The Effects of Endothelium-Specific CYP2J2 Overexpression on the Attenuation of Retinal Ganglion Cell Apoptosis in a Glaucoma Rat Model

Huang, Jingqiu; Zhao, Qinshuo; Duan, Qiming; Zhao, Yin; Zhang, Hong

doi:10.2139/ssrn.3353363

Cited by 2 publications

(7 citation statements)

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“…The protective effect of CYP2J2 on the vascular barrier is attributable to the compromised oxidative stress in ECs. In our previous study, CYP2J2 was confirmed to prevent endothelium from senescence 32 . Endothelial senescence gives rise to vascular permeability disorders 54,55 .…”

Section: Discussionmentioning

confidence: 87%

“…Rodents were anesthetized intraperitoneally with sodium pentobarbital. The retinal IR model was established as described previously 32 with modification. Under an operating microscope, a 30‐gauge metal needle containing a balanced salt solution was cannulated into the anterior chamber to maintain the intraocular pressure at 120 mmHg (measured using a microtonometer) for 45 min.…”

Section: Methodsmentioning

confidence: 99%

“…Retinal vascular preparations were performed as previously reported 32 with modification. Briefly, eyes were fixed in 4% paraformaldehyde for 2 d. Retinas were dissected and rinsed in distilled water overnight, followed by digestion at 37°C for 30 min with 5% pepsin.…”

Section: Methodsmentioning

confidence: 99%

“…The administration of 14,15‐EET suppressed neuronal apoptosis after cerebral ischemia–reperfusion (IR) injury 31 . Our previous study elucidated that CYP2J2 overexpression preserved retinal ganglion cells (RGCs) by protecting endothelium from senescence 32 . As an essential constituent of BRB, endothelium plays a crucial role in maintaining vascular integrity 33 .…”

Section: Introductionmentioning

confidence: 99%

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Endothelial CYP2J2 overexpression restores the BRB via METTL3‐mediated ANXA1 upregulation

et al. 2022

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Section: Discussionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endothelial CYP2J2 overexpression restores the BRB via METTL3‐mediated ANXA1 upregulation

et al. 2022

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“…Hence, Howell et al used bosentan in combination with a targeting complement system and showed a profound neuroprotection outcome in the DBA/2J mouse model (126). Huang et al found that in rat models, endothelial-specific overexpression of CYP2J2 protects the endothelium from senescence and prevents retinal ganglion cell loss by metabolizing arachidonic acid (127). This might indicate another potential future glaucoma treatment strategy.…”

Section: Potential Glaucoma Treatments Targeting the Endotheliummentioning

confidence: 99%

The characteristics of fundus microvascular alterations in the course of glaucoma: a narrative review

et al. 2022

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Background and Objective As the vascular theory has led many researchers to focus on vascular dysfunction in the pathogenesis of glaucoma, a better understanding of ocular microcirculation would be of great significance. The emergence of optical coherence tomography angiography (OCTA) has shed light on the various fundus microvascular changes that occur in glaucoma, thus providing ample evidence in the role of microvascular dysfunction in glaucoma. The aim of this review is to provide an overview of the retinal and choroidal microvascular alterations that occur in glaucoma and to address the role of microvascular alterations in the pathogenesis, diagnosis, prognosis, and treatment of glaucoma. Methods The literature regarding fundus microvascular alterations in glaucoma and after glaucoma treatment, including alterations of vascular perfusion and vascular reactivity, was broadly researched using PubMed and Web of Science databases. The endothelium involvements during the glaucoma course were also searched in the databases broadly. Key Content and Findings Previous OCTA studies show vessel density (VD) decreases in the retinal macular and peripapillary regions and choroidal microvascular dropout. Such microvascular alterations are correlated with structural and functional defects and have potential value for the early diagnosis and prognosis of glaucoma. Retinal microvascular autoregulation is also impaired in glaucomatous eyes. Furthermore, various studies have demonstrated the role of the vascular endothelium in glaucoma. Different topical medications and surgical interventions have been shown to have an impact on microvasculature in glaucoma, and animal experiments have indicated the endothelial system may be a new target in glaucoma treatment. Conclusions Ample evidence proved the involvement of retinal and choroidal microvascular structural and functional changes in the course of glaucoma. This review makes a novel contribution to the literature by summarizing the microvascular alterations in glaucoma eyes and the microvascular changes after topical or surgical treatments.

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The Effects of Endothelium-Specific CYP2J2 Overexpression on the Attenuation of Retinal Ganglion Cell Apoptosis in a Glaucoma Rat Model

Cited by 2 publications

References 0 publications

Endothelial CYP2J2 overexpression restores the BRB via METTL3‐mediated ANXA1 upregulation

Endothelial CYP2J2 overexpression restores the BRB via METTL3‐mediated ANXA1 upregulation

The characteristics of fundus microvascular alterations in the course of glaucoma: a narrative review

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