The effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species

Głowacka, Katarzyna; Jeżowski, S.; Kaczmarek, Z.

doi:10.1007/s11240-010-9708-6

Cited by 62 publications

(61 citation statements)

References 24 publications

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“…However, the mechanism of totipotency is still less understood. Many factors affect shoot regeneration in plant tissue culture: such as genotype Glowacha et al 2010;Park et al 2011), exogenous and endogenous hormones (Jiménez 2005;Barreto et al 2010;Sun and Hong 2010;Huang et al 2012), carbon sources (Huang and Liu 1998;Iraqi et al 2005;Huang et al 2006;Silva 2010;Feng et al 2010), and osmotic requirements (Geng et al 2008;Pan et al 2010;Huang et al 2012). Despite many shoot regeneration and transformation protocols developed in rice culture, the regeneration frequency is low and varies highly among cultivars (Al-Khayri et al 1996;Hoque and Mansfied 2004;Khaleda and Al-Forkan 2006;Zhao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Lee

Huang

2013

Plant Growth Regul

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This study aimed to clarify the possible mechanism of endogenous phytohormone signaling and carbohydrate metabolism during shoot organogenesis induced by osmotic stress in rice (Oryza sativa L. cv. Tainung 71) callus. Non-regenerable calli derived from Tainung 71 immature embryos were inoculated on Murashige and Skoog medium containing 10 lM 2,4-D. They turned to highly regenerable calli (HRC) (regeneration frequency more than 75 %) with lower calli fresh weight and water content when 0.6 M sorbitol was supplemented into the medium. The regeneration frequency was prominently decreased to 25 % while an auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), was added into the sorbitoltreated medium. It suggested that endogenous auxin signal may be involved in the induction of HRC under osmotic stress treatment. As well, HRC showed high levels of glucose, sucrose, and starch and high expression of cell wall-bound invertase 1, sucrose transporter 1 (OsSUT1), OsSUT2, PIN-formed 1, and late embryogenesis abundant 1 (OsLEA1) genes. Their expressions are all dramatic inhibited except OsLEA1 under TIBA treatment. It suggests a key role of auxin may be linked to the effect of shoot regeneration under osmotic stress treatment. Therefore, we present a putative hypothesis for regenerable calli induction by osmotic stress treatment in rice. Osmotic stress may regulate endogenous levels of auxin interacting with abscisic acid, then affect carbohydrate metabolism to trigger callus initiation and further shoot regeneration in rice.

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Section: Introductionmentioning

confidence: 99%

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Lee

Huang

2013

Plant Growth Regul

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“…Somaclonal variation induced in regenerated M. ×giganteus plants by culture conditions could represent new genotypes (Seok Kim et al, 2010). Miscanthus ×giganteus plant fertility is increased by doubling the chromosome number in vitro and ex vitro to obtain stable hexaploid plants (Głowacka et al, 2009(Głowacka et al, , 2010Yeon Yu et al, 2009). The obtained derivatives have higher pollen viability and produce more seeds than the parents (Głowacka et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Sterility of Miscanthus × Giganteus Results from Hybrid Incompatibility

Słomka¹,

Kuta²,

Płażek³

et al. 2012

Acta Biologica Cracoviensia Series Botanica

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Miscanthus ×giganteus Greef et Deu. (Poaceae), a hybrid of Miscanthus sinensis and M. sacchariflorus native to Japan, is an ornamental and a highly lignocellulosic bioenergy crop, cultivated in the European Union as an alternative source of energy. This grass reproduces exclusively vegetatively, by rhizomes or via expensive in vitro micropropagation. The present study was aimed at finding the barriers that prevent sexual seed production, based on detailed embryological analyses of the whole generative cycle, including microsporogenesis, pollen viability, megasporogenesis, female gametophyte development, and embryo and endosperm formation. Sterility of M. ×giganteus results from abnormal development of both male and female gametophytes. Disturbed microsporogenesis (laggard chromosomes, univalents, micronuclei) was further highlighted by low pollen staining. The frequency of stainable pollen ranged from 13.9% to 55.3% depending on the pollen staining test, and no pollen germination was observed either in vitro or in planta. The wide range of pollen sizes (25.5-47.6 μm) clearly indicated unbalanced pollen grain cytology, which evidently affected pollen germination. Only 9.7% of the ovules developed normally. No zygotes nor embryos were found in any analyzed ovules. Sexual reproduction of M. ×giganteus is severely hampered by its allotriploid (2n=3x=57) nature. Hybrid sterility, a strong postzygotic barrier, prevents sexual reproduction and, therefore, seed formation in this taxon.K Ke ey y w wo or rd ds s: : Miscanthus ×giganteus, allotriploid, pollen viability, female gametophyte development, sterility.

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“…Specifically, 12.5 to 50 mM proline in callus induction media with Murashige & Skoog (MS) salts (Murashige & Skoog, 1962) increased embryogenic callus formation more than media with N6 salts (Chu et al, 1975). The inhibitory effects of proline on tissue browning in Miscanthus were confirmed by another group (Głowacka et al, 2010). Conversely, adding honey instead of sucrose to callus induction media inhibited browning of cultured immature inflorescences, and a combination of the honey and banana pulp was best for inducing regenerable callus in M. × giganteus (Płażek & Dubert, 2009, 2010 (Głowacka et al, 2010).…”

Section: Miscanthus Regeneration Systemsmentioning

confidence: 89%

“…The inhibitory effects of proline on tissue browning in Miscanthus were confirmed by another group (Głowacka et al, 2010). Conversely, adding honey instead of sucrose to callus induction media inhibited browning of cultured immature inflorescences, and a combination of the honey and banana pulp was best for inducing regenerable callus in M. × giganteus (Płażek & Dubert, 2009, 2010 (Głowacka et al, 2010). Mature seeds can also serve as explants for tissue culture in M. sinensis.…”

Section: Miscanthus Regeneration Systemsmentioning

confidence: 91%