The Effects of Electroacupuncture on Th1/Th2 Cytokine mRNA Expression and Mitogen-Activated Protein Kinase Signaling Pathways in the Splenic T Cells of Traumatized Rats

Wang, Kun; Wu, Huaming; Wang, Guonian; Li, Mingming; Zhang, Zhaodi; Gu, Guangying

doi:10.1213/ane.0b013e3181b5a234

Cited by 48 publications

(46 citation statements)

References 27 publications

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“…In our previous study, surgical trauma was associated with decreased expression levels of IL-2 and IFN-γ, increased expression levels of IL-4 and IL-10, and immunosuppression in a surgical trauma rat model (27). In the present study, the expression levels of Th 17 -associated cytokines in patients with lung cancer were decreased following thoracotomy, which may have promoted an imbalance in numbers of Th 1 , Th 2 , Th 17 and T reg cells, and contributed to postoperative immunosuppression.…”

Section: Discussionmentioning

confidence: 86%

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th1, Th2, Th17 and Treg cells following thoracotomy of patients with lung cancer

Wang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. An imbalance in the various T lymphocytes, including T-helper (Th) 1 , Th 2 and Th 17 cells, and regulatory T (T reg ) cells, has been associated with immune dysfunction, and may occur following thoracotomy of patients with lung cancer. The use of transcutaneous acupoint electrical stimulation (TAES) has previously been demonstrated to exert immunoregulatory effects; therefore, the present study aimed to evaluate whether TAES was able to attenuate postoperative immune suppression in patients with lung cancer. Thoracic surgical patients with lung cancer (n=27) underwent TAES (frequency, 2/100 Hz; intensity, 4-12 mA) at the bilateral large intestine 4, pericardium 6, small intestine 3 and San Jiao 6 acupuncture points for 30 min, prior to incision, and at 20, 44, 68, 92 and 116 h following thoracotomy. The number of Th 1 , Th 2 , Th 17 and T reg cells, and the protein and mRNA expression levels of related cytokines were measured by flow cytometry, ELISA and polymerase chain reaction, respectively. The balance of Th 1 , Th 2 , Th 17 and T reg cells in the peripheral blood of patients with lung cancer was disrupted following thoracotomy. TAES administration increased the percentage of Th 1 and Th 17 cells, the protein expression levels of interleukin (IL)-2 and interferon-γ, the mRNA expression levels of T-bet and RAR-related orphan receptor-γt, and decreased the percentage of Th 2 cells, IL-10 protein expression levels, and GATA binding protein 3 mRNA expression levels. The results of the present study demonstrated that TAES was able to partially attenuate the postoperative immune depression of patients with lung cancer, by regulating the balance of Th 1 , Th 2 , Th 17 and T reg cells, and the expression levels of related cytokines and transcription factors; therefore, TAES may be considered to be a promising strategy for treating postoperative immune dysfunction in patients with lung cancer.

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Section: Discussionmentioning

confidence: 86%

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th1, Th2, Th17 and Treg cells following thoracotomy of patients with lung cancer

Wang

et al. 2015

Experimental and Therapeutic Medicine

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“…17 Cheng et al 18 noted that EA at ST36 acupuncture point might increase lymphocyte proliferation of surgically traumatised rats. 8 These results also suggested that acupuncture might improve immunosuppression after surgical trauma. A comparison with previous studies indicated that a similar effect of EA was also observed during craniotomy.…”

Section: Discussionmentioning

confidence: 88%

Electroacupuncture Alleviates Intraoperative Immunosuppression in Patients Undergoing Supratentorial Craniotomy

et al. 2013

Acupunct Med

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Background Clinical experience suggests that anaesthesia using a combination of acupuncture and drugs can reduce the dosage of anaesthetics required for craniotomy, decreasing both the disturbance in physiological functions during the operation and postoperative complications and improving the rate of recovery. The aim of the present study was to investigate the impact of electroacupuncture (EA) on the dynamic equilibrium of the immune system and immune cell populations during the pericraniotomy period. Methods A total of 56 patients undergoing craniotomy were randomised into three groups: control (C, n=18), EA (A, n=19) and sham acupuncture (S, n=19) groups. Blood samples were collected before anaesthesia (T0) and 30 min, 2 h and 4 h after induction of anaesthesia (T1, T2 and T3, respectively,) to measure the levels of tumour necrosis factor α (TNFα), interleukin (IL)-8, IL-10, IgM, IgA, IgG and full blood count. Results There was no significant difference between the measurements in groups A and S during craniotomy. The levels of IgM and IgA decreased significantly in group C compared with groups A and S at T2 and T3 time points. The levels of total T cells and suppressor T cells in group C decreased significantly compared with groups A and S at T1 and T2, and the level of natural killer cells in group C decreased significantly compared with groups A and S at T1. No significant differences between groups were found in the levels of TNFα, IgG, IL-10, IL-8, leucocytes, neutrophils, monocytes, Th cells or B cells. Conclusions EA appears to reduce immunosuppression of both the humoral and cellular components during surgery.

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“…Previous studies [12,13,14] and our experiment [15] demonstrated that the surgical trauma model of rats can imitate clinical features, and was used to illustrate the effects of surgical trauma and stress on the body function, and this model was relatively easy to perform. Surgical trauma and anesthesia were performed by two researchers who were not involved in data acquisition and analysis.…”

Section: Methodsmentioning

confidence: 91%

TNF-α- Mediated-p38-Dependent Signaling Pathway Contributes to Myocyte Apoptosis in Rats Subjected to Surgical Trauma

Wang

et al. 2015

Cell Physiol Biochem

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Background: The accumulation of cytokines in the plasma after trauma can induce myocyte apoptosis. We aimed to identify which cytokine(s) present in the plasma responsible for myocyte apoptosis, and delineated the signal transduction mechanism in rats subjected to surgical trauma. Methods: Rats were randomized into two groups: control and trauma groups, which was divided into five subgroups: posttraumatic 0, 3, 6, 12, and 24 h subgroups. Cardiomyocytes isolated from traumatized rats were incubated with one of the factors for 12 h (normal plasma; Cytomix; TNF-α; IL-1β; IFN-γ; trauma plasma; anti-TNF-α antibody; SB203580). Myocyte apoptosis, cytokine levels, and MAPKs activation, as the primary experimental outcomes, were measured by TUNEL, flow cytometry, ELISA and Western blot, respectively. Results: Myocyte apoptosis was induced by surgical trauma during the early stage after trauma. Accompanying this change, plasma TNF-α, IL-1β, and IFN-γ levels were elevated in traumatized rats. Incubation of traumatized cardiomyocytes with cytomix or TNF-α alone induced myocyte apoptosis, and increased the activation of p38 and ERK1/2. Myocyte apoptosis and p38 activation were elevated in traumatized cardiomyocytes with trauma plasma, and these increases were partly abolished by anti-TNF-α antibody or SB203580. Conclusion: Our study demonstrated that there exists the TNF-α-mediated-p38-dependent signaling pathway that contributed to posttraumatic myocyte apoptosis of rats undergoing surgical trauma.

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The Effects of Electroacupuncture on Th1/Th2 Cytokine mRNA Expression and Mitogen-Activated Protein Kinase Signaling Pathways in the Splenic T Cells of Traumatized Rats

Abstract: EA regulates a balance between Th1 and Th2 cytokines at protein and mRNA levels in splenic T cells, and, at least in part, involves the signaling pathways of ERK1/2, p38, NF-kappaB, and AP-1. The findings suggest that EA may improve immune suppression after surgical trauma.

Cited by 48 publications

References 27 publications

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th1, Th2, Th17 and Treg cells following thoracotomy of patients with lung cancer

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th1, Th2, Th17 and Treg cells following thoracotomy of patients with lung cancer

Electroacupuncture Alleviates Intraoperative Immunosuppression in Patients Undergoing Supratentorial Craniotomy

TNF-α- Mediated-p38-Dependent Signaling Pathway Contributes to Myocyte Apoptosis in Rats Subjected to Surgical Trauma

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