The effects of histamine H1 type receptor (H1R) in regulating osteoblastic cell differentiation and mineralization

Sun, Yanpeng; Peng, Xiaodong; Li, Yanzhou; Ma, Husheng; Li, Dongfang; Shi, Xiangqin

doi:10.1080/21691401.2019.1596924

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…Intestinal bacteria decarboxylate the basic amino acid histidine to histamine which plays important roles in immunoregulation [ 68 ]. Histamine activates histamine H1 type receptor (H1R) which suppresses osteoblastogenesis and mineralization [ 69 ]. Histidine metabolism is at least partially implicated in bone formation [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Qiao,

Li,

Wang

et al. 2024

Aging

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Background: The gut microbiota (GM) constitutes a critical factor in the maintenance of physiological homeostasis. Numerous studies have empirically demonstrated that the GM is closely associated with the onset and progression of osteoporosis (OP). Nevertheless, the characteristics of the GM and its metabolites related to different forms of OP are poorly understood. In the present study, we examined the changes in the GM and its metabolites associated with various types of OP as well as the correlations among them. Methods: We simultaneously established rat postmenopausal, disuse-induced, and glucocorticoid-induced OP models. We used micro-CT and histological analyses to observe bone microstructure, three-point bending tests to measure bone strength, and enzyme-linked immunosorbent assay (ELISA) to evaluate the biochemical markers of bone turnover in the three rat OP models and the control. We applied 16s rDNA to analyze GM abundance and employed untargeted metabolomics to identify fecal metabolites in all four treatment groups. We implemented multi-omics methods to explore the relationships among OP, the GM, and its metabolites. Results: The 16S rDNA sequencing revealed that both the abundance and alterations of the GM significantly differed among the OP groups. In the postmenopausal OP model, the bacterial genera g__Bacteroidetes_unclassified, g__Firmicutes_unclassified, and g__Eggerthella had changed. In the disuse-induced and glucocorticoid-induced OP models, g__Akkermansia and g__Rothia changed, respectively. Untargeted metabolomics disclosed that the GM-derived metabolites significantly differed among the OP types. However, a Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that it was mainly metabolites implicated in lipid and amino acid metabolism that were altered in all cases. An association analysis indicated that the histidine metabolism intermediate 4-(β-acetylaminoethyl) imidazole was common to all OP forms and was strongly correlated with all bone metabolism-related bacterial genera. Hence, 4-(β-acetylaminoethyl) imidazole might play a vital role in OP onset and progression. Conclusions: The present work revealed the alterations in the GM and its metabolites that are associated with OP. It also disclosed the changes in the GM that are characteristic of each type of OP. Future research should endeavor to determine the causal and regulatory effects of the GM and the metabolites typical of each form of OP.

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Section: Discussionmentioning

confidence: 99%

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Qiao,

Li,

Wang

et al. 2024

Aging

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“…239 Sun et al found that histamine could activate the histamine H1 type receptor (H1R) to suppress osteoblastic differentiation and mineralization in MC3T3-E1 cells. 240 It is clear that gut microbial metabolites of histamine are involved in bone formation, at least in part. In an interesting study, exposure to a warm temperature (34℃) was shown to protect against bone loss through the GM, while polyamines (spermine and spermidine), the microbial metabolites of Orn, were reported to be the key mediators of this process.…”

Section: Basic Amino Acidsmentioning

confidence: 99%

“…Histidine, a basic amino acid, can be decarboxylated to histamine by gut bacteria and plays an important role in immune regulation 239 . Sun et al found that histamine could activate the histamine H1 type receptor (H1R) to suppress osteoblastic differentiation and mineralization in MC3T3‐E1 cells 240 . It is clear that gut microbial metabolites of histamine are involved in bone formation, at least in part.…”

Section: Gm‐derived Metabolites and Bone Metabolismmentioning

confidence: 99%

Gut microbiota and bone metabolism

Chen

Liu

et al. 2021

The FASEB Journal

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Osteoporosis is the most common metabolic skeletal disease. It is characterized by the deterioration of the skeletal microarchitecture and bone loss, leading to ostealgia, and even bone fractures. Accumulating evidence has indicated that there is an inextricable relationship between the gut microbiota (GM) and bone homeostasis involving host–microbiota crosstalk. Any perturbation of the GM can play an initiating and reinforcing role in disrupting the bone remodeling balance during the development of osteoporosis. Although the GM is known to influence bone metabolism, the mechanisms associated with these effects remain unclear. Herein, we review the current knowledge of how the GM affects bone metabolism in health and disease, summarize the correlation between pathogen‐associated molecular patterns of GM structural components and bone metabolism, and discuss the potential mechanisms underlying how GM metabolites regulate bone turnover. Deciphering the complicated relationship between the GM and bone health will provide new insights into the prevention and treatment of osteoporosis.

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“…Osteoporosis is attributable to an imbalance in bone homeostasis that is mainly regulated by osteoblasts and osteoclasts (Reginster & Burlet, 2006). Differentiation and mineralization in osteoblasts play a crucial part in bone formation, while differentiation in osteoclasts is involved in bone resorption (Boyle, Simonet, & Lacey, 2003; Sun et al, 2019). Osteoporosis is considered to be a severe health issue and 160 million people are suffering from this disease in China (Li, Li, Chen, & Xu, 2020).…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg3 attenuates ovariectomy‐induced osteoporosis via AMPK/mTOR signaling pathway

Zhang

Huang

Chen

et al. 2020

Drug Development Research

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Ginsenoside Rg3, a ginsenoside isolated from Panax ginseng, can regulate autophagy via AMP‐activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signaling pathway. AMPK/mTOR signaling and autophagy have been reported to be involved in osteogenesis. Here, the effect of Rg3 on ovariectomy (OVX)‐induced osteoporosis is explored. In vivo, rats were treated with 20 mg/kg Rg3 after OVX and the body weight (BW) was monitored. Bone mineral density (BMD), hematoxylin–eosin staining of femur tissues, osteogenesis, autophagy, and AMPK/mTOR signaling were analyzed. In vitro, MC3T3‐E1 cells were treated with 0, 1, 5, 10, 20, and 100 μmol/L Rg3. 10 and 20 μmol/L Rg3, which had no significant effect on cell viability and significantly affected AMPK/mTOR signaling, were chosen for further analysis. Then osteogenic differentiation was induced with Rg3 or/and AMPK inhibitor (Compound C). AMPK/mTOR signaling, autophagy, osteogenic differentiation, and mineralization by Alizarin Red staining were analyzed. The expression or activity of AMPK/mTOR signaling‐related proteins, autophagy markers, and osteogenesis markers was measured by western blotting or commercial kits, and cell viability by cell counting kit‐8 assay kits. Rg3 significantly alleviated OVX‐induced BW increases, BMD declines and histological changes of femur tissues, promoted osteogenesis, autophagy, and AMPK signaling, but inhibited mTOR signaling in vivo. Moreover, Rg3 significantly enhanced AMPK signaling, autophagy, osteogenic differentiation, and mineralization, but suppressed mTOR signaling in vitro. However, Compound C significantly reversed Rg3‐induced alterations in vitro, indicating that Rg3 regulated autophagy, osteogenic differentiation, and mineralization via AMPK/mTOR signaling. Hence, it was speculated that Rg3 might attenuate OVX‐induced osteoporosis via AMPK/mTOR signaling pathway.

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The effects of histamine H1 type receptor (H1R) in regulating osteoblastic cell differentiation and mineralization

Cited by 9 publications

References 33 publications

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Gut microbiota and bone metabolism

Ginsenoside Rg3 attenuates ovariectomy‐induced osteoporosis via AMPK/mTOR signaling pathway

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