The effects of endosulfan on cytochrome P450 enzymes and glutathione S-transferases in zebrafish (Danio rerio) livers

Dong, Mingdong; Zhu, Lusheng; Shao, Bo; Zhu, Shaoyuan; Wang, Jun; Xie, Hui; Wang, Jinhua; Wang, Fenghua

doi:10.1016/j.ecoenv.2012.10.019

Cited by 57 publications

(30 citation statements)

References 58 publications

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“…This finding is similar to that of Dong et al [23], who showed significantly higher GST activities in endosulfan-treated groups than control groups. The roles of GSTs include conjugating endosulfan and other metabolites, and removing them from biological organisms, as has been shown in Planorbarius corneus and Aporrectodea caliginosa, which discard chlorpyrifos by inducing GST expression.…”

Section: Toxicity Of Endosulfan To Zebrafish Embryossupporting

confidence: 82%

“…Endosulfan has been known to induce the activity of cytochrome P450s, such as aminopyrine N-demethylase and erythromycin N-demethylase, in zebrafish livers. [23] The activity of cytochrome P450-dependent monooxygenases, such as 7-ethoxyresorufin-O-deacetylase, significantly increased in the livers of endosulfan-exposed Atlantic salmon (Salmo salar). [39] Therefore, the induction of CYP1A and 3A genes may be related to enhanced P450 monooxygenase activities in zebrafish.…”

Section: Toxicity Of Endosulfan To Zebrafish Embryosmentioning

confidence: 99%

“…Biochemical studies were conducted to determine its adverse effects during exposure to endosulfan because of inhibition on acetylcholinesterase expression and activity in Tigriopus japonicas, and possible induction of two detoxifying enzymes carboxylesterases and glutathione S-transferases (GST). [22][23][24] Additionally, endosulfan enhances genotoxicity and mutagenicity through oxidative stresses in Carassius carassius. [8] Therefore, the expression of 12 genes were measured using quantitative polymerase chain reaction (qPCR) techniques and the genes were involved in antioxidant activities and defense systems, and lipid and carbohydrate metabolism.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

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Acute toxicity and gene responses induced by endosulfan in zebrafish (Danio rerio) embryos

Moon

Jeon

Nam

et al. 2016

Chemical Speciation & Bioavailability

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Endosulfan has been listed as a persistent organic pollutant, and is frequently found in agricultural environments during monitoring processes owing to its heavy use and persistent characteristics. This study was conducted to understand the effects of endosulfan on the development of zebrafish (Danio rerio) embryos by exposing them to a specific range of endosulfan concentrations. Exposing zebrafish embryos to endosulfan for 96 h yielded no acute toxicity until the concentration reached 1500 μg L −1 , whereas malformed zebrafish larvae developed severely curved spines and shortened tails. About 50% of zebrafish larvae were malformed when exposed to 600 μg L −1 of endosulfan. Comparative gene expression using realtime quantitative polymerase chain reaction was assessed using endosulfan-exposed zebrafish embryos. CYP1A and CYP3A were significantly enhanced in response to endosulfan treatment. Two genes, acacb and fasn, encoding acetyl-CoA carboxylase b and fatty acid synthase proteins, respectively, were also up-regulated after treating zebrafish embryos with endosulfan. These genes are also involved in fatty acid biosynthesis. The genes encoding vitellogenin and Hsp70 increased in a concentration-dependent manner in embryos. Finally, biochemical studies showed that acetylcholinesterase activity was reduced, whereas glutathione S-transferase and carboxylesterase activities were enhanced in zebrafish embryos after endosulfan treatment. These biochemical and molecular biological differences might be used for tools to determine contamination of endosulfan in the aquatic environment.

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Section: Toxicity Of Endosulfan To Zebrafish Embryossupporting

confidence: 82%

Section: Toxicity Of Endosulfan To Zebrafish Embryosmentioning

confidence: 99%

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

See 1 more Smart Citation

Acute toxicity and gene responses induced by endosulfan in zebrafish (Danio rerio) embryos

Moon

Jeon

Nam

et al. 2016

Chemical Speciation & Bioavailability

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“…The decreased GST activity at 5 mg L À 1 treatment on day 21 may be because GSH, as a substrate, was largely consumed, and many intermediate metabolites produced in the process of detoxification could reduce GST activity or competitively inhibit GST substrates (e.g., CDNB) in vitro (Egaas et al, 1999). As for our results, Dong et al (2013a) reported that obviously increased GST activity compared with control groups was generally present in zebrafish.…”

Section: Oxidative Stress and Ros Levelsmentioning

confidence: 52%

Toxic effects of nitenpyram on antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers

Yan

Wang

Zhu

et al. 2015

Ecotoxicology and Environmental Safety

Self Cite

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“…Several authors have reported increased GST activity in different fish species exposed to pesticides, some of which are used in the study region. For example, Dong et al (2013) reported increased GST activity in Danio rerio larvae exposed to the organochlorine endosulfan and Oruc et al (2004) observed increased activity of this enzyme in two species of fish exposed to 2,4 D. Paulino et al (2012) observed an increase in GST activity in gills of the Neotropical fish Prochilodus lineatus after sub-chronic exposure to atrazine, a herbicide widely used to control undesirable organisms in corn crops (Mudiam et al, 2012). The present study also observed an increase in GST activity in gills of fish exposed to sites 3, 4, and 5, which have close proximity to corn fields.…”

Section: Discussionmentioning

confidence: 99%

Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas

et al. 2014

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Aquatic ecosystems in areas with intense agricultural activity are subject to pesticide contamination, which may compromise the health of the fish. In order to verify the quality of the water and the possible effects of pesticides on fish, a method that combines different biomarker responses into an index named "integrated biomarker response" (IBR) was applied using the biological alterations in the Neotropical fish Astyanax altiparanae. Fish were maintained in situ at five sites along a stream that runs in an agricultural area and in a stream within a forest fragment, considered a reference site. After seven days of exposure the following alterations were observed in fish confined at experimental sites: increased activity of glutathione-S-transferase (GST) and catalase (CAT) and increase in the content of reduced glutathione (GSH) in liver and gills, reduction of acetylcholinesterase (AChE) activity in the brain and muscle, increase in the occurrence of DNA strand breaks and in the frequency of micronuclei (MN) and nuclear abnormalities (ENA) in erythrocytes. The IBR highlighted three sites as the most affected, as the animals confined at these sites showed greater variations in biological responses. The biomarkers most important for the IBR results were GST, AChE, DNA breaks and ENA.Ecossistemas aquáticos inseridos em áreas com intensa atividade agrícola estão susceptíveis à contaminação por pesticidas, os quais podem comprometer a saúde dos peixes. A fim de verificar a qualidade da água e os possíveis efeitos de pesticidas sobre peixes, um método integrando o uso de diferentes biomarcadores, denominado índice integrado de respostas de biomarcadores (IBR), foi aplicado utilizando-se as alterações biológicas avaliadas em vários órgãos do peixe neotropical Astyanax altiparanae. Os peixes foram confinados in situ em cinco pontos ao longo de um ribeirão localizado em área de produção agrícola e em um córrego dentro de um fragmento florestal, considerado um local de referência. Após sete dias foram observadas as seguintes alterações nos peixes confinados nos pontos experimentais: aumento da atividade da glutationa-S-transferase (GST) e catalase (CAT) e aumento do conteúdo de glutationa-reduzida (GSH) em fígado e brânquias, redução da atividade da acetilcolinesterase (AChE) no músculo e cérebro, aumento de quebras no DNA e na frequência de micronúcleos (MN) e alterações nucleares (ENA) em eritrócitos. O IBR destacou três dos pontos estudados como os mais afetados pela contaminação agrícola, uma vez que foram observados nos animais confinados nestes locais maiores variações nas respostas biológicas. Os biomarcadores mais significativos para os resultados de IBR foram a GST, AChE, quebras no DNA e ENA.

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The effects of endosulfan on cytochrome P450 enzymes and glutathione S-transferases in zebrafish (Danio rerio) livers

Cited by 57 publications

References 58 publications

Acute toxicity and gene responses induced by endosulfan in zebrafish (Danio rerio) embryos

Acute toxicity and gene responses induced by endosulfan in zebrafish (Danio rerio) embryos

Toxic effects of nitenpyram on antioxidant enzyme system and DNA in zebrafish (Danio rerio) livers

Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas

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