The effects of incisional myringotomy and CO2 laser myringotomy on rat tympanic membranes

Poyrazoğlu, Ethem; Cincik, Hakan; Güngör, Atila; Gürpınar, Berk; Yıldırım, Şükrü; Candan, Hasan

doi:10.1016/j.ijporl.2004.01.018

Cited by 7 publications

(2 citation statements)

References 10 publications

(21 reference statements)

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“…An extension of our study may be to look at the biological effects of a more extended duration of ventilation. This could be achieved by laser myringotomy [47][48][49][50][51][52][53][54] or by application of mitomycin-C 55-59 to the incision, but these methods probably only slightly prolong patency of myringotomy and may in themselves contribute to inflammation.…”

Section: Discussionmentioning

confidence: 99%

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

2014

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Objectives/Hypothesis: Ventilation of the chronically inflamed middle ear is a key outcome in functional middle ear surgery. Grommets eliminate middle ear effusion, but there is also evidence that they downregulate inflammation. The reason for this is not understood, but there is little to suggest alteration in eustachian tube ventilatory capacity. Previous work has shown that the Junbo mouse model of chronic otitis media has hypoxic middle ear mucosa and bulla fluid leucocytes. Here we explore whether surgical ventilation may alleviate chronic otitis media through downregulation of hypoxia.Study Design: Surgical intervention on a mouse model of disease. Methods: We established patency of myringotomy incision as 5 days in wild-type mice. We performed unilateral myringotomy on three cohorts of mice: 10 wild-type controls, 12 Junbo mice, and 15 Junbo mice with additional removal of middle ear effusion. A small cohort of these mice were labeled in vivo by intraperitoneal injection of pimonidazole to identify tissue hypoxia. Tissues were assessed for mucoperiosteal thickening and pimonidazole labeling, comparing operated to nonoperated ears.Results: Ventilation of the inflamed Junbo middle ear revealed significant reduction in inflammatory thickening associated with loss of pimonidazole labeling, suggesting resolution of cellular hypoxia.Conclusions: Surgical ventilation may achieve therapeutic effect through alleviation of cellular hypoxia in the chronically inflamed middle ear. Targeted molecular therapy of hypoxia signaling may offer future alternative therapy for chronic otitis media.

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Section: Discussionmentioning

confidence: 99%

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

2014

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“…Along with the literature, we think that MMC is superior to laser and thermal myringotomy. CO 2 laser myringotomy experience in our clinic on rats showed that it may prolong myringotomy patency time at least 2.5 weeks (8). Ragab (5) and Jasir et al (6) found that the dose of 0.4 mg/mL MMC is almost the optimal dose, and the novel study used the same dose in patients.…”

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confidence: 98%

Effects of Topical Application of Mitomycin-C and 5-Fluorouracil on Myringotomy in Rats

Cekin

Cincik

2006

Otology &Amp; Neurotology

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Re-evaluation of CO2 laser myringotomy for managing children with persistent acute otitis media

Kuo

Wang

Chu

et al. 2011

Journal of the Chinese Medical Association

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The effects of incisional myringotomy and CO2 laser myringotomy on rat tympanic membranes

Cited by 7 publications

References 10 publications

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

Effects of Topical Application of Mitomycin-C and 5-Fluorouracil on Myringotomy in Rats

Re-evaluation of CO2 laser myringotomy for managing children with persistent acute otitis media

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