THE EFFECTS OF DIFFERENT IN VITRO AND EX VITRO TREATMENTS ON THE ROOTING PERFORMANCE OF PERSIAN WALNUT (Juglans regia L.) MICROSHOOTS

Saadat, Y A; Hennerty, M. J.

doi:10.17660/actahortic.2001.544.65

Cited by 9 publications

(4 citation statements)

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“…Whereas, with the same protocol using DKWC formulation, for nine clones of Mj209xRa values of mortality between 16.7 and 67.3% were registered Unlike the culture medium, IBA is used almost unanimously for walnut root induction, although variations can be found regarding the more suitable concentrations. Thus, for Persian walnut variety 'Serr' registered an important reduction of rooting using concentrations above 19 µM in 1 2 of DKW's macronutrients [57]. Whereas, Docet-Sanjuan et al [58] concluded that for rooting of hybrid 'A69' (J. nigra x J. regia) the optimum level of IBA might be localized between 32 and 100 µM also using DKW formulation but reducing macronutrients four times.…”

Section: Acclimatizationmentioning

confidence: 98%

Unraveling Factors Affecting Micropropagation of Four Persian Walnut Varieties

Yegizbayeva¹,

García-García

Yausheva³

et al. 2021

Agronomy

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Walnuts are considered recalcitrant to tissue culture, with a great genetic determinism on all stages of micropropagation; while other factors, also with great impact, become more complicated with the reproduction of newly realized varieties. In this research, a holistic approach aimed to determine the influence of genotype and the nutritive formulation throughout the whole cycle of micropropagation of four Persian walnut varieties (Juglans regia L.) was presented. During the in vitro establishment it was determined that besides genotype and culture medium, the effect of collection season and the likely interaction amongst factors had a great influence on the successful introduction of all four genotypes. However, all cultures were affected by a deep decay, being necessary the introduction of ethylenediamine di-2-hydroxyphenyl acetate ferric, as iron source, and Phloroglucinol in both Murashige and Skoog (1962) and the corrected Driver and Kuniyuki (1987) formulations. These modifications allowed the stabilization of cultures, maintaining thereafter a steady quality. Either proliferation, rooting and ex vitro survival of four clones were affected by the culture medium, obtaining the best results with the corrected Driver and Kuniyuki (1987) formulation. Finally, in vitro plants produced from all clones were acclimated with high survival rates (75.9–91.1% for the best culture medium), depending of clone and the culture medium used. Microsatellite analysis showed that micropropagated plants maintained the same genetic profiles of their corresponding donor trees. These results might contribute to deepening of the understanding of factors that determine the success of micropropagation of walnuts, and the extents of its influence; whereas, it sets the basis for the commercial micropropagation of all four clones.

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Section: Acclimatizationmentioning

confidence: 98%

Unraveling Factors Affecting Micropropagation of Four Persian Walnut Varieties

Yegizbayeva¹,

García-García

Yausheva³

et al. 2021

Agronomy

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“…Persian walnut is a difficult-to-root species. Rooting and acclimatization are the main factors limiting micropropagation (Chenevard et al, 1995;Ripetti et al, 1994;Saadat and Hennerty, 2001). In the last decade, walnut tree improvement programs have released promising new cultivars, but problems in rooting microshoots or conventional cuttings limit the development of new rootstocks and production of cultivars on their own roots (Navatel and Bourrain, 2001;Scaltsoyiannes et al, 1997).…”

mentioning

confidence: 99%

“…Genotypes varied in rooting ability. Saadat and Hennerty (2001) also examined several variations of this two-step procedure and obtained the best rooting by culturing microshoots on half-strength DKW medium supplemented with 20 µM IBA for 9 d in the dark, followed by transfer to Jiffy-7 pots.…”

mentioning

confidence: 99%

Rooting and Acclimatization of In Vitro-grown Shoots from Mature Trees of Three Persian Walnut Cultivars

Vahdati¹,

Leslie²,

Zamani³

et al. 2004

HortSci

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In vitro rooting of three commercial cultivars of Persian walnut (Juglans regia L.), `Sunland', `Chandler', and `Vina', was examined using a two-phase rooting procedure: root induction in the dark on Murashige and Skoog (MS) medium with 15 μm IBA followed by root development in the light on a mixture of one-quarter strength Driver Kuniyuki Walnut (DKW) basal medium and vermiculite (1:1.25, v/v). Rooting percentages were: `Sunland' (94%), `Chandler (55%), and `Vina' (27%). A positive relationship was observed between the vigor of cultivars and rooting ability, but shoot length did not affect rooting success. Rooting was optimum when shoots were cultured on root induction media for 6 to 8 days. Increasing the sucrose level in the root induction medium to 40 g·L-1 improved rooting, and shoots induced to root at 22 °C rooted more readily than those induced at 30 °C. Either increasing or decreasing the nitrogen level in the multiplication medium had a negative effect on rooting. Rooted walnut shoots often cease growth during acclimatization, resulting in shoot rosetting. Spray application of Promalin® (25 mL·L-1) caused buds to break and induced elongation of shoots. Chemical name used: indole-3-butyric acid (IBA).

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“…With respect to the rooting phase, Saadat and Hennerty (2001) obtained higher percentage of rooted microshoots with IBA as compared to naphthalene acetic acid (NAA). They also recommended carrying out the root induction under dark conditions (9 days) in presence of auxins in the culture media, which permit to obtain up to 83% rooted microshoots.…”

Section: Introductionmentioning

confidence: 99%