The effects of emulsified polydimethylsiloxane FG‐10 on the oxygen transfer coefficient (k<sub>L</sub>a) and lipase production by<i>Staphylococcus warneri</i>EX17

Rech, Fernanda Roberta; Volpato, Giandra; Ayub, Marco Antônio Záchia

doi:10.1002/jctb.3710

Cited by 5 publications

(1 citation statement)

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“…Numerous carbon sources, such as Tween 80, triolein, yeast extract, olive oil, soybean oil, silk oil, sesame oil, coconut oil, and corn oil, have been used as substrates to produce lipases in cultures of bacteria (LI et al, 2005;BURKERT et al, 2004). However, few investigations have been carried out using glycerol as substrate (LIN et al, 2006;VOLPATO et al, 2008;RECH et al, 2012). Glycerol can be utilized as a carbon source in microbial bioconversions for the production of a range of chemicals that might be utilized either as end-products or as precursors for the production of other chemicals, since this biofuel will be produced on a large scale in the coming years, large amounts of glycerol will be discarded and can be used in bioprocesses.…”

Section: Introductionmentioning

confidence: 99%

Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

et al. 2020

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The industrial application of lipolytic enzymes has been studied mainly due to the ability of these enzymes in catalyze reactions of synthesis and their stability in various organic solvents. One possibility is the use of lipase the organic synthesis, taking advantage as the generation of waste and difficult recovery of sub bioproducts. In this work, we carried out a selection of eighty-four isolates of Bacillus amazonian for lipase production, of which 30 strains showed lipolytic activity. The study of the culture conditions was performed through a Plackett-Burman experimental design using the strain that presented the highest lipolytic activity in a culture medium using glycerol as substrate. The studied conditions were: concentration of soybean oil, olive oil, triton X-100, gum arabic, glycerol, and (NH4)2SO4, pH, temperature and concentration of inoculums. The best result obtained were 27 U/L in 48 h of cultivation by Bacillus circulans BL53. This work shows that the search and selection of microorganism with lipolytic activities can facilitate the discovery of new lipases, with potential use as by-product surplus.

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Section: Introductionmentioning

confidence: 99%

Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

et al. 2020

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Effects of metabolic pathway precursors and polydimethylsiloxane (PDMS) on poly-(gamma)-glutamic acid production by Bacillus subtilis BL53

Cesaro

Silva

Ayub

2014

Journal of Industrial Microbiology and Biotechnology

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The aims of this study were to evaluate the effects of the addition of metabolic precursors and polydimethylsiloxane (PDMS) as an oxygen carrier to cultures of Bacillus subtilis BL53 during the production of γ-PGA. Kinetics analyses of cultivations of different media showed that B. subtilis BL53 is an exogenous glutamic acid-dependent strain. When the metabolic pathway precursors of γ-PGA synthesis, L-glutamine and a-ketoglutaric acid, were added to the culture medium, production of the biopolymer was increased by 20 % considering the medium without these precursors. The addition of 10 % of the oxygen carrier PDMS to cultures caused a two-fold increase in the volumetric oxygen mass transfer coefficient (kLa), improving γ-PGA production and productivity. Finally, bioreactor cultures of B. subtilis BL53 adopting the combination of optimized medium E, added of glutamine, α-ketoglutaric acid, and PDMS, showed a productivity of 1 g L(-1) h(-1) of g-PGA after only 24 h of cultivation. Results of this study suggest that the use of metabolic pathway precursors glutamine and a-ketolgutaric acid, combined with the addition of PDMS as an oxygen carrier in bioreactors, can improve γ-PGA production and productivity by Bacillus strains .

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Improvement of AD Biosynthesis Response to Enhanced Oxygen Transfer by Oxygen Vectors in Mycobacterium neoaurum TCCC 11979

Shen

Gao

et al. 2017

Appl Biochem Biotechnol

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In steroid biotransformation, soybean oil can improve the productivity of steroids by increasing substrate solubility and strengthen the cell membrane permeability. However, little is known of its role as oxygen carrier and its mechanism of promoting the steroid biotransformation. In this work, soybean oil used as oxygen vector for the enhancement of androst-4-ene-3,17-dione (AD) production by Mycobacterium neoaurum TCCC 11979 (MNR) was investigated. Upon the addition of 16% (v/v) soybean oil, the volumetric oxygen transfer coefficient (K a) value increased by 44%, and the peak molar yield of AD (55.76%) was achieved. Analysis of intracellular cofactor levels showed high NAD, ATP level, and a low NADH/NAD ratio. Meanwhile, the two key enzymes of the tricarboxylic acid (TCA) cycle, namely, isocitrate dehydrogenase and α-ketoglutarate dehydrogenase, were upregulated after incubation with soybean oil. These enhancements induced by the increasing of oxygen supply showed positive effects on phytosterol (PS) bioconversion. Results could contribute to the understanding of effects of soybean oil as oxygen vector on steroid biotransformation and provided a convenient method for enhancing the efficiency of aerobic steroid biocatalysis.

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The effects of emulsified polydimethylsiloxane FG‐10 on the oxygen transfer coefficient (k_La) and lipase production byStaphylococcus warneriEX17

Cited by 5 publications

References 28 publications

Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

Effects of metabolic pathway precursors and polydimethylsiloxane (PDMS) on poly-(gamma)-glutamic acid production by Bacillus subtilis BL53

Improvement of AD Biosynthesis Response to Enhanced Oxygen Transfer by Oxygen Vectors in Mycobacterium neoaurum TCCC 11979

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The effects of emulsified polydimethylsiloxane FG‐10 on the oxygen transfer coefficient (kLa) and lipase production byStaphylococcus warneriEX17

Cited by 5 publications

References 28 publications

Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

Effects of metabolic pathway precursors and polydimethylsiloxane (PDMS) on poly-(gamma)-glutamic acid production by Bacillus subtilis BL53

Improvement of AD Biosynthesis Response to Enhanced Oxygen Transfer by Oxygen Vectors in Mycobacterium neoaurum TCCC 11979

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The effects of emulsified polydimethylsiloxane FG‐10 on the oxygen transfer coefficient (k_La) and lipase production byStaphylococcus warneriEX17