The effects of free radical scavengers on the oxidation of low-density lipoproteins by macrophages

Wilkins, Gary M.; Leake, David S.

doi:10.1016/0005-2760(94)90050-7

Cited by 10 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This later effect can result in PON autoinactivation (M. Aviram, unpublished observation). H 2 O 2 is a major ROS produced by arterial wall cells during atherogenesis, and it is converted under oxidative stress into a more potent ROS leading to LDL oxidation (61,62). The ability of HDL-associated PON to hydrolyze H 2 O 2 (in addition to peroxides) may thus play an important role in eliminating potent oxidants that are involved in atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions. A possible peroxidative role for paraoxonase.

Aviram

Rosenblat²,

Bisgaier³

et al. 1998

J. Clin. Invest.

1,047

763

View full text Add to dashboard Cite

HDL levels are inversely related to the risk of developing atherosclerosis. In serum, paraoxonase (PON) is associated with HDL, and was shown to inhibit LDL oxidation. Whether PON also protects HDL from oxidation is unknown, and was determined in the present study. In humans, we found serum HDL PON activity and HDL susceptibility to oxidation to be inversely correlated ( r 2 ϭ 0.77, n ϭ 15). Supplementing human HDL with purified PON inhibited copper-induced HDL oxidation in a concentrationdependent manner. Adding PON to HDL prolonged the oxidation lag phase and reduced HDL peroxide and aldehyde formation by up to 95%. This inhibitory effect was most pronounced when PON was added before oxidation initiation. When purified PON was added to whole serum, essentially all of it became HDL-associated. The PON-enriched HDL was more resistant to copper ion-induced oxidation than was control HDL.

show abstract

Section: Discussionmentioning

confidence: 99%

Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions. A possible peroxidative role for paraoxonase.

Aviram

Rosenblat²,

Bisgaier³

et al. 1998

J. Clin. Invest.

1,047

763

View full text Add to dashboard Cite

show abstract

“…Oxidative stress, caused by cells or free radicals, modifies the lipid and protein components of circulating LDL and alters its properties [5][6][7]. The effects exerted by LDL on human endothelial cells were demonstrated to depend qualitatively and quantitatively on the extent of its oxidation [8][9][10][11]. In contrast to native-LDL, oxidised-LDL induces a cytotoxic effect on endothelial cells and a chemotactic stimulation of smooth muscle cells [12,13].…”

Section: Introductionmentioning

confidence: 99%

The evaluation of the oxidative state of native-LDL: three methods compared

Carru

Zinellu

Galistu

et al. 2004

Journal of Biochemical and Biophysical Methods

View full text Add to dashboard Cite

“…It is also possible that the in vitro oxidation induced by Cu could be particularly drastic and that LDL oxidation induced by other more physiological or mild inducers, such as Table 6. LDL susceptibility to in vitro copper-induced oxidation at the beginning of the study (T0) and after 6-month zinc supplementation (T6)* haem-iron (Grinshtein et al 2003;Klouche et al 2004) or monocytes -macrophages (Wilkins & Leake, 1994;Chisolm et al 1999), may be more appropriate. Consequently, new approaches need to be developed to determine LDL oxidizability with mild inducers, and preferably in their biological medium without isolation and where LDL oxidizability can be measured without elimination of the many protective molecules that LDL may contain when they are in their biological medium.…”

Section: Discussionmentioning

confidence: 99%

Effect of zinc supplementation on in vitro copper-induced oxidation of low-density lipoproteins in healthy French subjects aged 55–70 years:the Zenith Study

et al. 2006

View full text Add to dashboard Cite

Zn has been shown to possess antioxidant properties in vitro and in vivo. As inadequate dietary Zn intake has been reported in these populations, Zn supplementation may protect against oxidative stress and thereby limit the progression of degenerative diseases in such populations. We conducted the present study to evaluate the long-term supplementation effects of two moderate doses of Zn on in vitro Cu-induced LDL oxidation in French men and women. Three groups of sixteen healthy subjects aged 55-70 years from each sex participated in this randomized double-blind, placebocontrolled study. Each group received for six months either 0, 15 or 30 mg supplemental Zn per d. At the beginning and at the end of the supplementation periods, dietary intakes of Zn, Cu, Fe and vitamin E were estimated using 4 d food-intake records (including the weekend) and the GENI program. Zn, Cu, Fe and vitamin E status were also determined. In vitro LDL oxidizability (basal conjugated diene level, maximal conjugated diene formation and lag time) and lipid parameters were also determined. Dietary intakes of Zn, Cu, Fe and vitamin E were adequate in this population. Zn supplementation significantly increased serum Zn levels but did not significantly modify Cu, Fe or vitamin E status. However, Zn supplementation had no effect on in vitro LDL oxidation parameters, nor were there any sex-related differences in in vitro LDL oxidizability. The present study showed that long-term Zn supplementation of healthy subjects aged 55 -70 years had no effect on in vitro Cu-induced LDL oxidation under the study conditions.

show abstract

The effects of free radical scavengers on the oxidation of low-density lipoproteins by macrophages

Cited by 10 publications

References 39 publications

Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions. A possible peroxidative role for paraoxonase.

Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions. A possible peroxidative role for paraoxonase.

The evaluation of the oxidative state of native-LDL: three methods compared

Effect of zinc supplementation on in vitro copper-induced oxidation of low-density lipoproteins in healthy French subjects aged 55–70 years:the Zenith Study

Contact Info

Product

Resources

About