The effects of fatty acid composition on cardiac hypertrophy and function in mouse models of diet-induced obesity

Nguyen, Son; Shao, Dan; Tomasi, Loreta Casquel de; Braun, Alyssa N; Mattos, Ana Barbosa Marcondes de; Choi, Yong Seon; Villet, Outi; Roe, Nathan D.; Halterman, Carliana R.; Tian, Rong; Kolwicz, Stephen C.

doi:10.1016/j.jnutbio.2017.05.009

Cited by 22 publications

(21 citation statements)

References 23 publications

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“…One of the obesity hallmarks is the increase in oxidative stress and inflammation [68]. However, we did not observe differences in lipid peroxidation, nor in protein carbonylation between the HFD and SD groups, which may be attributed to the composition of the mouse chow used [72] or the duration of our study [50]. Similarly, the protein carbonyl content in heart tissue did not differ between SD and HFD mice.…”

Section: Discussionmentioning

confidence: 48%

“…Opinion on the extent of the detrimental effect of HFD in the heart differs. Several studies imply that an HFD diet alone is not sufficient for inducing heart failure [44,70,71], while others report lipotoxic cardiomyopathy under long dietary regimens [49,50] or cardiac hypertrophy under specific FA contents of a HFD [72]. HFD treatment is also one of the established models for the induction of aortic valve disease [73][74][75].…”

Section: Discussionmentioning

confidence: 99%

“…Although there are some studies investigating the effects of dietary fat on rodent cardiac functions, most of them do not report specific FA profiles or do so only for total lipid FA profiles in heart tissue [50,53,72]. Alternatively, only FA profiles for one selected group of lipid species are presented [86][87][88].…”

Section: Discussionmentioning

confidence: 99%

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The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

et al. 2020

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The Western diet can lead to alterations in cardiac function and increase cardiovascular risk, which can be reproduced in animal models by implementing a high-fat diet (HFD). However, the mechanism of these alterations is not fully understood and may be dependent on alterations in heart lipid composition. The aim of this study was to evaluate the effect of an HFD on the fatty acid (FA) composition of total lipids, as well as of various lipid fractions in the heart, and on heart function. C57BL/6 mice were fed an HFD or standard laboratory diet. The FA composition of chow, serum, heart and skeletal muscle tissues was measured by gas chromatography–mass spectrometry. Cardiac function was evaluated by ultrasonography. Our results showed an unexpected increase in polyunsaturated FAs (PUFAs) and a significant decrease in monounsaturated FAs (MUFAs) in the heart tissue of mice fed the HFD. For comparison, no such effects were observed in skeletal muscle or serum samples. Furthermore, we found that the largest increase in PUFAs was in the sphingolipid fraction, whereas the largest decrease in MUFAs was in the phospholipid and sphingomyelin fractions. The hearts of mice fed an HFD had an increased content of triacylglycerols. Moreover, the HFD treatment altered aortic flow pattern. We did not find significant changes in heart mass or oxidative stress markers between mice fed the HFD and standard diet. The above results suggest that alterations in FA composition in the heart may contribute to deterioration of heart function. A possible mechanism of this phenomenon is the alteration of sphingolipids and phospholipids in the fatty acid profile, which may change the physical properties of these lipids. Since phospho- and sphingolipids are the major components of cell membranes, alterations in their structures in heart cells can result in changes in cell membrane properties.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

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The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

et al. 2020

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“…In accordance with previous studies, mice fed a high-fat diet (HFD) for 20 weeks were in a prediabetic state with obesity, hyperlipidaemia and insulin resistance, but without hyperglycaemia. In most studies, long-term high-fat feeding will increase left ventricular (LV) mass and induce LV concentric hypertrophy [ 17 ; 30 – 34 ]. The reported incidence of systolic and diastolic dysfunction is, however, inconsistent.…”

Section: Discussionmentioning

confidence: 99%

Isolated perfused working hearts provide valuable additional information during phenotypic assessment of the diabetic mouse heart

et al. 2018

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Although murine models for studying the development of cardiac dysfunction in diabetes mellitus are well established, their reported cardiac phenotypes vary. These reported divergences may, in addition to the severity of different models, also be linked to the methods used for cardiac functional assessment. In the present study, we examined the functional changes using conventional transthoracic echocardiography (in vivo) and isolated heart perfusion techniques (ex vivo), in hearts from two mouse models; one with an overt type 2 diabetes (the db/db mouse) and one with a prediabetic state, where obesity was induced by a high-fat diet (HFD). Analysis of left ventricular function in the isolated working hearts from HFD-fed mice, suggested that these hearts develop diastolic dysfunction with preserved systolic function. Accordingly, in vivo examination demonstrated maintained systolic function, but we did not find parameters of diastolic function to be altered. In db/db mice, ex vivo working hearts showed both diastolic and systolic dysfunction. Although in vivo functional assessment revealed signs of diastolic dysfunction, the hearts did not display reduced systolic function. The contrasting results between ex vivo and in vivo function could be due to systemic changes that may sustain in vivo function, or a lack of sensitivity using conventional transthoracic echocardiography. Thus, this study demonstrates that the isolated perfused working heart preparation provides unique additional information related to the development of cardiomyopathy, which might otherwise go unnoticed when only using conventional echocardiographic assessment.

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“…These findings reveal the importance of choosing the method in the cardiac functional outcome. Among the studies that employed WD with high carbohydrate content, authors did not show cardiac dysfunction by echocardiographic or hemodynamic evaluation, independently of experimental protocol duration [39][40][41]. Conversely, authors evidenced the presence of relevant systolic and diastolic dysfunction, evaluated by echocardiogram, when the WD high in carbohydrate was associated with 25% fructose or sucrose in drinking water, suggesting a crucial role of sugars [14,[42][43][44][45].…”

Section: Discussionmentioning

confidence: 97%

Temporal Measures in Cardiac Structure and Function During the Development of Obesity Induced by Different Types of Western Diet in a Rat Model

et al. 2019

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Obesity is recognized worldwide as a complex metabolic disorder that has reached epidemic proportions and is often associated with a high incidence of cardiovascular diseases. To study this pathology and evaluate cardiac function, several models of diet-induced obesity (DIO) have been developed. The Western diet (WD) is one of the most widely used models; however, variations in diet composition and time period of the experimental protocol make comparisons challenging. Thus, this study aimed to evaluate the effects of two different types of Western diet on cardiac remodeling in obese rats with sequential analyses during a long-term follow-up. Male Wistar rats were distributed into three groups fed with control diet (CD), Western diet fat (WDF), and Western diet sugar (WDS) for 41 weeks. The animal nutritional profile and cardiac histology were assessed at the 41st week. Cardiac structure and function were evaluated by echocardiogram at four different moments: 17, 25, 33, and 41 weeks. A noninvasive method was performed to assess systolic blood pressure at the 33rd and 41st week. The animals fed with WD (WDF and WDS) developed pronounced obesity with an average increase of 86.5% in adiposity index at the end of the experiment. WDF and WDS groups also presented hypertension. The echocardiographic data showed no structural differences among the three groups, but WDF animals presented decreased endocardial fractional shortening and ejection fraction at the 33rd and 41st week, suggesting altered systolic function. Moreover, WDF and WFS animals did not present hypertrophy and interstitial collagen accumulation in the left ventricle. In conclusion, both WD were effective in triggering severe obesity in rats; however, only the WDF induced mild cardiac dysfunction after long-term diet exposure. Further studies are needed to search for an appropriate DIO model with relevant cardiac remodeling.

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The effects of fatty acid composition on cardiac hypertrophy and function in mouse models of diet-induced obesity

Cited by 22 publications

References 23 publications

The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

Isolated perfused working hearts provide valuable additional information during phenotypic assessment of the diabetic mouse heart

Temporal Measures in Cardiac Structure and Function During the Development of Obesity Induced by Different Types of Western Diet in a Rat Model

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