The effects of histones in vitro age, and culture state on the digestion of DNA by micrococcal nuclease and deoxyribonuclease I

Dell’Orco, Robert T.; Whittle, W.L.; Duncan, Michael R.; Robinson, Margaret J.

doi:10.1016/0047-6374(83)90046-5

Cited by 9 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These authors attributed the variations in endogenous enzyme activity to differences in acceptor availability. An age-related decline in the accessibility of poly-(ADP-ribose) polymerase to acceptor proteins may parallel other results from both in vivo and in vitro systems in which accessibility of DNA to nucleases is restricted in older populations (Zongza and Mathias, 1979;Tas and Walford, 1982;Dell'Orco et al, 1983). This reported decline in accessibility of nucleases was attributed to changes in chromatin structure in older populations which limit the ability of these enzymes to react with DNA.…”

Section: Methodsmentioning

confidence: 58%

“…Two HDF lines were used in these experiments. CF-3 cells were derived from newborn foreskin and have a characteristic in vitro life span of 65 2 10 population doublings (PD) (Dell'Orco et al, 1983). IMR-91 cells, obtained from the Coriell Institute for Medical Research, Camden, NJ, were derived from fetal lung and have a life span of 50 to 54 PD (Nichols et a]., 1983).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Decline of poly(ADP‐ribosyl)ation during in vitro senescence in human diploid fibroblasts

Dell’Orco

Anderson

1991

Journal Cellular Physiology

View full text Add to dashboard Cite

Poly(ADP-ribose) polymerase activity was determined at various times during the in vitro life span of two human diploid fibroblast-like cell lines of different donor ages. The cell lines differed in their ability to transfer ADP-ribose, with cells from an embryonic donor exhibiting 2 to 3 times the activity found in cells obtained from a newborn donor. The activity in both cell lines decreased by 30-60% as the cells moved through their in vitro life spans. The decline could not be attributed to increases in glycohydrolase or the leakage of polymerase from older cell preparations. Enzyme activation with DNase I indicated that similar levels of enzyme were present in both cell lines at all in vitro ages. These results indicate that although poly(ADP-ribosyl)ation is inversely related to donor age as well as in vitro age the decrease is in response to other factors which change with increasing age.

show abstract

Section: Methodsmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%