The effects of homocysteine and nitric oxide inhibitor, applied separately and simultaneously, on acetylcholinesterase activity in the rat heart tissue

Hadžibegović, Adi; Ćuk, Jelena

doi:10.5937/mp67-12602

Cited by 1 publication

(1 citation statement)

References 14 publications

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“…14 The previous study demonstrated that the combination of Hcy and ZnPPR IX has led to increased activity of AChE in relation to the control, suggesting that CO is potentially very important gaseous molecule for mediation of Hcy-induced effects on cardiac AchE. 33 Finally, the limitation of this study could be that determination of certain gasotransmitters effects was not evaluated directly, but indirectly by inhibition of their production. Data on mRNA or protein levels in the cardiac tissue, as well as cellular data would verify the findings that cardiac AChE activity is altered by homocysteine level.…”

Section: Cardiac Tissue Homogenate Ache Activitymentioning

confidence: 92%

The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity

Mutavdzin¹,

Lončar-Stojiljković²,

Kostic³

et al. 2019

Scripta Medica

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Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group-saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip N ω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' sacrifice. AChE activity was measured in the rats' cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+L-NAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.

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Section: Cardiac Tissue Homogenate Ache Activitymentioning

confidence: 92%