The effects of humic water on endothelial cells under hyperglycemic conditions: inflammation-associated parameters

Abstract

Humic waters (HW) are globally unique, deep underground, dark-brown waters containing humic acids, and they present numerous therapeutic activities including anti-inflammatory. In the present study, we use HW from source in Poland. Diabetes has become an epidemic and is a risk factor of cardiovascular diseases. Hyperglycemia in diabetes is responsible for damaging of the endothelium and increases inflammation on the surface of the vascular lining. The inflammatory process in diabetes is associated with the secretion of inflammatory cytokines by endothelial cells, e.g., tumor necrosis factor alpha (TNFα) and interleukin 6 (IL-6), and with the reduction of cell proliferation. In the study, we used cultures of endothelial cells (HUVEC line—human umbilical vein endothelial cells) with the addition 30 mM/L of glucose in the culture medium which imitated the conditions of uncontrolled diabetes. The addition of HW in the proper volume to the culture medium causes reduction of inflammation by significant decrease in inflammatory cytokines such as TNFα and IL-6 and also leads to enhancement of the cell proliferation. It appears that the adverse effects of hyperglycemia on vascular endothelial cells may be corrected by addition of humic water. The above promising results of in vitro tests provide an opportunity to the possible use of humic water in the supportive treatment of endothelial dysfunction disorders in diabetes. However, this issue requires further clinical research.

Antiflammatory activity and potential dermatological applications of characterized humic acids from a lignite and a green compost

“…It has been suggested that the antioxidant and protective role of humic materials may be related to their redox properties 40 , which may inactivate reactive oxygen species by their binding to the humic structure through a reversible and reproducible process 41 . This behaviour may well explain our results indicating that addition of humic matter induces a normal proliferation of HaCaT, cells despite the Urban Dust treatment 42 . However, no univocal structure–activity relationship was yet produced to explain the molecular bases of HA bioactivity against human diseases.…”

Roles of Endovascular Calyx Related Enzymes in Endothelial Dysfunction and Diabetic Vascular Complications

“…High glucose induces inflammatory stimuli (El Masri et al, 2020), leading to increased expression of inflammatory factors IL-6 and TNF-α. The increase in these factors is related to AMPK, NF-LB, and STAT3 (reactive oxygen species) signaling pathways, and TNF-α modifies the related mitochondrial respiratory chain redox response role (Szot et al, 2019). Intracellular heparanases have many important biological functions, including regulating autophagy, and cell communication and survival.…”

“…On the one hand, this causes the adhesion and rolling of macrophages and monocytes; on the other hand, it activates the NF-LB pathway and upregulates the expression of HPSE, MMPs, HYAL, HAS, and NEU (Sieve et al, 2018a). Then, the hyperglycemic inflammatory environment and the degradation of the glycocalyx form a vicious circle (Lemkes et al, 2010;Dogne et al, 2016;Szot et al, 2019). In this cycle, glycocalyx-degradation-related enzymes play important roles.…”

Modification of the chemically induced inflammation assay reveals the Janus face of a phenol rich fulvic acid

“…Both, pro- and anti-inflammatory effects of HSs have been reported. HSs reduced the inflammatory effect of hyperglycemia on vascular endothelial cells, decreased edema, skin diseases, and rheumatoid arthritis 13 – 15 . On the other hand, the generation of excess ROS and oxidative damage has been reported as well, associated for example with the Blackfoot disease 16 – 18 .…”