intrahippocampal grafts of fetal septal cell suspensions to have no beneficial effect on lesion-induced radial-arm maze (RAM) learning deficits in rats (Hofferer et al 1996;Jeltsch et al 1994b). After 2 months of GM1 treatment, the behavioral effects of the lesions, grafts, and GM1 injections were assessed using tests evaluating home-cage locomotor activity, open-field (re)activity and eight-arm radial maze performance with both an uninterrupted and an interrupted testing procedure.…”

Section: Introductionmentioning

confidence: 99%

Behavioral effects of GM1 ganglioside treatment and intrahippocampal septal grafts in rats with fimbria-fornix lesions

1997

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The monosialoganglioside GM1 is a compound with neurotrophic properties found to foster functional recovery in various paradigms of brain damage. The present experiment examined whether systemic treatment with GM1 may facilitate behavioral recovery in rats with fimbria-fornix lesions and intrahippocampal grafts rich in cholinergic neurons. Among 68 Long-Evans female rats, 46 sustained a bilateral electrolytic lesion of the fimbria and the dorsal fornix and 22 were sham-operated. Fourteen days later, half the lesioned rats were subjected to intrahippocampal grafts of a fetal septal cell suspension. Starting a few hours after lesion surgery and over a 2-month period, half the rats of each surgical treatment group received a daily injection of GM1 (30 mg/kg i.p.), the other half being injected with saline as a control. All rats were subsequently tested for locomotor activity and radial maze learning. The lesions induced locomotor hyperactivity and impaired learning performances in both an uninterrupted and an interrupted radial maze testing procedure. In all rats with surviving grafts, the grafts had provided the hippocampus with a new and dense organotypic acetylcholinesterase-positive innervation pattern which did not differ between saline- and GM1-treated subjects. The scores/performances of the rats that had received only the grafts or only the GM1 treatment did not differ significantly from those of their respective lesion-only counterparts. However, in the radial-arm maze task, the grafted rats given GM1 showed improved learning performances as compared with their saline-treated counterparts: they used more efficient visit patterns under the uninterrupted testing conditions and made fewer errors under the interrupted ones. The results suggest that GM1 treatment or intrahippocampal grafts used separately do not attenuate the lesion-induced behavioral deficits measured in this experiment. However, when GM1 treatment and grafts are used conjointly, both may interact in a manner allowing part of these deficits to be attenuated.

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Up-regulation of genes involved in cellular stress and apoptosis in a rat model of hippocampal degeneration

Anguelova¹,

Boularand²,

Nowicki³

et al. 2000

Journal of Neuroscience Research

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Changes in gene expression within the hippocampus induced by denervation after electrolytic fimbria-fornix lesion in rat were compared to morphological and biochemical alterations. Fimbria-fornix lesion results in degeneration of hippocampal cholinergic terminals as evidenced by a sustained (2 days to 1 month) decrease in cholineacetyltransferase (ChAT) activity (50%). These changes were accompanied by a decrease in growth associated protein 43 (GAP-43) immunoreactivity in all hippocampal layers 4 days after lesion followed by a subsequent increase and return to normal levels by 20 days postinjury. This increase in GAP-43 expression in the hippocampus between 7 to 20 days after lesion may reflect heterotypic sprouting. TUNEL-positive cells were revealed by in situ assay within the hippocampus at 10 days, but not at 3 days, after lesion. Two subtracted cDNA libraries from the dorsal hippocampus of control and injured rats (at 3 and 10 days postlesion) were constructed in order to search for new genes potentially implicated in degeneration/regeneration phenomena. We analysed 1,536 clones from each library by differential screening and found a total of 46 up-regulated genes. Among the 15 known genes, 6 coded for proteins involved in signal transduction pathways. The upregulation of growth arrest DNA damage induced gene (GADD153), brain-specific RING finger protein, JNK interacting protein (JIP-1), protein kinase A (PKA), and Na+K+ ATPase was studied by quantitative polymerase chain reaction (PCR). Two of these genes, GADD153 and JIP-1, have been previously shown to participate in cell modifications induced by stress and apoptosis.

show abstract

The effects of intrahippocampal raphe and/or septal grafts in rats with fimbria-fornix lesions depend on the origin of the grafted tissue and the behavioural task used

Cited by 39 publications

References 60 publications

The Fimbria-Fornix/Cingular Bundle Pathways: A Review of Neurochemical and Behavioural Approaches Using Lesions and Transplantation Techniques

The Fimbria-Fornix/Cingular Bundle Pathways: A Review of Neurochemical and Behavioural Approaches Using Lesions and Transplantation Techniques

Behavioral effects of GM1 ganglioside treatment and intrahippocampal septal grafts in rats with fimbria-fornix lesions

Up-regulation of genes involved in cellular stress and apoptosis in a rat model of hippocampal degeneration

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