The effects of examination stress on salivary cortisol, immunoglobulin A, and chromogranin A in nursing students

Takatsuji, Koichi; Sugimoto, Yukihiko; Ishizaki, Shoko; Ozaki, Yasuka; Matsuyama, Etsuko; Yamaguchi, Yukari

doi:10.2220/biomedres.29.221

Cited by 90 publications

(73 citation statements)

References 17 publications

(19 reference statements)

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“…When the acute stress model was applied in our study, significant increases at 15 min were obtained in both salivary cortisol, as reported before (Geverink et al, 2002;Merlot et al, 2011), and CgA concentrations. The mean increase of CgA was of 31%, a value that is between the increases described in several human studies about acute stress for the effect of examination (26%; Takatsuji et al, 2008) and after venipuncture in children (49%; Lee et al, 2006). Moreover, in the study about social isolation stress using saliva sampling in cows was described as a mean increase of 50% after 30 min of social isolation (Ninomiya and Sato, 2011).…”

Section: Discussionsupporting

confidence: 51%

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Escribano

Soler

Gutiérrez

et al. 2013

Animal

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The objective of this study was to develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for porcine salivary chromogranin A (CgA) measurements, using a species-specific antibody, and evaluate its behaviour in an acute stress model. Polyclonal antibodies were produced in rabbits immunized with a synthetic porcine fragment of CgA 3592379 and used to develop a sandwich TR-IFMA. This TR-IFMA was analytically validated and showed intra-and inter-assay coefficients of variation of 6.23% and 5.82%, respectively, an analytical limit of detection of 4.27 3 10 23 mg/ml and a limit of quantification of 24.5 3 10 23 mg/ml. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution (r 5 0.975) and recovery tests. When a model of experimental acute stress, in which animals were immobilized for 3 min with a nose snare (stressor stimulus), was applied, a significant increase (P , 0.05) in CgA levels in saliva was detected at 15 min post-stressor stimulus. These results indicate that the assay developed in this study could measure CgA in porcine saliva in a reliable way and that the concentrations of CgA in saliva samples of pigs increase after an acute stress situation.

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Section: Discussionsupporting

confidence: 51%

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Escribano

Soler

Gutiérrez

et al. 2013

Animal

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“…11 Salivary CgA, thus, has been proposed as a surrogate marker of sympathetic nervous system activity. 12 A series of studies has shown that compared with salivary cortisol, salivary CgA increases more rapidly and more sensitively to psychological stressors induced by activities such as public speaking, 12 word-processing and arithmetic tasks, 13,14 academic examinations, 15 and venipuncture in hospitalized children. 16 A recent finding from the author's laboratory further demonstrated that salivary CgA levels in the late-luteal phase significantly increased among women with severe premenstrual emotional symptoms, while salivary cortisol showed no menstrual-cyclic changes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Olfactory Stimulation from the Fragrance of the Japanese Citrus Fruit Yuzu (Citrus junos Sieb. ex Tanaka) on Mood States and Salivary Chromogranin A as an Endocrinologic Stress Marker

Matsumoto

Asakura²,

Hayashi

2014

The Journal of Alternative and Complementary Medicine

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Objective: This study investigated the soothing effects of fragrance from yuzu, a Japanese citrus fruit (Citrus junos Sieb. ex Tanaka), with salivary chromogranin A (CgA) used as an endocrinologic stress marker reflecting sympathetic nervous system activity. Methods: Twenty healthy women (mean age, 20.5 -0.1 years) participated in a randomized, controlled, crossover study. Participants were examined on two separate occasions-once using the yuzu scent and once using unscented water as a control-in the follicular phase. This experiment measured salivary CgA and the Profile of Mood States (POMS) as a psychological index before and after the aromatic stimulation. Results: Ten-minute inhalation of the yuzu scent significantly decreased salivary CgA. At 30 minutes after the inhalation period, the salivary CgA level further decreased. In addition, POMS revealed that inhalation of the aromatic yuzu oil significantly decreased total mood disturbance, a global measure of affective state, as well as four subscores of emotional symptoms (tension-anxiety, depression-dejection, anger-hostility, and confusion), as long as 30 minutes after the olfactory stimulation. Conclusions: Yuzu's aromatic effects may alleviate negative emotional stress, which, at least in part, would contribute to the suppression of sympathetic nervous system activity.

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“…Results revealed that the concentrations of IgA and CgA presented a statistically significant increase immediately after the exam had finished and decreased after two hours. No significant differences were observed in the salivary cortisol concentration in the period covering 1 hour before the exam, immediately after it and 2 hours after it (26) . In a study that correlated stress and infectious diseases, a positive association was found between the common cold (61.7%) and herpes (21.3%).…”

Section: Discussionmentioning

confidence: 72%

“…Some (17,(19)(20)(22)(23)(25)(26) analysed the relationship between stress and physiological or psychological manifestations, and others (10,12,21,24,(27)(28) explored the general health state of the students involved in the researches.…”

Section: Resultsmentioning

confidence: 99%

Respostas fisiológicas e emocionais ao estresse em estudantes de enfermagem: revisão integrativa da literatura científica

Benavente¹,

Costa

2011

Acta paul. enferm.

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Objective: To analyze the scientific results published in national and international nursing journals related to the physiological and emotional responses of nursing students. Methods: We performed an integrative review of scientific literature, focusing on the databases PUBMED, MEDLINE, CINAHL, LILACS and SciELO. In the sample, we included works available in full text, in Portuguese, English or Spanish, published between July 2004 to July 2009, and which contained at least one descriptor in the title and three in the text, directly related to physiological and psychological manifestations of stress in nursing students. Results: Of 126 articles identified, only 13 referred to the theme; of these, five addressed psychological manifestations, four addressed physiological manifestations, and four others identified both. Of the total, four studies were longitudinal and nine were transverse. Conclusion: In terms of results, it was observed that the theme should be further studied and developed in the process of teaching and learning of nursing, because it was verified that stress is a frequent occurrence and has major consequences for these students. Descriptors: Nursing student/psychology; Stress; Physiological stress; Psychological stress RESUMO Objetivo: Analisar a produção científica relacionada às respostas fisiológicas e emocionais em estudantes de enfermagem, registrada nos periódicos nacionais e internacionais de enfermagem. Métodos: Foi realizada uma revisão integrativa da literatura científica, tendo como foco as bases de dados PUBMED, MEDLINE, CINAHL, LILACS e SciELO. Na amostra, foram incluídos trabalhos escritos na íntegra, em português, inglês ou espanhol, no período de julho de 2004 a julho de 2009, que continham, pelo menos, um descritor no título e três no texto, diretamente relacionados a manifestações fisiológicas e psicológicas do estresse em estudantes de enfermagem. Resultados: Dos 126 artigos identificados apenas 13 referiram-se ao tema, deles, cinco abordaram manifestações psicológicas, quatro, manifestações fisiológicas e outros quatro ambas. Do total, quatro foram estudos longitudinais e nove transversais. Conclusão: Pelos resultados atingidos, observou-se que o tema ainda deve ser estudado e desenvolvido no processo de ensino e aprendizagem da enfermagem, pois verificou-se que o estresse é uma ocorrência frequente e com consequências importantes entre esses estudantes. Descritores: Estudante de enfermagem/psicologia; Estresse; Estresse fisiológico; Estresse psicológico RESUMEN Objetivo: Analizar la producción científica relacionada a las respuestas fisiológicas y emocionales de estudiantes de enfermería, registrada en los periódicos nacionales e internacionales de enfermería. Métodos: Se realizó una revisión integrativa de la literatura científica, teniendo como foco las bases de datos PUBMED, MEDLINE, CINAHL, LILACS y SciELO. En la muestra, fueron incluídos trabajos escritos íntegramente, en portugués, inglés o español, en el período de julio del 2004 a julio del 2009, que contenía...

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The effects of examination stress on salivary cortisol, immunoglobulin A, and chromogranin A in nursing students

Cited by 90 publications

References 17 publications

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Measurement of chromogranin A in porcine saliva: validation of a time-resolved immunofluorometric assay and evaluation of its application as a marker of acute stress

Effects of Olfactory Stimulation from the Fragrance of the Japanese Citrus Fruit Yuzu (Citrus junos Sieb. ex Tanaka) on Mood States and Salivary Chromogranin A as an Endocrinologic Stress Marker

Respostas fisiológicas e emocionais ao estresse em estudantes de enfermagem: revisão integrativa da literatura científica

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