The effects of family, dentition, and dental caries on the salivary microbiome

Foxman, Betsy; Luo, Ting; Srinivasan, Usha; Ramadugu, Kirtana; Wen, Ai; Goldberg, Deborah E.; Shedden, Kerby; Crout, Richard J.; McNeil, Daniel W.; Weyant, Robert J.; Marazita, Mary L.

doi:10.1016/j.annepidem.2016.03.006

Cited by 23 publications

(21 citation statements)

References 31 publications

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“…Furthermore, few studies included young adults as subjects. A cross-sectional study exploring salivary microbiomes among families, including young adults, reported that dental caries did not impact microbial diversity [28]. While our findings support the results of this study, further cohort studies are required to investigate the relationship between microbiome diversity and an increase in dental caries.…”

Section: Discussionsupporting

confidence: 78%

Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study

Uchida-Fukuhara

Ekuni

Islam

et al. 2020

IJERPH

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The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (Δdecayed, missing, and filled teeth (ΔDMFT) score increased during the 3-year period) and non-increased group (ΔDMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group (n = 14) was similar to that in the non-increased group (n = 41). Relative abundances of the family Prevotellaceae (p = 0.007) and genera Alloprevotella (p = 0.007) and Dialister (p = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.

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Section: Discussionsupporting

confidence: 78%

Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study

Uchida-Fukuhara

Ekuni

Islam

et al. 2020

IJERPH

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“…While the diversity did not dramatically change from 1 week to 8 weeks and again to 1 year, there were significant compositional changes which signify the role of factors, such as diet, dentition, salivary flow rate, oral health, siblings etc., have to play. We did however observe a lower diversity at all ages in the infant oral microbiota compared to the maternal 'adult' salivary microbiota, with similar compositional differences which have been identified previously in the salivary microbiome between adult and infant [64][65][66]. The diversity gap between the mother and infant oral microbiota increased as the infant grew in age demonstrating closer relatedness at a younger age.…”

Section: Discussionsupporting

confidence: 84%

The microbiota of the mother at birth and its influence on the emerging infant oral microbiota from birth to 1 year of age: a cohort study

Hurley

Mullins

Barrett

et al. 2019

Journal of Oral Microbiology

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Background: The acquisition of microbial communities and the influence of delivery mode on the oral microbiota of the newborn infant remains poorly characterised. Methods: A cohort of pregnant women were enrolled in the study (n = 84). All infants were born full term, by Spontaneous vaginal delivery (SVD) or by Caesarean section (CS). At delivery a saliva sample along with a vaginal/skin sample from the mother. Saliva samples were the taken from the infant within one week of birth, and at week 4, week 8, 6 months and 1 year of age. We used high-throughput sequencing of V4-V5 region 16S rRNA amplicons to compare the microbiota of all samples. Results: The vaginal microbiota had a lower alpha diversity than the skin microbiota of the mother, while the infant oral microbiota diversity remained relatively stable from birth to 8 weeks of age. The oral microbiota of the neonate differed by birth modality up to 1 week of age (p < 0.05), but birth modality did not have any influence on the infant oral microbiota beyond this age. Conclusions: We conclude thatbirth mode does not have an effect on the infant oral microbiota beyond 4 weeks of age, and the oral microbiota of infants continues to develop until 1 year of age.

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“…For example, although the volunteers were all healthy at the time of saliva collection, it is possible that undetected differences in oral health might be influencing bacterial diversity. However, a recent study found a negligible impact of dental caries on the salivary microbiome [ 43 ]. Focusing on only a portion of the 16S rRNA gene (in order to utilize the NGS capacity) instead of analyzing the entire gene could also limit our ability to assign the sequences to different species, as reported previously [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Unraveling the human salivary microbiome diversity in Indian populations

2017

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The importance of studying the salivary microbiome has been highlighted for its connection to health and disease and as a potential tool for supplementing human genetic diversity studies. While the salivary microbiome has been studied in various world populations, Indian populations have not been examined. We therefore analyzed microbiome diversity in saliva samples from 92 volunteers from eight different sampling locations in India by amplifying and sequencing variable regions (V1 and V2) of the bacterial 16S rRNA gene. The results showed immense bacterial richness in Indian populations; we identified 165 bacterial genera and 785 unique Operational Taxonomic Units (OTUs), with substantial sharing among the populations. There were small, but significant correlations in the abundance of bacterial genera in sampling locations from the same geographic region. Most of the core OTUs detected were also observed previously in other populations, but Solobacterium spp., Lachnoanaerobaculum spp. and Alloprevotella spp. were observed to be a component of the saliva microbiome unique to Indian populations. Importantly, nine bacterial genera were observed that were not listed in the Human Oral Microbiome Database (HOMD). These results highlight the importance of analyzing underrepresented populations like those of India.

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The effects of family, dentition, and dental caries on the salivary microbiome

Cited by 23 publications

References 31 publications

Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study

Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study

The microbiota of the mother at birth and its influence on the emerging infant oral microbiota from birth to 1 year of age: a cohort study

Unraveling the human salivary microbiome diversity in Indian populations

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