The effects of Coriandrum sativum L. as feed additive on mucosal immune parameters, antioxidant defence and, immune‐related genes expression in zebrafish ( Danio rerio )

Abstract

This study investigates the effects of Coriander (C. sativum) on the growth, antioxidant, and immune‐associated genes and serum and mucosal immune parameters in zebrafish (D. rerio). The experimental fish were treated with 0 [control], 5, 10 and 20 g/kg coriander supplemented diets for 8 weeks. The results revealed that coriander remarkably increased mucosal immune parameters (the total Immunoglobulin, protease and lysozyme activity). The highest levels of the measured parameters were noticed in 20 g/kg CP treatment. Also, the zebrafish fed 20 g/kg coriander powder showed significantly higher expression gene for lysozyme and TNF‐alpha. The same results were noticed in case of IL‐1B gene expression. In case of sod gene expression there was no significant difference between treatments. However, regarding cat gene expression, significant difference was noticed between 20 g/kg CP treatment and other groups. In addition, no significant changes were observed between coriander fed zebrafish and control treatments regarding GH gene expression level, although IGF‐I remarkable up‐regulate in 20 g/kg coriander powder treatment compared other groups. In conclusion, it can be proposed that dietary Coriander powder can improve mucosal immune parameters and immune and antioxidant genes expression.

Combined and Singular Effects of Ethanolic Extract of Persian Shallot (Allium hirtifolium Boiss) and Synbiotic Biomin®IMBO on Growth Performance, Serum- and Mucus-Immune Parameters and Antioxidant Defense in Zebrafish (Danio rerio)

“…Different types of proteases are known in fish mucus that play an important role in the innate immune system of fish [78]. Consistent with the results of this study, previous studies have shown that the use of synbiotics in the diet increases serum lysozyme and mucus in fish [72].…”

“…It is well documented that the mucosal immune system of fish could be boosted by dietary administration of prebiotics, probiotics, and medicinal plants [71]. For instance, Safari et al [72] evaluated the effect of Coriandrum sativum L. on mucosal immune parameters, antioxidant defense in zebrafish and found that it can be proposed that dietary coriander powder can improve mucosal immune parameters and immune and antioxidant genes expression. Also, Karimi Pashaki et al [73] found that the dietary garlic extract had a positive effect on survival rate, blood and immune parameters changes, and disease resistance of common carp.…”

Effects of dietary nutmeg (Myristica fragrans) seed meals on growth, non-specific immune indices, antioxidant status, gene expression analysis, and cold stress tolerance in zebrafish (Danio rerio)

“…Plants are being studied as immunomodulators, especially for improving fish performance, immune systems, and disease resistance, thus it has long been recognized that medicinal herbs and spices are among the most important targets ( 34 – 36 ). Medicinal herbs, as eco-friendly substances, provide a variety of benefits, including growth and resistance promoters against environmental shocks, thermal and pollution stress, and parasites ( 1 , 6 , 12 , 27 – 30 ). In this sense, our current study was conducted to evaluate fish growth performance after feeding powdered nutmeg seed, following our previous studies on zebrafish fed with plants, namely ferula ( Ferula assafoetida ) ( 37 ), myrtle ( Myrtus communis ) ( 38 ), and coriander ( Coriandrum sativum ) ( 30 ).…”

“…The sequences of primers used in real-time PCR ( Table 3 ) were selected from our previous studies ( 30 ). Quantitative real-time PCR (qPCR) was performed using an iCycler (Bio-Rad, U.S.A.) with Fermentas Maxima SYBR Green qPCR Master Mix (Fermentas) and gene-specific primers following thermal profile: 94°C during 5 min, 40 cycles at 10 s at 94°C, followed by 10 s at 59°C and 10 s at 72°C and each reaction was amplifying in triplicate ( Table 2 ).…”

Dietary oak ( Quercus brantii ) acorn extract affects growth performance, survival rate, haemolymph parameters, and body composition of narrow‐clawed crayfish ( Pontastacus leptodactylus )

“…Immunostimulants are chemical drugs, stressor or action that improves the innate and adaptive immunity (Keyshams et al, 2021; Song et al, 2014) Herbal extracts are a kind of immunostimulant that have been widely used to enhance the immune system (non‐specific and specific), prevent, and treat several infectious diseases (Vaseeharan & Thaya, 2014). In addition, herbal extracts improve growth performance (Awad & Awaad, 2017; Samadi et al, 2016), induce digestive enzymes (Raissy et al, 2022), and antioxidant defence (Safari et al, 2019).…”