The Effects of hydrocortisone on glycogen storage and phosphorylase activity of frog liver

Hunter, Norvell W.; Johnson, Charles E.

doi:10.1002/jcp.1030550310

Cited by 18 publications

(1 citation statement)

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“…No significant decrease in total hepatic phosphorylase activity after cortisol administration has been reported. In fact, most studies show an increased activity (33,34) occurring as early as 12 hours after steroid administration (32). Although Steiner, Rauda, and Williams found an increase in mean glycogen synthetase activity from 2.4 to 3.7 umoles per g of liver per hour within 5 hours after prednisolone injection in rats, they did not consider this rise significant (35).…”

Section: Discussionmentioning

confidence: 99%

The Acute Effect of Hydrocortisone on Hepatic Glucose Output and Peripheral Glucose Utilization*

Lecocq¹,

Mebane²,

Madison³

1964

J. Clin. Invest.

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There is abundant experimental evidence indicating that adrenal glucocorticoids have a profound effect on carbohydrate metabolism (1-9). Agreement is general that they accelerate hepatic gluconeogenesis and increase liver glycogen stores (5-9), but their action on peripheral glucose utilization is still unsettled (5,6,8). Although the effects of the chronic administration of glucocorticoids are well defined and changes in carbohydrate metabolism have been recorded as early as 2 to 4 hours (10-18) after adrenocorticoid administration, the precise time of onset of glucocorticoid-induced alterations in carbohydrate metabolism has not been established. An immediate action on glucose metabolism has not been demonstrated in vivo; indeed, recent studies have suggested that the effect of adrenocorticoids on carbohydrate metabolism is delayed for at least 2 hours, beginning at a time when steroid concentrations are decreasing (10,13, 14). The changes in glucose metabolism occurring in the earliest period after glucocorticoid administration are especially * Submitted for publication July 12, 1963; accepted October 17, 1963. This work was supported in part by a U. S. Public Health Service grant (A-4236) from the National Institute of Arthritis and Metabolic Diseases, Bethesda, Md., and by grants from the Upjohn Co., Kalamazoo, Mich., and Chas. Pfizer & Co., New York, N. Y.A preliminary report of this work has been published in abstract form (The paradoxical effect of hydrocortisone on hepatic glucose output. Clin. Res. 1961, 9, 29 important to define since obscuring secondary effects are less likely to be present.The purpose of the present study was 1) to delineate the earliest detectable change in carbohydrate metabolism after acute glucocorticoid administration, 2) to characterize the early effects of glucocorticoids on hepatic glucose output, and 3) to determine whether glucocorticoids alter peripheral glucose utilization. Changes in hepatic glucose output and peripheral glucose utilization were followed during the initial 90 to 120 minutes after the intravenous injection of cortisol in dogs with chronic end-to-side portacaval shunts. This preparation was chosen because it separates the liver from the extrahepatic splanchnic circulation and therefore permits measurement of hepatic rather than splanchnic glucose balance. From the changes in hepatic glucose output and arterial glucose concentration, changes in peripheral glucose utilization can be calculated (19). MethodsThirteen studies were done on dogs with complete endto-side portacaval shunts. At least 1 month was allowed for complete recovery from the operative procedure before experiments were performed. Stable weights were maintained in these animals by a diet, supplemented with vitamins, containing 5&% of the total calories as carbohydrate, 20 to 30% as protein, and the remainder as fat.Studies were performed after a 15-hour, overnight fast with Nembutal anesthesia (25 mg per kg). Hepatic venous samples were collected through a cardiac catheter inserted deep...

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Section: Discussionmentioning

confidence: 99%