The Effects of Insulin-Like Growth Factor-1 Gene Therapy and Cell Transplantation on Rat Acute Wound Model

Amiri, Fereshteh Talebpour; Fathabadi, Fatemeh Fadaei; Rad, Mahnaz Mahmoudi; piryae, Abbas; Ghasemi, Azar; Khalilian, A R; Yeganeh, Farshid; Mosaffa, Nariman

doi:10.5812/ircmj.16323

Cited by 13 publications

(5 citation statements)

References 35 publications

(43 reference statements)

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“…e IGF family is expected to have an essential role in regulating cell proliferation, apoptosis, and transformation [11]. IGF1, widely regarded as a circulating growth factor, is normally produced by the liver and participates in mediating body growth through growth hormone e ects [40], but it is fatal for the normal development and growth of cell maintenance and homeostasis [9].…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis of the association of IGFBP3 and IGF1 polymorphisms with susceptibility to colorectal cancer

Wang¹,

Bu-qiang²,

Chen³

et al. 2018

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e aim of this study is to comprehensively evaluate the associations of IGFBP3 and IGF1 polymorphisms with susceptibility to colorectal cancer (CRC). We searched the English and Chinese databases and recruited case-control studies based on strict inclusion and exclusion criteria. e statistical analysis was performed by the Comprehensive Metaanalysis 2.0 (CMA 2.0) so ware and this initially identi ed 251 studies. We then recruited 10 English studies to this metaanalysis detailed review which includes 9,415 CRC patients and 14,179 healthy controls. Our results demonstrated that IGFBP3 rs2854746 C>G polymorphism increases susceptibility to the CRC (allele model: OR=1.167, 95% CI=1.095~1.244, p<0.001 and to the dominant gene model: OR=1.226, 95% CI=1.113~1.350, p<0.001); but IGFBP3 rs2854744 A>C has no signi cant association with the CRC susceptibility (allele model: OR=0.970, 95% CI=0.932~1.010, p=0.138; dominant gene model: OR=0.995, 95% CI=0.936~1.057, p=0.874). Also, IGF1 rs35767 C>T polymorphism decreases susceptibility to CRC (allele model: OR=0.785, 95% CI=0.726~0.850, p<0.001 and also the dominant model: OR=0.730, 95% CI=0.661~0.806, p<0.001). However, IGFBP3 rs2854746 C>G is considered the susceptible CRC polymorphism and IGF1 rs35767 C>T is CRC protective.

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Section: Discussionmentioning

confidence: 99%

Meta-analysis of the association of IGFBP3 and IGF1 polymorphisms with susceptibility to colorectal cancer

Wang¹,

Bu-qiang²,

Chen³

et al. 2018

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“…Thus, our results indicated that wounded skin treated with EFA had higher local IGF-1 mRNA expression throughout the experiment, which may benefit the wound healing outcome. Gene therapy with the IGF-1 gene transferred to fibroblasts was found to upregulate the expression of IGF-1 and improve wound healing outcome [33].…”

Section: Discussionmentioning

confidence: 99%

Topical essential fatty acid oil on wounds: Local and systemic effects

et al. 2019

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BackgroundThe use of medicinal plants and their derivatives is increasing, and approximately one-third of all traditional herbal medicines are intended for wound treatment. Natural products used in these treatments include vegetable oils, which are rich in essential fatty acids. Once in contact with an ulcerative surface, the oil reaches the blood and lymphatic vessels, thus eliciting systemic effects.ObjectiveThis study evaluated the local and possible systemic effects of essential fatty acids (sunflower oil) applied topically to rat wounds.MethodsCutaneous punch wounds (6 mm) were produced on the dorsa of 30 rats. Saline (SS), mineral oil (MO) or essential fatty acid (EFA) solutions were applied topically. Healing was evaluated after 2, 4 and 10 days (n = 5 per group) by visual and histological/morphometric examination, second harmonic generation (SHG) microscopy, and cytokine and growth factor quantification in the scar tissue (real-time PCR) and in serum (ELISA).ResultsMO/EFA-treated animals had higher IGF-1, leptin, IL-6 and IFN-γ mRNA expression and lower serum IL-6 levels than the control (SS/MO) animals. SHG analysis showed no difference in collagen density between the animals treated with MO and EFA.ConclusionEFA treatment induces topical (observed by local IGF-1, leptin, IL-6 and IFN-γ production) and systemic effects, lowering IL-6 levels in the serum. As the oil is widely used to shorten ulcer healing time, studies are needed to evaluate the treatment safety and possible undesired effects.

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“…IGF-1 is a growth factor produced by fibroblasts and other epithelial cells, and it plays an important role in re-epithelialization and granulation tissue formation during the wound healing process [ 32 ]. One of its roles is vascular endothelial insulin/IGF-1 signaling in the homeostasis of the skin vasculature and in neovascularization on the skin during wound healing [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)

et al. 2017

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IntroductionWounds are a common health problem. Coffee is widely consumed and its oil contains essential fatty acids. We evaluated the local (skin) and systemic effects associated with the topical use of coffee oils in rats.MethodsPunch skin wounds (6 mm) incisions were generated on the backs of 75 rats. Saline (SS), mineral oil (MO), green coffee oil (GCO), roasted coffee oil (RCO), green coffee ground oil (GCGO) or roasted coffee ground oil (RCGO) were topically applied to the wounds. Healing was evaluated by visual and histological/morphometric optical microscopy examination; second harmonics generation (SHG) microscopy, wound tissue q-PCR (values in fold-change) and blood serum (ELISA, values in pg/mL).ResultsRCO treated animals presented faster wound healing (0.986 vs. 0.422), higher mRNA expression of IGF-1 (2.78 vs. 1.00, p = 0.01), IL-6 (10.72 vs. 1.00, p = 0.001) and IL-23 (4.10 vs. 1.2, p = 0.05) in early stages of wound healing; higher IL-12 (3.32 vs. 1.00, p = 0.05) in the later stages; and lower serum levels of IFN-γ (11.97 vs. 196.45, p = 0.01). GCO treatment led to higher mRNA expression of IL-6 (day 2: 7.94 vs. 1.00, p = 0.001 and day 4: 6.90 vs. 1.00, p = 0.01) and IL-23 (7.93 vs. 1.20, p = 0.001) in the early stages. The RCO treatment also produced higher serum IFN-α levels throughout the experiment (day 2: 52.53 vs. 21.20; day 4: 46.98 vs.21.56; day 10: 83.61 vs. 25.69, p = 0.05) and lower levels of IL-4 (day 4: 0.9 vs.13.36, p = 0.01), adiponectin (day 10: 8,367.47 vs. 16,526.38, p = 0.001) and IFN-γ (day 4: 43.03 vs.196.45, p = 0.05). The SHG analysis showed a higher collagen density in the RCO and GCO treatments (p = 0.05).ConclusionTopical treatment with coffee oils led to systemic actions and faster wound healing in rats. Further studies should be performed are necessary to assess the safety of topical vegetal oil use for skin lesions.

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The Effects of Insulin-Like Growth Factor-1 Gene Therapy and Cell Transplantation on Rat Acute Wound Model

Cited by 13 publications

References 35 publications

Meta-analysis of the association of IGFBP3 and IGF1 polymorphisms with susceptibility to colorectal cancer

Meta-analysis of the association of IGFBP3 and IGF1 polymorphisms with susceptibility to colorectal cancer

Topical essential fatty acid oil on wounds: Local and systemic effects

Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)

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