The effects of iloprost on lung injury induced by skeletal muscle ischemia–reperfusion

Abstract

Purpose: The aim of this study was to investigate the effects of iloprost (I) on lung injury as a remote organ following skeletal muscle ischemia-reperfusion injury in a rat model. Materials and methods: Twenty-four Wistar Albino rats were randomized into four groups (n = 6). Laparotomy was performed in all groups under general anesthesia. Only laparotomy was applied in Group S (Sham). Ischemia reperfusion group (Group I/R) underwent ischemia and reperfusion performed by clamping and declamping of the infrarenal abdominal aorta for 120 minutes. Group iloprost (Group I) received intravenous infusion of iloprost 0.5 ng/kg/min, without ischemia and reperfusion. Group I/R/I received intravenous infusion of iloprost 0.5 ng/kg/min immediately after 2 hours of ischemia. At the end of the study, lung tissue was obtained for determining total oxidant status (TOS) and total antioxidant status (TAS) levels, histochemical and immunohistochemical determination. Results: Diffuse lymphocyte infi ltration was detected in immunohistochemical examination of lung tissue in Group I/R. The connective tissue around bronchi, bronchioles and vessel walls was found to be increased. Although minimal local lymphocyte infi ltration was detected in some fi elds in Group I/R/I, the overall tissue was found to be similar to Group S. iNOS expression was signifi cantly higher in Group I/R, when compared with Group S and signifi cantly lower in Group I/R/I compared to Group I/R. TOS levels were signifi cantly higher in Group I/R, when compared with groups S and I (p = 0.028, p = 0.016, respectively) and signifi cantly lower in group I/R/I, when compared with Group I/R (p = 0.048). TAS levels were signifi cantly higher in Group I/R, when compared with groups S, I (p = 0.014, p = 0.027, respectively) and signifi cantly lower in Group I/R/I, when compared with Group I/R (p = 0.032). Conclusion: These results indicate that administration of iloprost may have protective effects against ischemia reperfusion injury (Fig. 8, Tab. 1, Ref. 30). Text in PDF www.elis.sk.

Effects of alprostadil and iloprost on renal, lung, and skeletal muscle injury following hindlimb ischemia–reperfusion injury in rats

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