The effects of drugs on wound healing: part 1

Karukonda, Sree R. K.; Flynn, Timothy C.; Boh, Erin E.; McBurney, Elizabeth I.; Russo, G; Millikan, Larry E.

doi:10.1046/j.1365-4362.2000.00948.x

Cited by 43 publications

(53 citation statements)

References 67 publications

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“…According to Baxter [4,9], contemporary research and consumption in physiotherapy is in particular focused on the stimulation of wound healing. Tissue repair and healing of injured skin are complex processes that involve a dynamic series of events including coagulation, inflammation, granulation tissue formation, wound contraction and tissue remodelling [10]. This complexity aggravates research within this cardinal indication.…”

Section: Introductionmentioning

confidence: 99%

Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation

Vinck

Cagnie

Cornelissen

et al. 2003

Lasers in Medical Science

268

144

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Background and Objective: As Light Emitting Diode (LED) devices are commercially introduced as an alternative for Low Level Laser (LLL) Therapy, the ability of LED in influencing wound healing processes at cellular level was examined. Study Design/Materials and Methods: Cultured fibroblasts were treated in a controlled, randomized manner, during three consecutive days, either with an infrared LLL or with a LED light source emitting several wavelengths (950 nm, 660 nm and 570 nm) and respective power outputs. Treatment duration varied in relation to varying surface energy densities (radiant exposures). Results: Statistical analysis revealed a higher rate of proliferation (p < 0.001) in all irradiated cultures in comparison with the controls. Green light yielded a significantly higher number of cells, than red (p < 0.001) and infrared LED light (p < 0.001) and than the cultures irradiated with the LLL (p < 0.001); the red probe provided a higher increase (p < 0.001) than the infrared LED probe and than the LLL source. Conclusion: LED and LLL irradiation resulted in an increased fibroblast proliferation in vitro. This study therefore postulates possible stimulatory effects on wound healing in vivo at the applied dosimetric parameters.

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Section: Introductionmentioning

confidence: 99%

Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation

Vinck

Cagnie

Cornelissen

et al. 2003

Lasers in Medical Science

268

144

View full text Add to dashboard Cite

show abstract

“…The positive effects were observed only in injured cells; no benefit was observed in healthy cells. Tissue repair and healing of injured skin are complex processes that involve a dynamic series of events including coagulation, inflammation, granulation tissue formation, wound contraction, and tissue remodeling [16]. The ideal wavelengths were reported to be between 600 and 900 nm with the best results obtained at specific ranges: 610-625, 660-690, 750-770, and 815-860 nm.…”

Section: Introductionmentioning

confidence: 99%

Effect of 660 nm Light-Emitting Diode on the Wound Healing in Fibroblast-Like Cell Lines

Kim

Cho

Kook

et al. 2015

International Journal of Photoenergy

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Light in the red to near-infrared (NIR) range (630-1000 nm), which is generated using low energy laser or light-emitting diode (LED) arrays, was reported to have a range of beneficial biological effects in many injury models. NIR via a LED is a well-accepted therapeutic tool for the treatment of infected, ischemic, and hypoxic wounds as well as other soft tissue injuries in humans and animals. This study examined the effects of exposure to 660 nm red LED light at intensities of 2.5, 5.5, and 8.5 mW/cm 2 for 5, 10, and 20 min on wound healing and proliferation in fibroblast-like cells, such as L929 mouse fibroblasts and human gingival fibroblasts (HGF-1). A photo illumination-cell culture system was designed to evaluate the cell proliferation and wound healing of fibroblast-like cells exposed to 600 nm LED light. The cell proliferation was evaluated by MTT assay, and a scratched wound assay was performed to assess the rate of migrating cells and the healing effect. Exposure to the 660 nm red LED resulted in an increase in cell proliferation and migration compared to the control, indicating its potential use as a phototherapeutic agent.

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“…7 However, it is mainly responsible for collagen deposition. 8 Fibroblast availability can be measured in vitro by observing its function in making a contraction on a media. 9 Fibroblast Populated Collagen Lattice Contraction (FPCL) media is a model that can be used to measure fibroblast contraction.…”

Section: Introductionmentioning

confidence: 99%

The effect povidone-iodine on the wound healing process: A study on fibroblast populated collagen lattice (FPCL) model

Danarti

Suswardana²,

Budiyanto

et al. 2014

JMedScie

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Povidone-iodine (PI) 10% solution is an effective antiseptic. However, it appears to be toxic to the cells involved in wound healing. The aim of this study is to evaluate the toxicity of PI on cultured human fibroblast using fibroblast populated collagen lattice (FPCL) model. The cultured human fibroblast was divided into 6 groups i.e. 5 groups were exposed by PI 1, 0.1, 0.01, 0.001 and 0.0001%, and 1 group was exposed by phosphate-buffered-saline (PBS). Twenty-four hours later, the media was washed using PBS. The size of the FPCL media on each group was observed over time by serial photographs, which then were measured by Image-J computer program. Exposure of 0.1, 0.01, 0.001 and 0.0001 PI caused an obvious reduction of fibroblast's contraction capability on FPCL media, which described temporary fibroblast injury, that showing a concentration-dependent recovery phenomenon after 48 th hour. Furthermore, 1% PI exposure leads to a permanent fibroblast injury. In conclusion, PI exposure in concentration more than 0.1% has a permanent toxic effect on fibroblast that clearly observed using a simple FPCL model. ABSTRAKLarutan povidon iodida 10% adalah suatu antiseptik yang efektif. Namun demikian, larutan ini kemungkinan toksik terhadap sel yang terlibat dalam proses penyembuhan luka. Penelitian ini bertujuan untuk mengkaji toksisitas PI pada kultur fibroblas manusia menggunakan model fibroblast populated collagen lattice (FPCL). Kulture fibroblas manusia dibagi menjadi 6 kelompok yaitu 5 kelompok dipapar dengan larutan 1; 0,1; 0,01; 0,001; dan 0,0001 % PI dan 1 kelompok dipapar dengan salin dapar fosfat (PBS). Dua puluh empat jam kemudian, media dicuci dengan larutan PBS. Ukuran media FPCL pada masing-masing kelompok diamati dari waktu ke waktu dengan fotograf secara serial untuk kemudian diukur dengan program komputer Image-J. Paparan PI 0,1; 0,01; 0,001 dan 0,0001 menyebabkan penurunan nyata kemampuan kontraksi fibroblas pada media FPCL yang menggambarkan kerusakan fibroblas sementara. Kerusakan ini menunjukkan fenomena perbaikan yang tergantung konsentrasi setelah jam ke 48. Selanjutnya, paparan PI 1% menyebabkan kerusakan fibroblas secara permanen. Dapat disimpulkan, paparan PI pada konsentrasi lebih dari 0,1% menyebabkan efek toksik permanen pada fibroblas yang jelas teramati dengan model FPCL sederhana.

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The effects of drugs on wound healing: part 1

Cited by 43 publications

References 67 publications

Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation

Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation

Effect of 660 nm Light-Emitting Diode on the Wound Healing in Fibroblast-Like Cell Lines

The effect povidone-iodine on the wound healing process: A study on fibroblast populated collagen lattice (FPCL) model

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