The effects of different potato dextrose agar media on secondary metabolite production in Fusarium

Westphal, Klaus Ringsborg; Heidelbach, Søren; Zeuner, Emil Juel; Riisgaard-Jensen, Marie; Nielsen, Morten Eneberg; Vestergaard, Sofie Zacho; Bekker, Nicolai Sundgaard; Skovmark, Jesper; Olesen, Christian Kjær; Thomsen, Katrine Hartmann; Niebling, Sara Kramer; Sørensen, Jens Laurids; Søndergaard, Teis Esben

doi:10.1016/j.ijfoodmicro.2021.109171

Cited by 16 publications

(11 citation statements)

References 30 publications

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“…Similarly, Kokkonen et al [ 54 ] observed the distinctive influence of the culture medium on mycotoxin production by some Penicillium species. Westphal et al [ 55 ] described significant differences in the metabolite profile of four Fusarium species, depending on the manufacturer of the Potato Dextrose Agar (PDA), a commonly used medium for fungi cultivation.…”

Section: Resultsmentioning

confidence: 99%

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Steglińska

Sulyok

Janas

et al. 2023

IJERPH

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The potato is a crop of global importance for the food industry. This is why effective protection against pathogens is so important. Fungi as potato pathogens are responsible for plant diseases and a significant reduction in yields, as well as for the formation of mycotoxins. This study focuses on the effect of three natural biocides, yeast Metschnikowia pulcherrima, lactic acid bacteria Lactiplantibacillus plantarum, and aqueous garlic extract, on the improvement of the physiology of planted potato tubers and the reduction in mycotoxin formation. The secondary metabolites produced by the fungal pathogens of genera Fusarium, Alternaria, Colletotrichum, Rhizoctonia, and Phoma in the presence of these biocontrol agents were compared to profiles obtained from contaminated potatoes. Analysis of liquid chromatography coupled with tandem mass spectrometry data showed the presence of 68 secondary metabolites, including the mycotoxins: alternariol, alternariol methyl ether, altertoxin-I, aurofusarin, beauvericin, diacetoxyscirpenol, enniatin B, and sterigmatocystin. The studies showed that the applied biocontrol agents had a positive effect on the physiological parameters of potatoes (including root growth, stem growth, gas exchange, and chlorophyll content index) and on the reduction in the production of mycotoxins and other secondary metabolites by Fusarium, Alternaria, and Phoma.

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Section: Resultsmentioning

confidence: 99%

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Steglińska

Sulyok

Janas

et al. 2023

IJERPH

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“…Growth characters of F. oxysporum f. sp. gerberae studied on different solid media indicated that the growth was maximum on Oat meal agar followed by Richards's agar, Czapek's Dox agar, and Potato Dextrose agar supported maximum growth of fungal colony (Rajirani et al, 2000;Rajirani 2001;Chittem and Kulkarni, 2008;Mezzomo et al, 2018;Westphal et al, 2021). These studies revealed that among the solid substrates, leafy substrates, bran and oil seed cakes, and the liquid substrates tested mature coconut water supported maximum biomass and macrconidia.…”

Section: Effect Of Different Mediamentioning

confidence: 97%

Fusarium pallidoroseum: A potential entomopathogenic agent for the biological management of Aphis gossypii

Srivastava

Shukla

2021

JANS

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With rising need of switching over to sustainable agricultural practices, utilization of entomopathogenic fungi (EPF) as biocontrol agents, provides better substitute against chemical pesticides- having several side-effects. Therefore, an attempt have been made to explore the potential EPF fungi that could be incorporated into IPM practices for control of Helicoverpa armigera Hubner and Aphis gossypii Glover. Regarding this, an entomopathogenic fungus, Fusarium pallidoroseum (Cooke) Sacc, was isolated from natural population of H. armigera infesting chickpea (Cicer arietinum L.) and explored efficacy under in-vitro & field conditions. The findings of present investigation shows efficacy of F. pallidoroseum as potential biocontrol agent against okra aphid (A. gossypii), as it inflicted initial mortality of 43.33% nymphs on 2nd day and lead to complete annihilation (93.33%) of nymph population on 8th day of spore suspension application at 1x1010 spores/mL concentration. The observations against adult okra aphid clearly demonstrated that spraying of 1 x 1010 spores/mL of F. pallidoroseum resulted 66.67% mortality after 8th days of spraying. Increased mortality was recorded with increase in spore suspension concentrations. The LC50 & LC90 value for F. pallidoroseum against nymphs of A. gossypii was recorded 3.79 x 105 and 2.74 x 108, respectively. The findings were used to develop formulations (1 × 104 to 1 × 1010 spore suspension/mL conc), and tested at field-level. The results showed that formulation at 1 x 1010 spores/mL conc was most effective against A. gossypii, recorded 93.33% mortality of nymphs & 66.67% mortality of adults; could be used under IPM practices.

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“…Pada media PDA, kapang dapat tumbuh dengan baik karena PDA ini mempunyai beberapa nutrisi essensial untuk pertumbuhan kapang (Westphal et al, 2021). Menurut Gabriel et al (2018), kapang tumbuh dengan baik dan menyebar ke setiap sudut petri untuk memaksimalkan pencarian nutrisi pada media.…”

Section: Hasil Dan Pembahasanunclassified

Degradasi Karbohidrat pada Pakan Udang oleh Isolat Kapang Endofit Mangrove

et al. 2023

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Feed quality is a very important factor in shrimp farming because it will affect shrimp growth, water quality and even the emergence of pathogenic bacteria. High quality feed can be well digested by shrimp so that it can improve the growth and do not leave plenty of residue. Mold is capable of producing cellulase which can be used to improve feed quality by shortening carbohydrate chains. This paper will discuss the application of endophytic molds to simplify cellulose in shrimp feed. Mold isolates were collected from mangrove ecosystems in North Sulawesi. The media used for culturing mold are potato dextrose agar (PDA) and potato dextrose broth (PDB). Fermentation test was conducted using the fungal isolates; Hypocreales sp. and Diaporthe stewartii and consortia and controls. Fermentation results were checked by TLC and Fehling's test to determine the composition of the compounds and the presence of reducing sugar either mono or di-saccharide. Fermented shrimp feed shows differences in texture and color. Fermented feed extract with Hypocreales sp. 2.69 g (3.36%), Diaporthe stewartii 4.9 g (6.13%), and consortium 3.75 g (4.69%). TLC results neither under UV light nor visualization of vanillin sulfate did not show any differences of compounds in the control and the fermented feed. The results of the Fehling test showed that the mold was able to degrade cellulose that can be utilized to increase the shirmp feed quality. Kualitas pakan merupakan faktor yang sangat berpengaruh dalam budidaya udang karena akan mempengaruhi pertyumbuhan udang, kualitas air bahkan timbulnya bakteri pathogen. Pakan yang baik mudah dicerna oleh udang sehingga dapat meningkatkan pertumbuhan secara maksimal dan tidak terlalu banyak meninggalkan residu. Kapang mampu memproduksi selulase yang dapat digunakan untuk meningkatkan kualitas pakan dengan memperpendek rantai karbohidrat. Tulisan ini akan membahas aplikasi kapang endofit untuk menyederhanakan selulosa pada pakan udang. Isolat kapang dikoleksi dari ekosistem mangrove di Sulaweri Utara. Media yang digunakan untuk mengkultur kapang adalah potato dextrose agar (PDA) dan potato dextrose broth (PDB). Uji fermentasi dengan kapang Hypocreales sp. dan Diaporthe stewartii dan konsorsium serta kontrol. Hasil fermentasi dicek dengan TLC dan uji Fehling untuk mengetahui komposisi senyawa dan keberadaan mono-karbohidrat. Pakan udang yang difermentasi menunjukkan perbedaan tekstur dan warna. Ekstrak pakan fermentasi dengan Hypocreales sp. sebesar 2,69 g (3,36%), Diaporthe stewartii sebesar 4,9 g (6,13%), dan konsorsium sebesar 3,75 g (4,69%). Hasil TLC dan visualisasi vanillin sulfat tidak menunjukkan perbedaan jenis senyawa pada pakan kontrol dan pakan terfermentasi. Hasil uji Fehling menunjukkan bahwa kapang mampu mendegradasi selulosa yang dapat digunakan untuk meningkatkan kualitas pakan udang.

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The effects of different potato dextrose agar media on secondary metabolite production in Fusarium

Cited by 16 publications

References 30 publications

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

Fusarium pallidoroseum: A potential entomopathogenic agent for the biological management of Aphis gossypii

Degradasi Karbohidrat pada Pakan Udang oleh Isolat Kapang Endofit Mangrove

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