The effects of glyphosate exposure on gene transcription and immune function of the silkworm, <i>Bombyx mori</i>

Feng, Piao; Wang, Yuanfei; Zhang, Hongbin; Zhu, Qingyu; Ren, Yuying; Shu, Qilong; Su, Wujie; Liu, Wei; Hu, Yufang; Li, Bing

doi:10.1002/arch.21990

Cited by 6 publications

(2 citation statements)

References 57 publications

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“…cDNA was synthesized using oligo‐dT and random primer. Detailed methodology for qRT‐PCR was previously described (Feng et al., 2023). The relative transcript levels of target genes were calculated using the 2 ‐ΔΔCt method, and RP49 was used as the reference gene.…”

Section: Methodsmentioning

confidence: 99%

Effects of different diets on thermal tolerance in the silkworm, Bombyx mori

Wang,

Zhang,

Tian

et al. 2023

Entomologia Exp Applicata

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The survival of species is negatively impacted by the increased frequency and intensity of high‐temperature episodes caused by increased global warming. Thermal tolerance can influence population size and the geographic distribution of species, which translates into species‐wide responses to climate and affects interactions between communities of species. The silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), is an ectotherm and an internationally recognized model insect for studying sensitivity to the environment. In the present study, we investigated the effects of different diets on the thermal tolerance of silkworm. As such, it was identified that silkworms reared on an artificial diet in instars I–III and mulberry leaves in instar IV (referred as the AM group) exhibited faster weight gain and higher antioxidant enzyme activity at 35 °C, whereas silkworms reared only on mulberry leaves (referred as the ML group) exhibited upregulated transcript levels of key genes regulating antioxidant enzyme signaling pathways including Akt, CncC, and keap1, significantly increased reactive oxygen species (ROS) levels, and severely impaired midgut activity at 35 °C. Thus, the AM group presented stronger thermal tolerance. These findings suggest that diet can affect the thermal tolerance of silkworm. This research improved our understanding of the molecular, biochemical, and physiological changes caused by thermal stress and laid the groundwork for future research regarding the mechanism underlying thermal tolerance. Furthermore, this study provides an important reference for the conservation of biodiversity in the context of global warming.

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Section: Methodsmentioning

confidence: 99%

Effects of different diets on thermal tolerance in the silkworm, Bombyx mori

Wang,

Zhang,

Tian

et al. 2023

Entomologia Exp Applicata

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“…For example, direct exposure to glyphosate decreases locomotor ability in termites (Ekaye et al 2022) and cockroaches (Kanabar et al 2021), as well as reduces the level of y response and learning capacities in mosquito larvae (Baglan et al 2018). Direct exposure to glyphosate may also alter physiological traits including immunocompetence and immunity, as reported in silkworms (Feng et al 2023) and bees (Grassl et al 2018). Broader effects of direct and indirect exposure to glyphosate have also been reported at the level of whole arthropod communities, for example in crops, where the presence of glyphosate in the environment reduces the density of predators such as ants, spiders and beetles due to altered foraging behaviour (Pereira et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Limited effects of glyphosate on the behaviour and immunity of males from six populations of the European earwig

Pasquier,

Lécureuil,

Meunier

2024

Preprint

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The use of herbicides on crops often results in unintentional, low-dose exposure of non-target organisms, such as insects. While these exposures are increasingly known to alter the survival and physiology of insects, it remains unclear whether these effects can vary between populations and modify other fitness-related traits, such as behaviour and immunity. Here, we addressed these questions by testing the effects of sublethal exposure to glyphosate (Roundup©) on the behaviour and immunity of European earwig males from six natural populations. We exposed each male to a dose of glyphosate that was either recommended for crops, five times lower than that recommended for crops, or to a control solution. Twenty-four hours later, we measured the activity, boldness, and aggregation of each male. We then exposed them to an entomopathogenic fungus, monitored their survival for 6 weeks, and measured the immune response of the survivors. We found a condition-dependent effect of glyphosate exposure on male activity. Exposure to low doses induced a positive association between activity and weight, which was not observed in the high-dose and control groups. Glyphosate had no effect on any of the other measured traits. All these results were consistent across the six populations tested, although we did find population-specific differences in almost all measurements on males. Further research is now needed to better comprehend the non-linear dose-response to glyphosate on male activity and to evaluate the effectiveness of detoxification processes in this species. Overall, these results emphasise the importance of investigating the effects of herbicides on insects to expand our general understanding of the use and potential risks of plant protection products in integrated pest management programs.

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Limited effects of a glyphosate-based herbicide on the behaviour and immunity of males from six populations of the European earwig

Pasquier,

Lécureuil,

Meunier

2024

Environ Sci Pollut Res

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The effects of glyphosate exposure on gene transcription and immune function of the silkworm, Bombyx mori

Abstract: Glyphosate is a widely used herbicide and crop desiccant.However, whether its extensive use has any effect on the species diversity of nontarget organisms is still unclear. In this study, we used the silkworm, Bombyx mori, as the

Cited by 6 publications

References 57 publications

Effects of different diets on thermal tolerance in the silkworm, Bombyx mori

Effects of different diets on thermal tolerance in the silkworm, Bombyx mori

Limited effects of glyphosate on the behaviour and immunity of males from six populations of the European earwig

Limited effects of a glyphosate-based herbicide on the behaviour and immunity of males from six populations of the European earwig

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