The Effects of Ethanol on Glucose 6-Phosphate Dehydrogenase Enzyme Activity From Human Erythrocytes in Vitro and Rat Erythrocytes in Vivo

Büyükokuroğlu, Mehmet Emin; Altıkat, Sayit; Çiftçi, Mehmet

doi:10.1093/alcalc/37.4.327

Cited by 15 publications

(11 citation statements)

References 16 publications

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“…The major role of NADPH in erythrocytes is the regeneration of reduced glutathione, which in turn prevents heme oxidation and preserves the integrity of sulphydryl groups of α and β chains to forestall Heinz bodies and the integrity of red blood cell membrane proteins [35]. G6PDH activity is also influenced by many chemical agents and drugs, like ampicillin sulphate, Mg 2+ , diazepam and ethanol [27,[36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Chemical and Pathological Oxidative Influences on Band 3 Protein Anion-exchanger

Teti¹,

Maria²,

Busá³

et al. 2005

Cell Physiol Biochem

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Background/aims: The erythrocyte is a cell exposed to a high level of oxygen pressure and to oxidative chemical agents. This stress involves SH-groups oxidation, cell shrinkage by activation of K-Cl cotransport (KCC) and elevation of the band 3 tyrosine phosphorylation level. The aim of our study was to test whether oxidative stress could influence band 3-mediated anion transport in human red blood cells. Methods: To evaluate this hypothesis, normal and pathological (glucose 6 phosphate dehydrogenase (G6PDH) defficient) erythrocytes were treated with known sulphydryl-blocking or thiol-oxidizing agents, such as N-ethylmaleimide (NEM), azodicarboxylic acid bis[dimethylamide] (diamide), orthovanadate, Mg 2+ and tested for sulphate (SO 4 -) uptake, K + efflux, G6PDH activity and glutathione (GSH) concentration. Results: In normal red blood cells, the rate constants of SO 4 -uptake decreased by about 28 % when cells were incubated with NEM, diamide and orthovanadate. In G6PDH-deficient red blood cells, in which oxidative stress occurs naturally, the rate constant of sulphate uptake was decreased by about 40% that of normal red cells. Addition of oxidizing and phosphatase inhibitor agents to pathological erythrocytes further decreased anion transport. In contrast, G6PDH activity was increased under oxidative stress in normal as well as in pathological cells and was lower in the presence of exogenous Mg 2+

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Section: Introductionmentioning

confidence: 99%

Chemical and Pathological Oxidative Influences on Band 3 Protein Anion-exchanger

Teti¹,

Maria²,

Busá³

et al. 2005

Cell Physiol Biochem

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“…In previous research, it was discovered that while abscisic acid increased the rat erythrocyte G6PD enzyme activity, it decreased the enzyme activity of putrescine, metamizole, magnesium sulfate, ethanol, dantrolene sodium, naringenin, and lead acetate . It was also determined that while dantrolene sodium, propofol, and lead acetate decreased 6PGD enzyme activities, naringenin had no significant effect on enzyme activity …”

Section: Discussionmentioning

confidence: 98%

Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats

Temel

Bengü

Akkoyun

et al. 2017

J Biochem & Molecular Tox

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In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2',5'-ADP-Sepharose 4B affinity gel. The effects of Ast and Al ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al inhibited the enzyme activity noncompetitively (IC values; 0.679 mM, K values 1.32 mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al + Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group (P < 0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al + Ast groups (P < 0.05).

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“…investigated in vitro effect of new oxindoles derivatives on rat erythrocyte G6PD and 6PGD enzymes activity. Another study deals with effect of ethanol on rat and human erythrocyte G6PD enzyme activity . Temel and Kocyigit investigated the effect of various metal ions on rat erythrocyte G6PD enzyme activity.…”

Section: Discussionmentioning

confidence: 99%

The effect of astaxanthin and cadmium on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzymes activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro

Akkoyun

Bengü²,

Temel

et al. 2018

J Biochem & Molecular Tox

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In this study, the effects of astaxanthin (AST) that belongs to carotenoid family and cadmium (Cd), which is an important heavy metal, on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzyme activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro were studied. In in vitro studies, 6PGD enzyme was purified from rat erythrocytes with 2',5'-ADP Sepharose4B affinity chromatography. Results showed inhibition of enzyme by Cd at IC ; 346.5 μM value and increase of 6PGD enzyme activity by AST. In vivo studies showed an increase in G6PD, 6PGD, and GR enzyme activities (P ˃ 0.05) and no chance in TrxR enzyme activity by AST. Cd ion inhibited G6PD, 6PGD, and GR enzyme activities (P ˂ 0.05) and also decreased TrxR enzyme activity (P ˃ 0.05). AST + Cd group G6PD enzyme activity was statistically low compared with control group (P ˂ 0.05). 6PGD and TrxR enzyme activities decreased without statistical significance (P ˃ 0.05); however, GR enzyme activity increased statistically significantly (P ˂ 0.05).

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The Effects of Ethanol on Glucose 6-Phosphate Dehydrogenase Enzyme Activity From Human Erythrocytes in Vitro and Rat Erythrocytes in Vivo

Abstract: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro.

Cited by 15 publications

References 16 publications

Chemical and Pathological Oxidative Influences on Band 3 Protein Anion-exchanger

Chemical and Pathological Oxidative Influences on Band 3 Protein Anion-exchanger

Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats

The effect of astaxanthin and cadmium on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzymes activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro

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