The effects of different silage additives on rumen protozoan number and volatile fatty acid concentration in sheep fed corn silage

The study was conducted to evaluate the effects of adding a fibrolytic enzyme in combination with bacterial inoculants on the fermentation, chemical composition and aerobic stability of ensiled potato hash (PH). Potato hash silage (PHS) was produced by mixing 800 g PH/kg and 200g wheat bran (WB)/kg. The mixture was ensiled with either no additive or enzyme Celluclast (low or high dose) or bacterial inoculants (Emsilage and Silosolve). These treatment combinations were produced: i) no additive (control); ii) Celluclast low dose (CLD); iii) Celluclast high dose (CHD); iv) Emsilage (EMS); v) CLD + EMS; vi) CHD + EMS; vii) Silosolve (SLS); viii) CLD + SLS; and ix) CHD + SLS. These treatments were ensiled in 81 x 1 L anaerobic jars for 90 days with nine replicates per treatment. Three samples per treatment were collected before ensiling and after 90 days' ensiling, were analysed for fermentation characteristics and chemical composition. In addition, samples of day 90 were subjected to an aerobic stability test, where they were exposed for five days. Enzyme addition reduced fibre, thus making more sugar available for fermentation. The combination of CHD and EMS reduced silage pH, thus preserving the silage compared with other treatment combinations. Enzyme addition (used at low and high dose), and bacterial inoculants improved fermentation. Enzyme addition improved the chemical composition, but impaired the aerobic stability of PHS. Further work to test these findings on animal performance is warranted.

Section: Resultssupporting

confidence: 90%

Effects of a fibrolytic enzyme and bacterial inoculants on the fermentation, chemical composition and aerobic stability of ensiled potato hash

Mutavhatsindi¹,

Nkosi²,

Baloyi³

et al. 2018

“…Monteils et al (2012) reported that the rumen protozoa count of bulls fed diets based on CS was 701.000 in 1 ml rumen fluid. The results of the study for rumen protozoa count were consistent with those of Dönmez et al (2003) and Monteils et al (2012). *Inulin added at 0 (CSC, PRC, VOC), 100 (CS100, PR100, VO100), 200 (CS200, PR200, VO200), and 300 (CS300, PR300, VO300) mg/litre of total culture fluid; CS: corn silage; VO: vetch/oat hay; PR: perennial ryegrass Several factors (plant type, climate, season, soil type and fertility, etc.)…”

Section: Resultssupporting

confidence: 86%

“…was the dominant species in the rumen fluid taken separately from each feedstuff. Dönmez et al (2003) reported that the counts of rumen protozoa in ruminants fed various diets ranged from 380.000 to 1.160.000 per ml rumen fluid. Monteils et al (2012) reported that the rumen protozoa count of bulls fed diets based on CS was 701.000 in 1 ml rumen fluid.…”

Section: Resultsmentioning

confidence: 99%

Effects of various inulin levels on in vitro digestibility of corn silage, perennial ryegrass (Lolium perenne L.) and common vetch (Vicia sativa L.)/oat (Avena sativa L.) hay

Salman¹,

Çetinkaya²,

Selçuk³

et al. 2017

Effects of various inulin levels on in vitro ______________________________________________________________________________________ AbstractThe aim of this study was to investigate the effects of various inulin levels on in vitro true dry matter digestibility (IVTD DM ) and in vitro neutral detergent fibre digestibility (IVTD NDF ) of corn silage (CS), perennial ryegrass (PR), and common vetch/oat hay (VO). Inulin was added to the fermenter at concentrations of 0 (CSC, PRC, VOC), 100 (CS100, PR100, VO100), 200 (CS200, PR200, VO200), and 300 (CS300, PR300, VO300) mg/litre of total culture fluid using an in vitro Daisy II incubator. Each fermenter contained 1600 ml buffer solution and 400 ml rumen fluid.

“…Similar effects on increased VFA production in sheep fed molasses-treated corn silage have been shown (Donmez et al, 2003). An increased gas production has been related to improved silage quality (Hetta et al, 2007), which would also determine the microbial access to fermentable carbohydrates in the rumen.…”

Section: Discussionmentioning

confidence: 54%

The effects of wilting, molasses and inoculants on the fermentation quality and nutritive value of lucerne silage

Hashemzadeh¹,

Khorvash²,

Ghorbani³

et al. 2011

The objective was to determine the effects of wilting, molasses and inoculants on the biochemistry and in vitro and in situ digestion of lucerne silage. Lucerne containing 200 g/kg of dry matter (DM) was ensiled as fresh or wilted (370 g/kg DM). Molasses, at application rates of 0, 50 and 100 g molasses/kg DM, was added to the chopped lucerne. Within each molasses treatment, the lucerne was applied with distilled water or with the inoculants, Ecosyl or Lalsil. Wilting lucerne increased the silage DM and water soluble carbohydrates and decreased neutral detergent fibre, acid detergent fibre, ammonia and the acetate content of the silage. Adding 50 and 100 g molasses/kg to wilted lucerne and 100 g molasses/kg to fresh lucerne lowered the silage pH. Adding molasses to wilted lucerne increased the acetate content of the silage. In wilted but not in fresh lucerne both inoculants decreased the concentration of unavailable N in the silage. In wilted lucerne, Lalsil, but not Ecosyl, reduced the silage acetate level and in fresh lucerne both inoculants reduced the acetate level. Lalsil was more effective in wilted silages in improving the fermentation quality than Ecosyl. Both inoculants enhanced the 24-h rumen degradation of silage DM, with Lalsil being effective in wilted lucerne and Ecosyl in fresh lucerne. Molasses, at 100 g/kg, improved the in vitro silage organic matter digestion at 6, 8, 36 and 48 h post-incubation. It was concluded that inoculating lucerne crops with Lalsil improved the fermentation quality as well as nutritive value and lowered proteolysis. These effects were more pronounced in silage with a high DM content.