The Effects of Gonadotropin Immunization of Prepubertal Rabbits on Gonadal Development

R, Monastirsky; Laurence, Kenneth A.; Tovar, Enrique

doi:10.1016/s0015-0282(16)38228-0

Cited by 13 publications

(4 citation statements)

References 9 publications

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“…Monastirsky et al 1971;Turner & Johnson 1971). However, these results must be interpreted with caution as the antibodies used were not specific for FSH.…”

mentioning

confidence: 99%

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

Wickings

Usadel

Dathe

et al. 1980

Acta Endocrinologica

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In the rat, whilst FSH has been shown to be necessary for the initiation of spermatogenesis, this hormone does not appear to be involved in the maintenance of spermatogenesis in the mature animals. Since the role of FSH has not been conclusively established in primate species, including humans, a study based on the neutralization of FSH by passive immunization with specific FSH antibodies was initiated in adult male rhesus monkeys.Prior to the actual study an antiserum pool was generated from 4 monkeys actively immunized with highly purified ovine FSH. In this FSH antiserum there was no evidence for the existence of LH antibodies, and the capacity of the antiserum to neutralize rhesus FSH was demonstrated by bioassay. Because of marked circannual variations in testicular functions passive immunization with FSH-antiserum was performed during the period of full testicular development in 4 other monkeys. FSH antiserum was injected for 106 days (i.e. a period covering the full duration of the total spermatogenetic process twice). Four further monkeys serving as controls were injected with normal rhesus serum for the same period. Testicular volume and histology, anti-FSH-titer, seminal parameters, serum testosterone, and LH were monitored at regular intervals. In the passively immunized animals the testicular volume decreased by 50%, sperm counts decreased below control counts, histology revealed reduction of the tubular diameter by 40%, derangement of spermatogenesis and, on instances, tubuli with Sertoli cells only. Leydig cells appeared histologically normal, although testosterone levels in the passively immunized monkeys tended to be lower than in control animals. Values were not significantly different at the end of the observation period.From these results it can be concluded that in contrast to the rat FSH plays a specific role in the maintenance of spermatogenesis in the mature rhesus monkey.

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“…Monastirsky et al 1971;Turner & Johnson 1971). However, these results must be interpreted with caution as the antibodies used were not specific for FSH.…”

mentioning

confidence: 99%

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

Wickings

Usadel

Dathe

et al. 1980

Acta Endocrinologica

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“…Sairam, unpublished results). This was most surprising, contrary to well-documented gonadal regression in all animals caused by active immunization using native gonadotropins (Monastirsky et al 1971). Subsequently, it was found that deglycosylation altered antigenicity of the molecule (Rebois and Liss 1987;Sairam et al 1988).…”

Section: Discussionmentioning

confidence: 60%

Influence of carbohydrates on the antigenic structure of gonadotropins: distinction of agonists and antagonists

Sairam¹,

Linggen²,

Sairam³

et al. 1990

Biochem. Cell Biol.

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The biological properties of glycosylated (native) and deglycosylated gonadotropins are different. The immunological characteristics of antibodies prepared against deglycosylated lutropin and human chorionic gonadotropin were investigated. Distinct antibodies of rabbit polyclonal antisera against deglycosylated lutropin and deglycosylated chorionic gonadotropin were separated by affinity chromatography on divinylsulfonyl-Sepharose-immobilized hormone or antagonist columns, respectively, in successive runs. Antibodies that could discriminate between agonist and antagonistic forms of the hormones could thus be obtained. In radioimmunoassays using 125I-labeled antagonists and respective antagonist antibodies, only the deglycosylated hormones or their deglycosylated alpha-subunits showed preferential reaction. Based on recombinations using different deglycosylated subunits, it was concluded that the loss of antennary sugars in the alpha-subunits was mainly responsible for the changes that led to the formation of antagonist-specific antibodies. Only the agonist-specific antibody could neutralize hormone action. Thus, the type and extent of glycosylation appears to influence the antigenic structure of these secreted glycoproteins.

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“…Neutralization of endogenous pituitary gonadotrophins by antibodies has been demonstrated previously by several authors (Segal et al, 1962;Talaat & Laurence, 1969;Schwartz & Ely, 1970;Monastirsky et al, 1971;Schuchner & Monastirsky, 1973). Talaat & Laurence (1969) showed that active FSH immunization in rabbits diminished their fertility.…”

mentioning

confidence: 66%

“…Recently, it has been demonstrated that foreign gonadotrophins are antigenic in the rabbit (Segal, Laurence, Perlbachs & Hakim, 1962;Talaat & Laurence, 1969;Schwartz & Ely, 1970;Monastirsky, Laurence & Tovar, 1971;Schuchner & Monastirsky, 1973). The aim of this study was to describe the effects of active anti-FSH immunization on the ovarian morphology of adult rabbits and the possible mechanism of action of FSH.…”

mentioning

confidence: 99%

Immunization of Rabbits Against FSH and Effects on the Zona Pellucida

R¹,

Schuchner²,

Deu³

1974

Reproduction

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The effects of active FSH immunization on the ovarian morphology of adult rabbits were studied. Rabbits were immunized intradermally in multiple sites with antibodies to ovine FSH. Both the macroscopic morphology and the weight of the ovaries of the experimental rabbits were similar to those of the controls. Multiple branched projections of the granulosa cells were seen in the experimental rabbits. These projections had higher electron density than the rest of the cell. The zona pellucida was not formed although the growing follicles were large enough to have a completely formed layer. Disturbance of the metabolism of the follicular cells, due to the lack of endogenous FSH, could explain this abnormality.

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The Effects of Gonadotropin Immunization of Prepubertal Rabbits on Gonadal Development

Cited by 13 publications

References 9 publications

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

The role of follicle stimulating hormone in testicular function of the mature rhesus monkey

Influence of carbohydrates on the antigenic structure of gonadotropins: distinction of agonists and antagonists

Immunization of Rabbits Against FSH and Effects on the Zona Pellucida

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