The Effects of Ergogenic Compounds on Myogenic Satellite Cells

Vierck, Janet L.; Icenoggle, Deri L.; Bucci, Luke R.; Dodson, Michael V.

doi:10.1249/01.mss.0000065005.96298.01

Cited by 55 publications

(41 citation statements)

References 32 publications

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“…CrM supplementation enhances satellite cell differentiation in vitro (93) and satellite cell mitotic activity during compensatory hypertrophy in rat skeletal muscle (16). These observations are consistent with the results from an in vivo study showing an increase in the protein content of the myogenic transcription factors myogenic regulatory factor-4 (MRF4) and myogenin after recovery from cast-induced atrophy in the skeletal muscle of young subjects supplemented with CrM (36).…”

supporting

confidence: 86%

“…In the present study, we did not observe an increase in mean fiber area as previously reported, which could be because of our short period of supplementation compared with longer periods in studies showing this effect (94). Nonetheless, we did observe an early transcriptional and translational response at the cellular level, which explains previously observed effects of creatine supplementation on satellite cell proliferation and differentiation (16,36,41,63,93).…”

Section: Crm May Promote Satellite Cell Proliferation and Differentiasupporting

confidence: 62%

“…In vitro and in vivo, CrM supplementation alone (41,93) or in combination with progressive strength training or increased functional loading and compensatory hypertrophy (synergist ablation) (16) induces satellite cell (postnatal myogenic stem cells) proliferation and differentiation. This effect may be mediated, at least in part, by CrM-induced cell swelling, an early cellular response to CrM supplementation.…”

Section: Crm May Promote Satellite Cell Proliferation and Differentiamentioning

confidence: 99%

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Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation

Safdar

Yardley

Snow

et al. 2008

Physiological Genomics

121

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Safdar A, Yardley NJ, Snow R, Melov S, Tarnopolsky MA. Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation. Physiol Genomics 32: 219-228, 2008. First published October 23, 2007 doi:10.1152/physiolgenomics.00157.2007.-Creatine monohydrate (CrM) supplementation has been shown to increase fat-free mass and muscle power output possibly via cell swelling. Little is known about the cellular response to CrM. We investigated the effect of short-term CrM supplementation on global and targeted mRNA expression and protein content in human skeletal muscle. In a randomized, placebocontrolled, crossover, double-blind design, 12 young, healthy, nonobese men were supplemented with either a placebo (PL) or CrM (loading phase, 20 g/day ϫ 3 days; maintenance phase, 5 g/day ϫ 7 days) for 10 days. Following a 28-day washout period, subjects were put on the alternate supplementation for 10 days. Muscle biopsies of the vastus lateralis were obtained and were assessed for mRNA expression (cDNA microarrays ϩ real-time PCR) and protein content (Kinetworks KPKS 1.0 Protein Kinase screen). CrM supplementation significantly increased fat-free mass, total body water, and body weight of the participants (P Ͻ 0.05). Also, CrM supplementation significantly upregulated (1.3-to 5.0-fold) the mRNA content of genes and protein content of kinases involved in osmosensing and signal transduction, cytoskeleton remodeling, protein and glycogen synthesis regulation, satellite cell proliferation and differentiation, DNA replication and repair, RNA transcription control, and cell survival. We are the first to report this large-scale gene expression in the skeletal muscle with short-term CrM supplementation, a response that suggests changes in cellular osmolarity. ergogenic aid; osmosensing; cell signaling; cDNA microarray; realtime PCR CREATINE MONOHYDRATE (CrM) supplementation has a number of biochemical and physiological effects and enhances muscle performance in humans (89). Intracellular phosphocreatine (PCr) functions as an energy buffer to prevent ATP depletion in the skeletal muscle, especially during short-duration repetitive high-intensity exercise bouts (22,43,75,89). Following the intake of 20 g CrM/day for 4 -7 days (2, 29, 33) or 3 g CrM/day for 4 -12 wk (4,46,91,94), skeletal muscle total creatine and PCr increase by 10 -20%. Short-term CrM supplementation increases muscle force and/or power (2,3,7,8,10,18,29,59,96), whereas chronic CrM supplementation in conjunction with weight training increases maximal muscle strength and power, fat-free mass (FFM), muscle fiber size, total body water, and total body weight (47, 91, 94) compared with placebo. The increase in FFM and total body weight is partly due to fluid retention in myocytes caused by the osmotic potential of high intracellular CrM abundance (45,47,60). Whether the aforementioned phenotypic effects are due to energy buffering, physiochemical attributes of the compound, cell volume regulation, ...

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supporting

confidence: 86%

Section: Crm May Promote Satellite Cell Proliferation and Differentiasupporting

confidence: 62%

Section: Crm May Promote Satellite Cell Proliferation and Differentiamentioning

confidence: 99%

See 1 more Smart Citation

Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation

Safdar

Yardley

Snow

et al. 2008

Physiological Genomics

121

View full text Add to dashboard Cite

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“…In this study, the expression of IGF-I gene was significantly increased in leg muscle in both the Cr-Pyr and Cr groups, and this was accompanied by increased relative leg muscle weight. The previous studies indicated that Cr may increase differentiation and protein synthesis in ovine myogenic satellite cells (Vierck, et al, 2003) and primary myotubes from chick embryos (Ingwall et al, 1974). In addition, several human studies have shown that Cr increases muscle protein (Balsom et al, 1995;Willoughby and Rosene, 2001;Deldicque et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

Chen

Wang

Kong

et al. 2011

Animal

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The effects of pyruvate (Pyr), creatine pyruvate (Cr-Pyr) and creatine (Cr) on lipid and protein metabolism were compared in broiler chickens. A total of 400 1-day-old male birds (Aconred) were allocated to four groups, each of which included four replicates (25 birds per replicate). Treatments consisted of unsupplemented basal diet (Control), basal diet containing 2% Pyr, basal diet containing 3% Cr and basal diet containing 5% Cr-Pyr. Cr-Pyr and Pyr significantly decreased the hepatic triglyceride and serum total cholesterol concentration (P , 0.01). Cr-Pyr markedly increased the serum non-esterified fatty acid and highdensity lipoprotein cholesterol concentrations (P , 0.05), whereas the expression of carnitine palmitoyl transferase I (P , 0.05) and peroxisome proliferators-activated receptor-a (P , 0.01) mRNA in the liver were both decidedly enhanced in the Cr-Pyr group. The relative leg muscle weight was higher in the Cr-Pyr group than in the control group, whereas the serum uric acid content and hepatic glutamic-oxaloacetic transaminase activity were lower in the Cr-Pyr and Cr groups (P , 0.05), respectively. Muscle insulin-like growth factor I (P , 0.05) expression was enhanced, and the myostatin (P , 0.01) mRNA level was reduced in both the Cr-Pyr and Cr groups. In addition, Cr-Pyr did not alter body weight or the feed conversion ratio. These results indicate that, compared with Pyr and Cr alone, Cr-Pyr has a bifunctional role in broiler chickens, in that it influences both lipid and protein metabolism.Keywords: creatine pyruvate, pyruvate, creatine, lipolysis, protein synthesis ImplicationsIn this study, the administration of creatine pyruvate (Cr-Pyr, a new compound, which contains pure pyruvic acid molecularly bound to pure pharmaceutical grade creatine at a concentration ratio of 40 : 60) was compared with the administration of Pyr or Cr alone for its effect on lipid and protein metabolism in broilers. The insights gained from this study will provide a better understanding of the mechanisms involved in the Cr-Pyr effect on broiler chickens. Further clarity will help the industry to avoid obesity-related problems during production and explore new ways to improve muscle growth in broilers.

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“…Satellite cell fusion (4)(5)(6) with adjacent myofibers provides the nuclei required for regulating myofibrillar protein accretion (7), and the cellularity of these cells is required for replacing damaged myofibers (1). The history of the development of the ovine satellite cell system, in vitro, (1,(4)(5)(6)(7)(8)(9)(10), and the rationale for its use in the types of studies described, herein, have been reported (11)(12)(13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Nutritional Supplements on Muscle-Derived Stem Cells in vitro

Fernyhough¹,

Bucci²,

Feliciano³

et al. 2010

Int J Stem Cells

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Postnatal muscle stem cells, recognized as myogenic satellite cells, were isolated from sheep skeletal muscle and used in these experiments. Forty-one different metabolic compounds that are commonly found in commercially-available oral supplements were exposed to primary muscle stem cell cultures, in an effort to ascertain whether any one compound could alter satellite cell proliferation or differentiation (a first step towards elucidating the metabolomics or nutrigenomics of these stem cells). These compounds included energetic moieties, amino acid analogs, fatty acids and analogs including different forms of conjugated linoleic acid, minerals and mineral conjugates, insect hormones, caffeine, plant extracts, and extracts from over-the-counter supplements, and were obtained by key manufacturers in a form that would be commercially available. The compounds were sterilized and then exposed to myogenic satellite cell cultures at different levels (ranging from toxic to physiologic) to ascertain if there would be an effect. The results suggested that exposure of satellite cells to only a few compounds resulted in any measurable effect(s). Ten compounds elicited increases in proliferation, and four compounds promoted increases in differentiation. These results suggest avenues for the exploration of enhancing muscle stem cell activity of interest for muscle wasting disorders, sarcopenia of aging and physical performance.

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The Effects of Ergogenic Compounds on Myogenic Satellite Cells

Cited by 55 publications

References 32 publications

Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation

Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation

Comparison of the novel compounds creatine and pyruvateon lipid and protein metabolism in broiler chickens

The Effect of Nutritional Supplements on Muscle-Derived Stem Cells in vitro

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