The effects of environmental salinity on trunk kidney proteome of juvenile ayu (Plecoglossus altivelis)

Chen, Jiong; Wu, Hai Q.; Shi, Yu; Li, Chang H.; Li, Ming Y.

doi:10.1016/j.cbd.2009.06.003

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…The two studies provide one possible explanation of the higher expression level PtFer in some specific tissues which might disturb physiology leading to facilitate the ferroxidase activity of PtFer to store the released iron. Similar to our result, the ferritin mRNA of euryhaline teleost, Dicentrarchus labrax showed up-regulation in the intestine during the freshwater-acclimated conditions (Boutet et al, 2006), and the expression of ferritin in trunk kidney of juvenile ayu (Plecoglossus altivelis) was observed down-regulated when it was transferred from freshwater to brackish water (Chen et al, 2009).…”

Section: Homo Sapiens-l 45% To Mus Musculus-lsupporting

confidence: 89%

Untitled

Huang¹,

Xu²

2016

Turk. J. Fish. Aquat. Sci.

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Ferritin is a highly conserved iron storage protein playing an important role in the iron metabolism and cell protection. Swimming crab (Portunus trituberculatus) is an important fishery and aquaculture species in China and water salinity has a significant effect on its physiological processes. In order to verify whether the ferritin gene contributed to swimming crab salinity adaptation, ferritin (PtFer) cDNA open reading frame (ORF) was cloned. Homologous amino acid sequence alignment of PtFer showed a1 higher similarity to the ferritin heavy chain than the light chain. The gene expression profiles of PtFer under different salinity treatments were investigated by semi-quantitative RT-PCR. To further validate the salinity tolerance functions of PtFer, we investigated the eukaryotic expression of PtFer recombinant plasmid in 239T cells under a series of salinity stress. The results showed that the survival rate of the cells transfected with PtFer gene recombinant plasmid was significantly higher than that of the cells transfected with plasmid without insert fragment during the low salinity challenges, which indicated that PtFer might possess a protective effect against low salinity stress. Therefore, our results together indicated that the PtFer gene plays an important role in swimming crab salinity adaptation physiological process.

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Section: Homo Sapiens-l 45% To Mus Musculus-lsupporting

confidence: 89%

Untitled

Huang¹,

Xu²

2016

Turk. J. Fish. Aquat. Sci.

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“…Four biological replicates were used for each treatment. Sera collected from 5.0 ppm cadmium (as CdCl 2 $H 2 O)-exposed ayu [22] and 10 ppt (0.17 M NaCl) salinity-challenged ayu [23] were also used for Western blot analysis.…”

Section: A1-antitrypsinmentioning

confidence: 99%

Identification of α1-antitrypsin as a positive acute phase protein in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Lu¹,

Chen²,

Lü³

et al. 2012

Fish & Shellfish Immunology

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“…The trunk kidney proteome has been analyzed in ayu (Plecoglossus altivelis) [72]. Amino-terminal acetylation of renal proteins in sticklebacks: Both Nt-Ac and P-Hox are cotranslational modifications that occur when nascent polypeptide chains are folded into functional proteins in the endoplasmic reticulum [73,74].…”

Section: Discussionmentioning

confidence: 99%

Population-specific renal proteomes of marine and freshwater three-spined sticklebacks

Kültz

Paguio

et al. 2016

Journal of Proteomics

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Quantitative proteomics was used to reveal biochemical differences in kidneys of marine and freshwater three-spined sticklebacks. More than 1500 unambiguous proteins were identified, 106 of which are robustly co-translationally modified. Amino-terminal acetylation sites for 94 and proline hydroxylation sites for 12 proteins, including 4 protein disulfide isomerases having the consensus motif APWCGHCK, were determined. More than 1500 proteins were quantified by LC-MS/MS yielding 120 proteins with consistent population-specific abundance differences. Twenty-five of these were selected for validation by data-independent acquisition (DIA) and spectral library based MS2 quantitation. A dense biochemical network was revealed, which promotes the synthesis of the organic osmolytes betaine, sorbitol, trimethylamine oxid (TMAO), and urea. It contains 33 of 49 proteins that are elevated in marine compared to freshwater sticklebacks, including the most highly elevated proteins (dimethylaniline monooxygenase, alanine-glyoxylate aminotransferase, glycine N-methyltransferase). Freshwater stickleback kidneys contain elevated levels of proteolytic, cytoskeletal, extracellular matrix, and calcium signaling proteins. Proteins that are most elevated in freshwater sticklebacks are ES1 protein homolog, apoptosis-associated speck-like protein containing a CARD and caspase 1. Protein-abundance network analysis demonstrates significantly higher levels of synchronized abundance control in marine sticklebacks. The significance of these findings for biochemical diversification of renal function in marine and FW sticklebacks is discussed.

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The effects of environmental salinity on trunk kidney proteome of juvenile ayu (Plecoglossus altivelis)

Cited by 10 publications

References 26 publications

Untitled

Untitled

Identification of α1-antitrypsin as a positive acute phase protein in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Population-specific renal proteomes of marine and freshwater three-spined sticklebacks

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