The effects of different levels of sodium diformate on growth performance, Immunological respond, digestive enzyme activity and intestinal histomorphology in juvenile Siberian Sturgeon Acipenser baerii

Abstract

Sodium diformate (NaDF) is organic acids that modulate growth performance, Immunological respond, digestive enzyme activity and intestinal histomorphology status. So, the present study aimed to investigate the effects of different levels of NaDF on growth performance, Immunological respond, digestive enzyme activity and intestinal histomorphology in juvenile Siberian Sturgeon Acipenser baerii. A total of 360 juvenile Acipenser baerii (34.55 ± 4.5 g) was randomly divided into 4 experimental treatments (Three replicates each). Juvenile Siberian Sturgeon fed control food or diet supplemented with different levels of NaDF (0. 5, 1 and 1.5 g/Kg) for 60 days. The results showed that concentration of 0.1% NaDF improved significantly (p < .05) fish growth performance following 30 days of application (p < .05). The results showed that dietary administration of NaDF significantly (p < .05) increased digestive enzymes. Dietary administration of NaDF in all treatments significantly increased the serum lysozyme and complement activity, and respiratory burst activity in A. baerii at days 60th of the experimental period. The highest bactericidal activity (P < 0.05) was observed in the fish which fed diet containing 0.15% NaDF (at days 30th ) and 0.1% NaDF (at days 60th ). The fishes fed diets supplemented with NaDF like other trail factor, presented increase in the thickness of the epithelium of the intestine, villus height, villus width, and number of goblet cells, were greatest in 0.15% NaDF, and followed by 0.1% NaDF after day 30 and 60 of culture. The present results revealed beneficial effects of 1 g/Kg dietary NaDF (0.1% NaDF) concentration on growth performance and physiological response of A. baerii for 60 days.