The Effects of Interferon on Measles Virus Rna Synthesis

Milstien, Julie B.; Seifried, A S; Klutch, Michael; Bhatia, B.

doi:10.1016/b978-0-12-102480-2.50031-3

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…The effects of IFN on MV replication at the molecular level are poorly characterized. However, an inhibition of MV genomic RNA synthesis in PBMC caused by the addition of exogenous IFN has been described (17). Also the biological significance of the observed inhibitory effect on MV replication by IFN, i.e.…”

Section: Replication Of Vesicular Stomatitis Virus (Vsv)mentioning

confidence: 99%

Effect of interferon‐α on measles virus replication in human peripheral blood mononuclear cells

Leopardi

Hyypiä

Vainionpää

1992

APMIS

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Leopardi, R., Hyypia, T. & Vainionpaa, R. Effect of interferon-a on measles virus replication in human peripheral blood mononuclear cells. APMIS 100: 125-1 3 1, 1992.We analyzed the effect of exogenous human leukocyte interferon (1FN)-a on measles virus (MV) replication in human peripheral blood mononuclear cells (PBMC). The release of infectious virus was progressively reduced by increasing concentrations of IFN-a, and blocked with an IFN-a concentration of 1000 U/ml. In order to detect a possible target of this inhibitory effect, viral transcription and translation events were analyzed. The synthesis of MV mRNAs was reduced, but not blocked, in the presence of IFN-a. However, this effect was not specific on the viral RNAs, but due to a general inhibition of RNA synthesis in IFN-treated PBMC. The expression of viral polypeptides was also inhibited in a dose-dependent manner by exogenous IFN-a, but a low level of protein synthesis was detected by both Western blotting and immunofluorescence techniques, even with the maximum amount of IFN-a used (1000 U/ml). These findings account for a partial maintenance of the viral replicative cycle, even when the production of infectious virus is blocked. Moreover, the effect of IFN-a is not specifically targeted on the virus macromolecular synthesis.

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Section: Replication Of Vesicular Stomatitis Virus (Vsv)mentioning

confidence: 99%

Effect of interferon‐α on measles virus replication in human peripheral blood mononuclear cells

Leopardi

Hyypiä

Vainionpää

1992

APMIS

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Identification of a nonproductive, cell-associated form of measles virus by its resistance to inhibition by recombinant human interferon

Carrigan¹,

Kabacoff²

1987

J Virol

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Subacute sclerosing panencephalitis (SSPE) is a fatal disease in children and young adults that is caused by persistent infection of the central nervous system (CNS) by a nonproductive, cell-associated form of measles virus. Using an experimental model for SSPE (LEC viral strain in newborn hamsters), we have shown previously that establishment of such CNS infections involves selective elimination from the CNS of productively infected cells by host defensive mechanisms, coupled with the selective sparing of cells carrying nonproductive viral forms. That interferon (IFN) may play a role in this process was suggested by the disappearance of productively infected cells from the CNS tissues prior to the appearance of antiviral antibodies and by the demonstration of cell-associated, IFN-resistant viral variants in the virus stocks that were used. Results of this study support these conclusions by showing that similar IFN-resistant viral variants are present in the HBS strain of SSPE-derived measles virus and that these variants, in the presence of IFN, have properties that are similar to those of naturally occurring cell-associated'strains of SSPE viruses, e.g., DR, IP3, and Biken. These IFN-resistant forms of HBS virus were isolated and were shown to maintain their resistance to inhibition by IFN after cloning. However, on removal of IFN, they reverted to productive forms similar to the parental HBS virus. The potential role of such viral forms in the pathogenesis of SSPE is discussed.

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The Effects of Interferon on Measles Virus Rna Synthesis

Cited by 2 publications

References 10 publications

Effect of interferon‐α on measles virus replication in human peripheral blood mononuclear cells

Effect of interferon‐α on measles virus replication in human peripheral blood mononuclear cells

Identification of a nonproductive, cell-associated form of measles virus by its resistance to inhibition by recombinant human interferon

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