The effects of fluid shear stress on proliferation and osteogenesis of human periodontal ligament cells

Zheng, Lisha; Chen, Luoping; Chen, Yuchao; Gui, Jinpeng; Li, Qing; Huang, Yan; Liu, Meili; Jia, Xiaolin; Song, Wei; Ji, Jing; Gong, Xianghui; Shi, Ruoshi; Fan, Yubo

doi:10.1016/j.jbiomech.2016.01.034

Cited by 33 publications

(34 citation statements)

References 40 publications

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“…7 Suppressing marrow adipogenesis with a nonpharmaceutical strategy that also affects other organs and systems positively is plausible, yet the information on which constituents of a mechanical signal is sensed and responded by bone marrow stem cells is still limited. 18,19 Bone cells sense and respond to different forms of mechanical loads, including cyclic stretch, 20 static pressure, 21 shear stress 22,23 and nanoscale mechanotransduction. 24,25 In addition to these loading variants, daily application of low-intensity ( \ 0.5 g, 1 g = 9.81 m/s 2 ) vibrations (LIVs) in high frequencies ( .…”

Section: Introductionmentioning

confidence: 99%

“…Bone cells sense and respond to different forms of mechanical loads, including cyclic stretch, 20 static pressure, 21 shear stress 22,23 and nanoscale mechanotransduction. 24,25 In addition to these loading variants, daily application of low-intensity (<0.5 g, 1 g = 9.81 m/s 2 ) vibrations (LIVs) in high frequencies (>30 Hz) is anabolic to bone tissue and can suppress obesity in murine and clinical studies.…”

Section: Introductionmentioning

confidence: 99%

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Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells

Baskan

Meşe

Özçivici

2017

Proc Inst Mech Eng H

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Bone marrow mesenchymal stem cells that are committed to adipogenesis were exposed daily to high-frequency low-intensity mechanical vibrations to understand molecular, morphological and ultrastructural adaptations to mechanical signals during adipogenesis. D1-ORL-UVA mouse bone marrow mesenchymal stem cells were cultured with either growth or adipogenic medium for 1 week. Low-intensity vibration signals (15 min/day, 90 Hz, 0.1 g) were applied to one group of adipogenic cells, while the other adipogenic group served as a sham control. Cellular viability, lipid accumulation, ultrastructure and morphology were determined with MTT, Oil-Red-O staining, phalloidin staining and atomic force microscopy. Semiquantitative reverse transcription polymerase chain reaction showed expression profile of the genes responsible for adipogenesis and ultrastructure of cells. Low-intensity vibration signals increased viability of the cells in adipogenic culture that was reduced significantly compared to quiescent controls. Low-intensity vibration signals also normalized the effects of adipogenic condition on cell morphology, including area, perimeter, circularization and actin cytoskeleton. Furthermore, low-intensity vibration signals reduced the expression of some adipogenic markers significantly. Mesenchymal stem cells are sensitive and responsive to mechanical loads, but debilitating conditions such as aging or obesity may steer mesenchymal stem cells toward adipogenesis. Here, daily application of low-intensity vibration signals partially neutralized the effects of adipogenic induction on mesenchymal stem cells, suggesting that these signals may provide an alternative and/or complementary option to reduce fat deposition.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells

Baskan

Meşe

Özçivici

2017

Proc Inst Mech Eng H

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“… 191 The mineralized matrix deposition is also increased by increasing FSS. 192 – 194 Further studies have revealed that dynamic fluid flow with higher peak shear stress amplitudes, faster oscillating frequencies, and longer loading durations provide the best conditions for promoting bone formation. 195 It has been shown that tissues with higher modulus increase the accumulation rate of d -aspartic acid, which is positively correlated with the half-life of collagen 196 and thereby could influence the collagen properties.…”

Section: Insoluble Cuesmentioning

confidence: 99%

Biomimetic delivery of signals for bone tissue engineering

et al. 2018

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Bone tissue engineering is an exciting approach to directly repair bone defects or engineer bone tissue for transplantation. Biomaterials play a pivotal role in providing a template and extracellular environment to support regenerative cells and promote tissue regeneration. A variety of signaling cues have been identified to regulate cellular activity, tissue development, and the healing process. Numerous studies and trials have shown the promise of tissue engineering, but successful translations of bone tissue engineering research into clinical applications have been limited, due in part to a lack of optimal delivery systems for these signals. Biomedical engineers are therefore highly motivated to develop biomimetic drug delivery systems, which benefit from mimicking signaling molecule release or presentation by the native extracellular matrix during development or the natural healing process. Engineered biomimetic drug delivery systems aim to provide control over the location, timing, and release kinetics of the signal molecules according to the drug’s physiochemical properties and specific biological mechanisms. This article reviews biomimetic strategies in signaling delivery for bone tissue engineering, with a focus on delivery systems rather than specific molecules. Both fundamental considerations and specific design strategies are discussed with examples of recent research progress, demonstrating the significance and potential of biomimetic delivery systems for bone tissue engineering.

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“…Zheng et. al demonstrated a negative effect of mechanical forces on the proliferation and migration of cells 53 . Previous studies report the manipulation of MNPs using external magnetic fields to exert rotating-oscillating motions or a simple magnetic pull downwards with a static magnet 54,55 which caused cellular mechanotrasduction.…”

Section: Chemical and Mechanical Stimulimentioning

confidence: 99%

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

Narayanasamy

Price

Merkhan

et al. 2019

Preprint

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The biocompatibility of surface coated/functionalised magnetic nanoparticles (MNPs) is key to their successful incorporation and application in biological systems. Polyethylene imine (PEI) -coated MNPs provide improved in vitro transfection efficiency compared to conventional chemical methods such as Lipofectamine and cationic polymers, and are also safer than viral transduction. Commercial cell toxicity assays are useful for end-point and high-throughput screening, providing fast results and an overview of cell health. However these assays only take into account cells that have undergone an extreme toxic response leading to cell death. Cell toxicity is a complex process which can be expressed in many forms, through morphological, metabolic, and epigenetic changes. A common indicator of cell stress and toxic response is increased cell adhesion and stress fibre formation. It is important to identify these changes in cells as it may affect downstream results and applications in biomedicine. This study explores the effect of the nanomagnetic transfection agent PEI-coated MNPs (MNP-PEIs) and an external magnetic field on cell behaviour, by studying particle internalization, changes in cellular morphology, and cell adhesion. We found that MNP-PEIs induced cell stress through a dose-dependent increase in cell adhesion via the overexpression of vinculin and formation of actin stress fibres. While the presence of PEI was the main contributor to increased cell stress, free PEI polyplexes induced higher toxicity compared to PEI bound to MNPs. MNPs without PEI coating however did not adversely affect cells suggesting a chemical effect instead of a mechanical one. In addition, genes identified as being associated with actin fibre regulation and cell adhesion, showed significant increases in expression due to the internalization of the MNP-PEI complex. From these results, we identify anomalous cell behaviour, morphology, and gene expression after interaction with MNP-PEIs, as well as a safe dosage to reduce acute cell toxicity.

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The effects of fluid shear stress on proliferation and osteogenesis of human periodontal ligament cells

Cited by 33 publications

References 40 publications

Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells

Low-intensity vibrations normalize adipogenesis-induced morphological and molecular changes of adult mesenchymal stem cells

Biomimetic delivery of signals for bone tissue engineering

Effect of PEI-coated MNPs on the Regulation of Cellular Focal Adhesions and Actin Stress Fibres

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