The Effects of Insulin and Follicle-Simulating Hormone (FSH) During In Vitro Development of Ovarian Goat Preantral Follicles and the Relative mRNA Expression for Insulin and FSH Receptors and Cytochrome P450 Aromatase in Cultured Follicles1

Chaves, Roberta Nogueira; Duarte, A.B.G.; Rodrigues, Giovanna Quintino; Celestino, J.J.H.; Silva, Gerlane M.; Lopes, C.A.P.; Almeida, A. P.; Donato, Mariana Aragão Matos; Peixoto, Christina Alves; Moura, Arlindo A.; Lobo, Carlos Henrique; Locatelli, Yann; Mermillod, Pascal; Campello, C.C.; Figueiredo, J.R.

doi:10.1095/biolreprod.112.099010

Cited by 51 publications

(38 citation statements)

References 43 publications

(52 reference statements)

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“…Several studies have been developed by our team in an attempt to improve oocyte growth rates, resumption of meiosis, and maturation from in vitro‐cultured caprine preantral follicles, but with limited success (Araújo et al, ; Chaves et al, ). The rates of oocyte maturation are still low even with the addition of hormones and/or growth factors to the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

Brito

Silva

Duarte

et al. 2014

Molecular Reproduction Devel

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This study examined caprine follicular development in different concentrations of alginate matrix to determine the optimal conditions for culture. Caprine preantral follicles were cultured in a two-dimensional system (control) or a three-dimensional encapsulated system in 0.25%, 0.5%, or 1% alginate (ALG 0.25, ALG 0.5, and ALG 1, respectively). A higher percentage of morphologically normal follicles developed in ALG 0.5 and ALG 1 than in ALG 0.25 or the control (P < 0.05). The rate of antrum formation, however, was higher in ALG 0.25 than in ALG 0.5 and ALG 1 conditions (P < 0.05), but similar to the control. Follicles cultured in ALG 0.25 had higher growth rates and meiotic resumption than those cultured in ALG 0.5, ALG 1, or the control (P < 0.05). Moreover, follicles cultured in ALG 0.25 had higher levels of estradiol and progesterone than those cultured in ALG 0.5, ALG 1, or the control, as well as higher levels of CYP19A1 and HSD3B mRNA. In conclusion, a three-dimensional system that uses ALG 0.25 fosters the in vitro development of caprine preantral follicles and increases the rate of meiotic resumption.

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Section: Discussionmentioning

confidence: 99%

Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

Brito

Silva

Duarte

et al. 2014

Molecular Reproduction Devel

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“…Most studies reporting the effects of AMH on steroid secretion used granulosa-luteal cells in monolayer culture, which may not reflect the effects of AMH on intact follicles because of the well-known importance of oocytegranulosa cell cross-talk in follicular physiology (Diaz et al 2007). In contrast with the present study, Chaves et al (2012) reported that the addition of rbFSH (Days 0-6: 100 ng mL À1 ; Days 6-12: 500 ng mL À1 ; Days 12-18: 1000 ng mL À1 ) to the culture medium containing a low insulin concentration significantly increased E2 production by isolated caprine preantral follicles cultured in vitro. This discrepancy may be due to the concentration of FSH used (fixed vs increasing).…”

Section: Discussionmentioning

confidence: 58%

“…Rodrigues et al (2014) showed that FSH combined with a low concentration of insulin (10 ng mL À1 ) did not improve the survival of isolated caprine secondary follicles. In contrast with the follicular growth endpoint, studies in goats have shown that high rates of follicular survival are obtained even using a very simple medium without the addition of hormones or growth factors (Chaves et al 2012). In the present study, 50 ng mL À1 AMH reduced follicular growth (speed of development) without affecting meiotic resumption.…”

Section: Discussionmentioning

confidence: 99%

Anti-Müllerian hormone reduces growth rate without altering follicular survival in isolated caprine preantral follicles cultured in vitro

Rocha

Lima

Brito

et al. 2017

Reprod. Fertil. Dev.

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The aim of the present study was to evaluate the effect of anti-Müllerian hormone (AMH), with and without FSH, on the in vitro development of isolated caprine preantral follicles, as well as follicular steroid production and mRNA levels of AMH, hormone receptors (AMH and FSH), CYP19A1 (cytochrome P450, family 19, subfamily A, polypeptide 1), CYP17 (cytochrome P450, family 17, subfamily A, polypeptide 1), HSD3B (3-beta-hydroxysteroid dehydrogenase) and Myc (myelocytomatosis oncogene). Isolated secondary follicles were cultured in minimum essential medium alpha (α-MEM+) alone or supplemented with 50ng mL AMH and/or 100ng mL FSH added sequentially on different days of culture. Follicles were cultured for a total of 18 days, with different media during the first (Days 0-9) and second (Days 10-18) halves of the culture period, resulting in six treatment groups, as follows: α-MEM+/α-MEM+, FSH/FSH, AMH/AMH, AMH+FSH/AMH+FSH, AMH/FSH, and FSH/AMH. Follicle development was evaluated on the basis of follicular growth, oocyte maturation and steroid secretion. There was a decrease in follicular growth rate in the AMH, AMH+FSH and AMH/FSH treatment groups compared with α-MEM+ and FSH treatment groups (P<0.05). However, the different culture conditions had no effect on rates of meiotic resumption and steroid secretion (P>0.05). Moreover, follicles cultured in the presence of FSH had lower levels of AMH receptor type II (AMHRII) mRNA compared with non-cultured control (freshly isolated follicles), and the AMH and AMH/FSH treatment groups. In conclusion, AMH reduces the follicular growth rate of isolated goat preantral follicles in vitro without affecting follicular survival.

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“…It also facilitates FSH-dependent steroid production and LH receptor induction in cultured granulosa cells (DULEBA et al, 1997;MAY et al, 1980). This combination of hormones also stimulated follicular development and maintained follicular survival, as well as increased estradiol secretion and promoted oocyte meiosis resumption in goat preantral follicles (CHAVES et al, 2012).…”

Section: Discussionmentioning

confidence: 97%

Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium

Gonçalves

Cavalcante

Gouveia

et al. 2017

AVB

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Article historyThis study evaluated the effect of caprine ovarian tissue transportation conditions (medium supplementation and transportation duration) on the morphology, DNA fragmentation and development of cultured and non-cultured preantral follicles. After the fragmentation of ovaries, one fragment was fixed (fresh control) while the remaining slices were placed individually in two different conservation media (Minimal Essential Medium -MEM without supplementation or supplemented MEM, i.e. MEM + ) and stored at 35ºC for 6 or 12 h without (non-cultured) or with a subsequent 5-day in vitro culture in supplemented α-MEM. After transportation, followed or not by in vitro culture, the fragments were processed for histological and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) examination. For the preserved and non-cultured fragments, the percentages of normal follicles after the storage of ovarian tissue in MEM + for 6 h and the DNA fragmentation rates after preservation in MEM for 6 h and MEM + for 6 or 12 h were maintained similar to the fresh control. However, all cultured treatments reduced the proportion of normal follicles and increased the percentage of TUNEL-positive cells as compared to the fresh control and non-cultured treatments. On the contrary, all culture conditions (except after preservation in MEM for 6 h) promoted an increase in primordial follicle activation. In conclusion, the use of an enriched medium (MEM + ) during ovary transportation is preferable to maintain satisfactory rates of normal follicles after the preservation of caprine ovarian tissue at 35ºC for up to 6 h, without affecting the ability of the primordial follicle to grow in vitro.

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The Effects of Insulin and Follicle-Simulating Hormone (FSH) During In Vitro Development of Ovarian Goat Preantral Follicles and the Relative mRNA Expression for Insulin and FSH Receptors and Cytochrome P450 Aromatase in Cultured Follicles1

Cited by 51 publications

References 43 publications

Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

Alginate hydrogel matrix stiffness influences the in vitro development of caprine preantral follicles

Anti-Müllerian hormone reduces growth rate without altering follicular survival in isolated caprine preantral follicles cultured in vitro

Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium

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