The effects of genotypes and irradiation doses on haploid embryo induction and plant production in bottle gourd [<i>Lagenaria siceraria</i>(Malign) Stanley]

Güler, Pakize Gök; Sarı, Nebahat; Yetışır, Halit; Yegül, Mahmut; Kantoglu, Y.; Kunter, Birhan

doi:10.17660/actahortic.2017.1151.22

Cited by 6 publications

(6 citation statements)

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“…Successful parthenogenetic haploid induction in squash (180 um diameter) with lower doses (25 to 50 Gy) gave support for this explanation (Kurtar et al 2002). However, a contrary result was found in Lagenaria genus (60 to 80 um diameter), successful haploids were induced with low doses of 50 to 75 Gy (Teppner 2004;Guler et al 2017). The present work con rmed those ndings in Lagenaria genus, parthenogenetic haploid plants with eleven chromosomes was obtained at lower irradiation doses 100 to 125 Gy.…”

Section: Discussionmentioning

confidence: 90%

“…For the maximum production of haploid plantlets from embryos pollinated with irradiated pollens, days after fecundation when the fertilized embryos were processed is essential. Previous report in bottle gourd (Guler et al 2017) used fruits at 17 to 21 days after pollination for embryo detection, therefore, fruits at the same growth period were prior to detected. A parallel experiment was conducted to detect those fruits harvested before 17 or later than 21 days after pollination, while either no embryoids or haploid embryos were obtained.…”

Section: Discussionmentioning

confidence: 99%

“…It has been documented that bottle gourd rootstocks enhanced disease resistance to Fusarium wilt (Zhang et al 2021) and powdery mildew (Kousik et al 2018); mitigated abiotic stresses, such as heavy metal, drought and salinity (Nawaz et al 2018; Niu et al 2018); and improved the nutrient uptake, plant growth and yield (Zhong et al 2019). To develop resistant bottle gourd varieties, it is necessary to develop inbred lines which usually takes about 8 to 10 years by conventional breeding programme to overcome the problem of heterozygosity (Guler et al 2017). However, producing haploid plants is of outstanding advancement which could generate pure lines in a single generation and thus improve the selection e ciency.…”

Section: Introductionmentioning

confidence: 99%

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Haploid Induction and Plant Production in Bottle Gourd by Pollination with Gamma Irradiated Pollen

Zhang

Zhao

et al. 2022

SSRN Journal

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Haploid Induction and Plant Production in Bottle Gourd by Pollination with Gamma Irradiated Pollen

Zhang

Zhao

et al. 2022

SSRN Journal

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“…Effective irradiation doses for inducing haploid embryos ranged from 50-75 Gy, emphasizing the importance of genotype and irradiation dosage optimization for successful haploid embryo induction. This study was also pursued with E20A liquid media [64]. Kurtar et al examined dihaploidization in generating F1 hybrid summer squash varieties, highlighting the cultivation of embryos on modified E20A medium.…”

Section: Haploidizationmentioning

confidence: 99%

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Reddy,

Ramireddy,

Reddy

2024

Plants

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Embryo rescue is a vital technique in cucurbit breeding and propagation, addressing challenges such as embryo abortion, poor seed viability, and incompatibility barriers. This method involves the excision of immature embryos from seeds followed by their in vitro culture on a nutrient medium, providing an environment conducive to their growth and development. In cucurbits, embryo rescue has been extensively utilized to overcome barriers to hybridization, enabling the production of interspecific and intergeneric hybrids with desired traits. Various factors, including genotype, developmental stage of embryos, and culture conditions, influence the success of embryo rescue in cucurbits. Optimal nutrient formulations, growth regulators, and culture techniques are critical for promoting embryo germination, shoot elongation, and subsequent plantlet establishment. Additionally, embryo rescue facilitates the recovery of valuable genetic material from wild and exotic cucurbit species, expanding genetic diversity and developing novel cultivars with improved traits such as disease resistance, yield, and quality. This review highlights the principles, applications, and advancements in embryo rescue technology in cucurbits, emphasizing its significance in cucurbit breeding programs and crop improvement efforts.

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“…The development of plant biotechnologies opened new opportunities in this respect. One of the methods for accelerated development of haploid and double haploid lines is the gamma-induced parthenogenesis (Todorova et al, 1997;Gok Guler et al, 2015;Blasco et al, 2016;Wang et al, 2016). The use of this method significantly shortens the time necessary to develop parental lines for heterosis breeding in sunflower Ivanov, 1999, 2000).…”

Section: Introductionmentioning

confidence: 99%

Parthenogenetic responsiveness of sunflower hybrid combinations with expressed tolerance to herbicides

Drumeva¹,

Yankov²

2017

AST

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Abstract. For accelerated development of fertility restorer lines with imidazolinone tolerance in sunflower (Helianthus annuus L.), the gamma-induced parthenogenesis, which combines classical breeding approaches and in vitro techniques, was applied. As initial materials six imidazolinone-tolerant hybrid combinations (F1203, F1216, F1246, F1248, F1295 and F1332)Keywords: sunflower, double haploid fertility restorer lines, embryo culture, gamma-induced parthenogenesis AGRICULTURAL SCIENCE AND TECHNOLOGY, VOL. 9, No 3, pp -3, 2017 190 19 DOI: 10.15547/ast.2017 IntroductionThe herbicide-tolerant sunflower hybrids are generally preferred by agricultural producers in Bulgaria and this determines the main directions of contemporary sunflower breeding. Imidazolinone (IMI) and sulfonylurea (SU) tolerance to herbicides transferred to cultural sunflower from wild species is most often used (Kaya et al., 2012).The development of parental components for the hybrid is a necessary prerequisite for the production of sunflower hybrids tolerant to herbicides. The classical breeding approach for developing lines that can be included as parental components of hybrids comprises inbreeding of plants for at least 5 -6 years. To accelerate this process, a number of researchers have directed their attention towards non-traditional approaches for production of homozygotic lines. The development of plant biotechnologies opened new opportunities in this respect. One of the methods for accelerated development of haploid and double haploid lines is the gamma-induced parthenogenesis (Todorova et al., 1997;Gok Guler et al., 2015;Blasco et al., 2016;Wang et al., 2016). The use of this method significantly shortens the time necessary to develop parental lines for heterosis breeding in sunflower Ivanov, 1999, 2000). The properties of the double haploid lines obtained through this method are determined by the genetic potential of the initial material they were derived from. In spite of the unquestionable benefits from the use of biotechnology methods for acceleration of the breeding process, there are many cases in which they are influenced by the genotypic responsiveness of the investigated plant material (Sauton, 1989;Bouvier et al., 1993;Faris et al., 1999;Todorova et al., 2004;Taşkın et al., 2013;Drumeva and Yankov, 2015). Therefore, additional investigations and certain modifications of the standard methodologies are necessary depending on the specific requirements of the genotype.This study was aimed at investigating the genotypic responsiveness of sunflower hybrid combinations with expressed herbicide tolerance to the application of the gamma-induced parthenogenesis method for accelerated production of imidazolinones-tolerant fertility restorer lines. Material and methodsAs initial materials for parthenogenetic induction six imidazolinone-tolerant hybrid combinations (F1203, F1216, F1246, F1248, F1295 and F1332), obtained from Dobrudzha Agricultural Institute, General Toshevo, were used. For initiation of parthenogenetic embryo devel...

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The effects of genotypes and irradiation doses on haploid embryo induction and plant production in bottle gourd [Lagenaria siceraria(Malign) Stanley]

Cited by 6 publications

References 12 publications

Haploid Induction and Plant Production in Bottle Gourd by Pollination with Gamma Irradiated Pollen

Haploid Induction and Plant Production in Bottle Gourd by Pollination with Gamma Irradiated Pollen

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Parthenogenetic responsiveness of sunflower hybrid combinations with expressed tolerance to herbicides

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