The effects of interactive mechanical and biochemical niche signaling on osteogenic differentiation of adipose-derived stem cells using combinatorial hydrogels

Nii, Michelle; Lai, Janice H.; Keeney, Michael; Han, Li‐Hsin; Behn, Anthony W.; Imanbayev, Galym; Yang, Fan

doi:10.1016/j.actbio.2012.11.002

Cited by 71 publications

(51 citation statements)

References 35 publications

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“…In recent years, several studies have attempted to fully exploit the modular possibilities of synthetic materials to systematically deconstruct the in vivo niche using high-throughput approaches (12,13). Although these studies have heralded the potential of robotically arrayed, rationally designed 3D microenvironmental manipulation of the extracellular microenvironment, they have not tackled the problem of multicellular morphogenesis.…”

mentioning

confidence: 99%

Neural tube morphogenesis in synthetic 3D microenvironments

Ranga

Girgin

Meinhardt

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

196

169

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Three-dimensional organoid constructs serve as increasingly widespread in vitro models for development and disease modeling. Current approaches to recreate morphogenetic processes in vitro rely on poorly controllable and ill-defined matrices, thereby largely overlooking the contribution of biochemical and biophysical extracellular matrix (ECM) factors in promoting multicellular growth and reorganization. Here, we show how defined synthetic matrices can be used to explore the role of the ECM in the development of complex 3D neuroepithelial cysts that recapitulate key steps in early neurogenesis. We demonstrate how key ECM parameters are involved in specifying cytoskeleton-mediated symmetry-breaking events that ultimately lead to neural tube-like patterning along the dorsal–ventral (DV) axis. Such synthetic materials serve as valuable tools for studying the discrete action of extrinsic factors in organogenesis, and allow for the discovery of relationships between cytoskeletal mechanobiology and morphogenesis.

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mentioning

confidence: 99%

Neural tube morphogenesis in synthetic 3D microenvironments

Ranga

Girgin

Meinhardt

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

196

169

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“…In anisotropic scaffolds for skeletal muscle tissue engineering, biochemical cues may be intrinsic to the scaffold base material as is the case for natural polymers, that present biochemical cues via functional surface groups. [61] Otherwise, to mimic native ECM, anisotropic scaffold surface functionality can be tailored via physical or covalent adsorption of biological molecules such as heparin and/or fibronectin or via other modifications [18, 62] to promote cell-surface interactions via integrins and subsequent induction of intracellular signaling pathways. [63] Functional groups inherent to various polymers, such as chitosan, are also useful for conjugation of additional biochemical moieties.…”

Section: Skeletal Muscle Tissue Engineering Approachesmentioning

confidence: 99%

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

2016

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Repair of damaged skeletal muscle tissue is limited by the regenerative capacity of native tissue. Current clinical approaches are not optimal for treatment of large volumetric skeletal muscle loss. As an alternative, tissue engineering represents a promising approach for the functional restoration of damaged muscle tissue. A typical tissue engineering process involves the design and fabrication of a scaffold that closely mimics the native skeletal muscle extracellular matrix allowing for organization of cells into a physiologically relevant, 3D architecture. In particular, anisotropic materials, which mimic the morphology of the native skeletal muscle ECM, can be fabricated using various biocompatible materialsto guide cell alignment, elongation, proliferation and differentiation into myotubes. In this article, we first provide an overview of fundamental concepts associated with muscle tissue engineering and the current status of the muscle tissue engineering approaches. We then review recent advances in development of anisotropic scaffolds with micro- or nano-scale features and examine how scaffold topographical, mechanical, and biochemical cues correlate to observed cellular function and phenotype development. Finally, we highlight some recent developments in both the design and utility of anisotropic materials in skeletal muscle tissue engineering along with their potential impact on future research and clinical application.

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“…From these seminal studies, investigators have examined other types of physical stimuli such as combinations of different mechanical or physicochemical loading regimens that could even further improve construct qualities (Huang et al, 2012; Huey and Athanasiou, 2011; Jungreuthmayer et al, 2009; Meinel et al, 2004; Sampat et al, 2013; Schatti et al, 2011; Zhang et al, 2009). With further understanding of the role of the biomechanical microenvironment on cell behavior (Guilak et al, 2009; Reilly and Engler, 2010), other studies have examined the role of factors such as extracellular matrix topography or modulus within scaffolds as means of influencing local cellular responses (Dellatore et al, 2008; Gauvin et al, 2012; Guex et al, 2013; Kolambkar et al, 2014; Lim et al, 2011; Nii et al, 2013). …”

Section: Principles Of Functional Tissue Engineeringmentioning

confidence: 99%

Biomechanics and mechanobiology in functional tissue engineering

Guilak

Butler

Goldstein

et al. 2014

Journal of Biomechanics

195

127

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The field of tissue engineering continues to expand and mature, and several products are now in clinical use, with numerous other preclinical and clinical studies underway. However, specific challenges still remain in the repair or regeneration of tissues that serve a predominantly biomechanical function. Furthermore, it is now clear that mechanobiological interactions between cells and scaffolds can critically influence cell behavior, even in tissues and organs that do not serve an overt biomechanical role. Over the past decade, the field of “functional tissue engineering” has grown as a subfield of tissue engineering to address the challenges and questions on the role of biomechanics and mechanobiology in tissue engineering. Originally posed as a set of principles and guidelines for engineering of load-bearing tissues, functional tissue engineering has grown to encompass several related areas that have proven to have important implications for tissue repair and regeneration. These topics include measurement and modeling of the in vivo biomechanical environment; quantitative analysis of the mechanical properties of native tissues, scaffolds, and repair tissues; development of rationale criteria for the design and assessment of engineered tissues; investigation of the effects biomechanical factors on native and repair tissues, in vivo and in vitro; and development and application of computational models of tissue growth and remodeling. Here we further expand this paradigm and provide examples of the numerous advances in the field over the past decade. Consideration of these principles in the design process will hopefully improve the safety, efficacy, and overall success of engineered tissue replacements.

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The effects of interactive mechanical and biochemical niche signaling on osteogenic differentiation of adipose-derived stem cells using combinatorial hydrogels

Cited by 71 publications

References 35 publications

Neural tube morphogenesis in synthetic 3D microenvironments

Neural tube morphogenesis in synthetic 3D microenvironments

Anisotropic Materials for Skeletal‐Muscle‐Tissue Engineering

Biomechanics and mechanobiology in functional tissue engineering

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