The effects of eggshell calcium (Biomin H^®) and its combinations with alfacalcidol (1α‐hydroxyvitamin D3) and menaquinone‐7 (vitamin K2) on ovariectomy‐induced bone loss in a rat model of osteoporosis

Abstract

The purpose of this study was to investigate the impact of eggshell calcium (Biomin H® dietary supplement) and its combinations with alfacalcidol (1α‐hydroxyvitamin D3) and menaquinone‐7 (vitamin K2) on ovariectomy‐induced bone loss in rats. Adult female rats (n = 48) were divided into 6 groups of 8 individuals each: sham‐operated rats (SHAM); ovariectomized (OVX) rats untreated; OVX rats treated with Biomin H® (BIO); OVX rats simultaneously receiving Biomin H®, vitamin D3 (BIO + D3); OVX rats simultaneously treated with Biomin H®, vitamin K2 (BIO + K2) and OVX rats treated with Biomin H®, vitamin D3, vitamin K2 (BIO + D3 + K2) during 8 weeks. Biochemical parameters, bone mineral density (BMD), bone mineral content (BMC) and femoral bone microstructure were determined. Plasma calcium and phosphate were increased in BIO + D3 and BIO + D3 + K2 groups as compared to OVX. Alkaline phosphatase was elevated in OVX, BIO versus SHAM, BIO + D3 + K2 groups. When compared to OVX group, decreased urine deoxypyridinoline was observed in all treated groups and femoral BMD, BMC were higher in BIO, BIO + D3, BIO + D3 + K2 groups. The BIO + K2 rats had similar densitometrical values than OVX individuals. Microcomputed tomography revealed increased trabecular relative bone volume (due to an increase in trabecular number) in BIO + D3, BIO + D3 + K2 as compared to OVX. The higher relative bone volume in BIO + D3, BIO + D3 + K2 groups was also accompanied by an increase in bone surface. In the cortical bone, an enhanced periosteal bone apposition was identified in BIO, BIO + D3, BIO + K2, BIO + D3 + K2 groups. The rats from BIO + D3 + K2 group had a higher area of primary osteon's vascular canals. In BIO + D3, BIO + K2, BIO + D3 + K2 groups, an increased area of secondary osteons was determined in comparison with OVX. Our results indicate the beneficial effect of triple application of Biomin H®, vitamin D3, vitamin K2, as well as simultaneous administration of Biomin H®, vitamin D3 on the inhibition of ovariectomy‐induced bone loss in a rat model of osteoporosis.

Bee Bread Can Alleviate Lipid Abnormalities and Impaired Bone Morphology in Obese Zucker Diabetic Rats

“…Examined parameters of the trabecular bone (BV/TV, Tb.N., Tb.Th., BMD, and BS) are shown in Table 2 . High resolution scans with 14.8 µm voxel size were obtained in both ROIs, including scanning parameters 70 kVp, 200 mA, 300 ms, and 0.5 mm aluminium filter [ 44 ]. Thin sections from cortical bone tissue (2D imaging) were prepared according to Martiniakova et al [ 45 ] and were evaluated by established classification systems [ 46 , 47 ].…”

Cornelian Cherry Pulp Has Beneficial Impact on Dyslipidemia and Reduced Bone Quality in Zucker Diabetic Fatty Rats

“…Microcomputed tomography (microCT, μCT 50, Scanco Medical, Brüttisellen, Switzerland) was used to designate selected quantitative 3D parameters of cortical bone (relative bone volume, relative bone volume with marrow cavity, BMD, cortical bone thickness and bone surface) and trabecular bone sections (relative bone volume, trabecular number, trabecular thickness, BMD and bone surface). The measurement parameters and scanning regions of interest were the same as those in the study by Omelka et al [ 28 ].…”

The Role of Macronutrients, Micronutrients and Flavonoid Polyphenols in the Prevention and Treatment of Osteoporosis

“…Similar results have been obtained in the study of Brennan et al [ 128 ] using calcium-rich marine multimineral complex which significantly preserved trabecular bone microarchitecture and slowed the onset of bone loss in comparison with calcium carbonate. Omelka et al [ 129 ] demonstrated the beneficial effects of co-administration of egg shell Ca with vitamins D3 and K2, as well as egg shell Ca with vitamin D3 only on the inhibition of bone loss in ovariectomized (OVX) rats. These two combinations significantly improved both biochemical and densitometric parameters consistent with osteoporosis.…”

“…Therefore, vitamin D supplementation, alone or in combination with Ca, appeared to be essential to enhance the positive effects of any specific therapy in such patients. In this context, simultaneous vitamin D and Ca intake in the treatment of osteoporosis is more effective than individual administrations [ 129 , 172 , 173 ].…”