The Effects of Drought and Flood Stress on Pollen Quality and Quantity in Clivia miniata (Lindl.) Bosse (Amaryllidaceae)

Yamburov, M. S.; Astafurova, T.P.; Zhuk, Konstantin Vitalievich; Romanova, S B; Smolina, Valentina Mikhailovna

doi:10.13005/bpj/526

Cited by 8 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various environmental factors such as geographical conditions and change of climate can influence on the size of pollen grains (Dajoz et al, 1991;Delph et al, 1997;Ejsmond et al, 2011Ejsmond et al, , 2015Yamburov et al, 2014). Ejsmond et al (2011) studied influence of temperature increased on the pollen morphology, pollen size and pollen shape.…”

Section: Resultsmentioning

confidence: 99%

“…Plants are known to produce fewer pollen grains but larger sizes to avoid desiccation stress during the flowering period which studied some authors (Ejsmond et al, 2011;Mehmood et al, 2023). Yamburov et al (2014) studied effects of drought and flood stress on pollen quality and quantity in Clivia miniata including pollen size, viability, germination, and number of pollen grains per anther while evaluating how stress influences these features. The study provides evidence that microsporogenesis of Clivia miniata is more sensitive to flooding than to drought.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Pollen Morphology of Some Species of the Genus Amelanchier Medik.

Horčinová Sedláčková

2023

Agrobiodivers Improv Nutr health life Qual

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Pollen Morphology of Some Species of the Genus Amelanchier Medik.

Horčinová Sedláčková

2023

Agrobiodivers Improv Nutr health life Qual

View full text Add to dashboard Cite

show abstract

“…7 ). Different levels of pollen sterility also characterized M. domestica “McIntosh” diploids and mixoploids and C. miniata subjected to drought and flood conditions (Yamburov et al 2014 ; Podwyszyńska et al 2016 ). Unfortunately, the genotype of the old apple tree that is conserved at the botanical garden of Turin could not be traced.…”

Section: Discussionmentioning

confidence: 99%

Quantitative methods in microscopy to assess pollen viability in different plant taxa

et al. 2020

View full text Add to dashboard Cite

High-quality pollen is a prerequisite for plant reproductive success. Pollen viability and sterility can be routinely assessed using common stains and manual microscope examination, but with low overall statistical power. Current automated methods are primarily directed towards the analysis of pollen sterility, and high throughput solutions for both pollen viability and sterility evaluation are needed that will be consistent with emerging biotechnological strategies for crop improvement. Our goal is to refine established labelling procedures for pollen, based on the combination of fluorescein (FDA) and propidium iodide (PI), and to develop automated solutions for accurately assessing pollen grain images and classifying them for quality. We used open-source software programs (CellProfiler, CellProfiler Analyst, Fiji and R) for analysis of images collected from 10 pollen taxa labelled using FDA/PI. After correcting for image background noise, pollen grain images were examined for quality employing thresholding and segmentation. Supervised and unsupervised classification of per-object features was employed for the identification of viable, dead and sterile pollen. The combination of FDA and PI dyes was able to differentiate between viable, dead and sterile pollen in all the analysed taxa. Automated image analysis and classification significantly increased the statistical power of the pollen viability assay, identifying more than 75,000 pollen grains with high accuracy (R2 = 0.99) when compared to classical manual counting. Overall, we provide a comprehensive set of methodologies as baseline for the automated assessment of pollen viability using fluorescence microscopy, which can be combined with manual and mechanized imaging systems in fundamental and applied research on plant biology. We also supply the complete set of pollen images (the FDA/PI pollen dataset) to the scientific community for future research.

show abstract

“…Although there are no specific studies regarding the effect of these phenomena on pollen of RS species, research on pollen from orthodox species suggests that microsporogenesis could be more sensitive to flooding than to drought, affecting its quantity, viability, germination and size (Yamburov et al, 2014). The effect on size is of particular importance since some studies suggest that the size of the pollen grain is a variable that could affect the tolerance to cryopreservation (Ren et al, 2019).…”

Section: Effects Of Increased Temperatures On Pollenmentioning

confidence: 99%

The potential impacts of climate change on ex situ conservation options for recalcitrant-seeded species

Fernández

León‐Lobos

Contreras

et al. 2023

Front. For. Glob. Change

View full text Add to dashboard Cite

Recalcitrant seeds are characterized by desiccation and freezing sensitivity, and short storage longevity. These physiological attributes obviate their ex situ conservation in conventional seed banks, where seeds are stored dry at sub-zero temperatures (typically, 15% relative humidity and –20°C) for extended periods of time. Propagation of plants for field collections (e.g., botanical gardens, nurseries, and arboretums) is a valuable ex situ conservation option. However, these collections are relatively costly, require high maintenance, preserve limited genetic diversity and/or are directly exposed to biotic (e.g., pests) and abiotic (e.g., climatic) threats. Therefore, recalcitrant-seeded (RS) species are dependent on cryopreservation for their safe and long-term ex situ conservation. Different explant sources such as whole seeds, zygotic embryos, dormant buds, shoot tips, and pollen, can be used for plant propagation of RS species in field collections as well as for their cryopreservation. The success of the propagation or the cryopreservation of these explants often depends on their developmental status, vigor, and/or tolerance to desiccation and chilling/freezing. These attributes are modulated by the environment where the donor plant grows and we hypothesize that climate change, by affecting these biological attributes, would impact the success of explant propagation and cryopreservation. To support this hypothesis, we have reviewed how temperature changes and drought, the two main climate change scenarios, affect the main biological attributes that are directly involved in the success of ex situ conservation of tropical and temperate RS species. In general, increases in temperature and drought will negatively affect plant development in field collections and the quality of the explants used in cryopreservation. Consequently, field collections of RS species may need to be moved to more suitable places (e.g., higher latitudes/altitudes). Additionally, we may find a reduction in the success of cryopreservation of RS species germplasm directly harvested from field collections. However, we cannot always generalize these effects for all species since they often depend on the origin of the species (e.g., tropical and temperate species tend to respond to climate change differently), the genotype, the adaptive genetic potential of each population, and the severity of the environmental change. On the other hand, the increase in temperatures and water stress in donor plants at high-latitude areas and also some tropical environments may favor the production of seeds and seedlings better adapted to drying, and hence, increase the success of plant propagation and zygotic embryo cryopreservation.

show abstract

The Effects of Drought and Flood Stress on Pollen Quality and Quantity in Clivia miniata (Lindl.) Bosse (Amaryllidaceae)

Cited by 8 publications

References 19 publications

Pollen Morphology of Some Species of the Genus Amelanchier Medik.

Pollen Morphology of Some Species of the Genus Amelanchier Medik.

Quantitative methods in microscopy to assess pollen viability in different plant taxa

The potential impacts of climate change on ex situ conservation options for recalcitrant-seeded species

Contact Info

Product

Resources

About