The effects of hyaluronic acid hydrogels with tunable mechanical properties on neural progenitor cell differentiation

Seidlits, Stephanie K.; Khaing, Zin Z.; Petersen, Rebecca R.; Nickels, Jonathan D.; Vanscoy, Jennifer E.; Shear, Jason B.; Schmidt, Christine E.

doi:10.1016/j.biomaterials.2010.01.125

Cited by 438 publications

(429 citation statements)

References 60 publications

(99 reference statements)

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“…Indeed, in a reaction with 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDCI), the yield of immobilization of furfurylamine on HA was much lower than that using DMTMM (Figure 9 and Figure S3). The structure and degree of substitution were HA-PEG hydrogels were synthesized by mixing solutions of HA-furan an included in HA hydrogels to vary the mechanical properties and pore size of the scaffolds [65][66][67][68] By controlling the chain length of the PEG cross-linker, one can control these properties.…”

Section: Synthesis and Characterization Of Ha-furanmentioning

confidence: 99%

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

2011

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Hyaluronic acid (HA) is a naturally occurring polymer that holds considerable promise for tissue engineering applications. Current cross-linking chemistries often require a coupling agent, catalyst, or photoinitiator, which may be cytotoxic, or involve a multistep synthesis of functionalized-HA, increasing the complexity of the system. With the goal of designing a simpler one-step , aqueous-based cross-linking system, we synthesized HA hydrogels via Diels-Alder "click" chemistry. Furan-modified HA derivates were synthesized and cross-linked via dimaleimide poly(ethylene glycol). By controlling the furan to maleimide molar ratio, both the mechanical and degradation properties of the resulting Diels-Alder cross-linked hydrogels can be tuned. Rheological and degradation studies demonstrate that the Diels-Alder click reaction is a suitable cross-linking method for HA. These HA cross-linked hydrogels were shown to be cytocompatible and may represent a promising material for soft tissue engineering.iii

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Section: Synthesis and Characterization Of Ha-furanmentioning

confidence: 99%

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

2011

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“…As a biomaterial, it is biodegradable, biocompatible and resorbable. It is composed of hydrophilic polymeric chains and has numerous advantages compared with other hydrogels, since it can be easily processed, delivered in a minimally invasive way, and has mechanical properties similar to tissues [36,37]. In addition, its degradation rate and the diffusion of active molecules through it can be modulated by selecting a specific range of molecular weights, or by using different crosslinking agents and fabrication procedures.…”

Section: Introductionmentioning

confidence: 99%

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

et al. 2016

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Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-L-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10 days.The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5 mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system.

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“…The physical and chemical properties of these hydrogels can be tailored to construct a biomaterial for tissue regeneration. Several physical properties have been evaluated in some studies, such as stiffness 26,27 , the size of the pores 28 and the surface charge 22 . In this study, we prepared HEMA-MOETACL hydrogel which has been shown to carry a positive electric charge.…”

Section: Discussionmentioning

confidence: 99%

Repair of spinal cord injury by implantation of bFGF-incorporated HEMA-MOETACL hydrogel in rats

Chen

Yang

et al. 2015

Sci Rep

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There is no effective strategy for the treatment of spinal cord injury (SCI). An appropriate combination of hydrogel materials and neurotrophic factor therapy is currently thought to be a promising approach. In this study, we performed experiments to evaluate the synergic effect of implanting hydroxyl ethyl methacrylate [2-(methacryloyloxy)ethyl] trimethylammonium chloride (HEMA-MOETACL) hydrogel incorporated with basic fibroblast growth factor (bFGF) into the site of surgically induced SCI. Prior to implantation, the combined hydrogel was surrounded by an acellular vascular matrix. Sprague-Dawley rats underwent complete spinal cord transection at the T-9 level, followed by implantation of bFGF/HEMA-MOETACL 5 days after transection surgery. Our results showed that the bFGF/HEMA-MOETACL transplant provided a scaffold for the ingrowth of regenerating tissue eight weeks after implantation. Furthermore, this newly designed implant promoted both nerve tissue regeneration and functional recovery following SCI. These results indicate that HEMA-MOETACL hydrogel is a promising scaffold for intrathecal, localized and sustained delivery of bFGF to the injured spinal cord and provide evidence for the possibility that this approach may have clinical applications in the treatment of SCI.

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The effects of hyaluronic acid hydrogels with tunable mechanical properties on neural progenitor cell differentiation

Cited by 438 publications

References 60 publications

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

Diels−Alder Click Cross-Linked Hyaluronic Acid Hydrogels for Tissue Engineering

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

Repair of spinal cord injury by implantation of bFGF-incorporated HEMA-MOETACL hydrogel in rats

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