The Effects of Hydroalcoholic Extract of Apium graveolens Leaf on the Number of Sexual Cells and Testicular Structure in Rat

Abstract

Background:Use of medicinal plants with high antioxidant properties could be effective to increase fertility and improvement of disorders such as hormonal imbalance, impotency, oligospermia and immotile sperm. Celery (Apium graveolens) is rich in antioxidant agents. The leaf and stems of celery contain phenols, furanocoumarin and luteolin. Apigenin is one of the main flavonoids of celery leaf. Objectives: This study aimed to investigate the effects of hydroalcoholic extract of celery on histological properties of testis and number of sexual cells in male rats. Materials and Methods: Thirty-two male Wistar rats were divided into four groups of eight rats each. Control, did not receive any medication; sham, received normal saline; and two groups received celery extract orally in dosages of 100 and 200 mg/kg/BW once every two days for 60 days. At the end, animals were anesthetized, and caudal part of the right epididymis was used for sperm counting. After fixation of testis, tissue sections were prepared and studied microscopically to evaluate morphometric (lumen diameter, number of primary spermatocyte and sertoli cell) and histological changes. Data was analyzed by one-way ANOVA test using SPSS15 software. P < 0.05 was considered as statistically significant. Results: There was a significant increase in the number of sperms, sertoli cells, and primary spermatocyte (P < 0.05) in groups receiving extract; however, structural changes were not observed in the groups. Conclusions: It seems that celery increases spermatogenesis in male rats, but has no destructive effects on testicular tissue.

Ameliorating effects of fennel and cumin extracts on sperm quality and spermatogenic cells apoptosis by inducing weight loss and reducing leptin concentration in diet-induced obese rats

“…It has inhibitory effects on cyclooxygenase and lipoxygenase. Therefore, it is able to reduce detrimental effects of free radicals on cells and prevents cell death due to its antioxidant properties (Kooti et al., ). Fennel contains anethole as the most intense antioxidant (Malo et al., ).…”

Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats

“…The control group received 1 mL of distilled water (as an extraction solvent), and the experimental groups were given 1 mL of hydro-alcoholic extract of fennel seed by oral gavage at respective doses of 35, 70, 140, and 280 mg/kg b.w. daily for 60 days (10,18). The rats were deeply anaesthetised by xylazine (10 mg/kg) and ketamine (60 mg/kg) (Alfasan, the Netherlands) 24 h after the last dose.…”

Effect and Mechanism of Herbal Ingredients in Improving Diabetes Mellitus Complications

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