The effects of drugs and denervation on removal and accumulation of noradrenaline in the perfused hind-limb of the dog

Osswald, Walter; Branco, D.

doi:10.1007/bf00501158

Cited by 20 publications

(8 citation statements)

References 21 publications

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“…Recent observations show a delay of only a fraction of a second between the commencement of electrical stimulation and the beginning of contractile response in this preparation [Brandao, personal commun.]. On the other hand, such intimate contacts may help explain why transmitter inactivation in the vein is mainly effected through neuronal re-uptake, as evidenced by the large change in sen sitivity with cocaine [Brandao and G uimaraes, 1974], This neuronal up take was shown to contribute markedly to inactivation of not only endogen ous but also the exogenous cathecolamines G uima raes el al., 1971;Osswald and Branco, 1973]. This contrasts with what happens in the rabbit aortic strip [Kalsner and Nickerson, 1969], a sparsely innervated structure with an adventitio-medial adrenergic plexus.…”

Section: Discussioncontrasting

confidence: 46%

Fine Structural and Autoradiographic Study of the Adrenergic Innervation of the Dog Lateral Saphenous Vein

Coimbra¹,

Ribeiro‐Silva²,

Osswald³

1974

J Vasc Res

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The tunica media of the dog lateral saphenous vein contains 8–12 layers of predominantly circular smooth muscle fibers. Numerous nerve profiles exhibit bright green fluorescence after formaldehyde gas treatment. These profiles occur amongst the muscle cells throughout the whole thickness of the media. Electron microscopy shows such profiles to be surrounded by Schwann cell processes enveloping axons which contain large (43%) and small (57%) dense-core vesicles. Axons are separated from muscle cells by 100–300 nm; in some cases intimate contacts, ≈20 nm wide, were seen between an axon and a muscle cell projection. After incubation of the vein with exogenous radioactive noradrenaline, nerves showed a great accumulation of radioactive material. Extraneuronal tissues (muscle and connective tissue) exhibit a much lower grain density (≈15 times less), which is, however, far above background levels. The dense innervation of the vessel wall and the intimate neuromuscular relationship are considered to form the morphological basis for the speed of nerve-evoked contractile responses in this preparation, as well as for the great extent of transmitter inactivation through neuronal reuptake.

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Section: Discussioncontrasting

confidence: 46%

Fine Structural and Autoradiographic Study of the Adrenergic Innervation of the Dog Lateral Saphenous Vein

Coimbra¹,

Ribeiro‐Silva²,

Osswald³

1974

J Vasc Res

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“…The fact that the IRR-amplitude curves were similarly displaced by by known inhibitors of neuronal uptake, DMI (Titus & Spiegel, 1962;Callingham, 1967) and cocaine (Iversen, 1963;Osswald & Branco, 1973), suggests that the 'initial rate of recovery' is related to the rate of elimination of released transmitter. Furthermore, the effect of phenoxybenzamine, which depressed the response to all frequencies of stimulation without altering the relationship between response amplitude and IRR, suggests that this relationship is a consequence of some fundamental property of the tissue, such as the shape of the agonist concentration-response curve.…”

Section: Phenoxybenzaminementioning

confidence: 99%

RELAXATION OF SMOOTH MUSCLE FOLLOWING CONTRACTION ELICITED BY SYMPATHETIC NERVE STIMULATION in vivo

Eccles

MacLean

1977

British J Pharmacology

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A method for studying in vivo the process of neuroeffector transmission in the nictitating membrane and nasal blood vessels of the cat is described. 2 Administration of desmethylimipramine or cocaine caused increases in both the amplitude and duration of the nasal and membrane responses which may be explained by inhibition of neuronal uptake of noradrenaline. 3 Phenoxybenzamine depressed the responses to nerve stimulation, but had little effect on the relationship between response amplitude and rate of recovery. 4 The relationship between response amplitude and rate of recovery is discussed and related to the sigmoid shape of a log concentration-response curve.

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“…at least in part, to an increased extraneuronal deamination, as the results obtained with co caine show. This increased formation of DOMA probably represents a vicarious enzy matic pathway, established by the progres sive degeneration of adrenergic terminals; vi carious enzymatic pathways have been pro posed for blood vessels, under other experi mental conditions, by Brandào and Guimaràes (1974), Osswald and Branco (1973) and Levin (1975).…”

Section: Mesenteric Arteriesmentioning

confidence: 99%

Uptake and Metabolism of Noradrenaline by Blood Vessels of Perinephritic Hypertensive Dogs

Garrett¹,

Castro-Tavares²,

Branco³

1981

J Vasc Res

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Tissue noradrenaline content as well as uptake and metabolism of tritiated exogenous noradrenaline were studied comparatively, in vitro in mesenteric arteries, and in saphenous veins of normotensive and perinephritic hypertensive dogs. The influence of cocaine, iproniazid and 3’-4’-dihydroxy-2-methyµ-propiophenone (U-0521) on these variables wasalso investigated. The concentration of (–)-7-³H-noradrenaline used was 1.084 µM. No changes were observed in noradrenaline content, uptake and metabolism in saphenous vein strips obtained from normotensive or hypertensive animals. However, in mesenteric artery strips obtained from hypertensive dogs, a marked reduction in endogenous noradrenaline content was observed as well as a reduction in noradrenaline accumulation (20 weeks after surgery). The deamination pattern was also modified in these strips: the formation of DOPEGwas markedly diminished and the formation of DOMA was increased. These results agree well with the degeneration of the sympathetic innervation of the mesenteric arteries of hypertensive dogs described by Azevedo et al. (1981).

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The effects of drugs and denervation on removal and accumulation of noradrenaline in the perfused hind-limb of the dog

Cited by 20 publications

References 21 publications

Fine Structural and Autoradiographic Study of the Adrenergic Innervation of the Dog Lateral Saphenous Vein

Fine Structural and Autoradiographic Study of the Adrenergic Innervation of the Dog Lateral Saphenous Vein

RELAXATION OF SMOOTH MUSCLE FOLLOWING CONTRACTION ELICITED BY SYMPATHETIC NERVE STIMULATION in vivo

Uptake and Metabolism of Noradrenaline by Blood Vessels of Perinephritic Hypertensive Dogs

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